...Chao-Yin Cheng; Daniel P. Romero; Martin Zoltner; Meng-Chao Yao; Aaron P. Turkewitz ABSTRACT The contractile vacuole complex (CVC) is a dynamic and morphologically complex membrane organelle, comprising a large vesicle (bladder) linked with a tubular reticulum (spongiome). CVCs provide key...

... than 1.5 times higher than that of the cytosol. These findings indicate that excess cytosolic water crosses the contractile vacuole complex membrane osmotically. Substitution of choline or Ca 2+ for K + in the external solution or the external application of furosemide caused concomitant decreases...

...Agnes K. Fok; Marilynn S. Aihara; Masaki Ishida; Kathleen V. Nolta; Theodore L. Steck; Richard D. Allen ABSTRACT Our previous study has shown that the decorated tubules (collectively known as the decorated spongiome) of the contractile vacuole complex (CVC) in Paramecium are the site of fluid...

... antibodies. Using an affinity-purified antibody to calmodulin and a mono-specific antibody to the B subunit of the chromaffin granule vacuolar ATPase, markers known to label the membranes of the contractile vacuole complex in Dictyostelium (Zhu and Clarke, J. Cell Biol . 118, 347-358, 1992; Heuser et al., J...

...Richard D. Allen; Marilynn S. Ueno; Leonard W. Pollard; Agnes K. Fok ABSTRACT A monoclonal antibody (mAb) has been developed and selected by immunofluorescence for the radial canals of the contractile vacuole complex (CVC) of Paramecium multimicronucleatum . By applying indirect immunogold labeling...