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Keywords: Confocal microscopy
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Journal Articles
J Cell Sci (2021) 134 (14): jcs258332.
Published: 28 July 2021
... Fluorescence recovery after photobleaching Confocal microscopy Prostate cancer Dimerization is important for ARfl and ARv7 transcriptional activity ( van Royen et al., 2011 ; Xu et al., 2015 ). In the canonical ARfl pathway, binding of androgens induces a monomeric intramolecular N/C interaction...
Includes: Supplementary data
Journal Articles
J Cell Sci (2020) 133 (15): jcs248062.
Published: 6 August 2020
... Total internal reflection fluorescence microscopy Confocal microscopy Arabidopsis Clathrin-mediated endocytosis (CME) is a major mechanism by which plasma membrane (PM) and extracellular cargo, including cell surface receptors and extracellular materials, are internalized into cells...
Includes: Supplementary data
Journal Articles
J Cell Sci (2018) 131 (21): jcs215517.
Published: 8 November 2018
... Microcluster Confocal microscopy Highlighted Article: During T cell activation, a cytoskeletal pool of ADAP enters nascent contacts in advance of other adaptors and enhances contact stability and TCR signaling by participating in multivalent signaling complexes. © 2018. Published by The Company...
Includes: Supplementary data
Journal Articles
J Cell Sci (2016) 129 (5): 893–897.
Published: 1 March 2016
... µM of each) in buffer containing 1 mM CaCl 2 was added to subconfluent BHK21 cells and incubated for 1 h, after which cells were washed and imaged by fluorescence confocal microscopy. Uptake of cargo into cell interiors was assayed using an inverted Zeiss (Jena, Germany) LSM700 confocal microscope...
Journal Articles
J Cell Sci (2015) 128 (21): 3888–3897.
Published: 1 November 2015
.... Gap junction Confocal microscopy Connexin Membrane trafficking Pulse-chase Tracer coupling Throughout the central nervous system, neurons are coupled to each other by electrical synapses, which consist of gap junctions that provide a direct intercellular pathway for current flow...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (15): 2795–2804.
Published: 1 August 2015
... Highlighted Article: Confocal live-cell imaging combined with correlative cryogenic fluorescence microscopy and soft X-ray tomography provide new insights into the complex process of mitochondrial fission in mammalian cells. Confocal microscopy Endoplasmic reticulum Microscopy imaging Mitochondria...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (3): 441–446.
Published: 1 February 2015
... ), while type 1 nodes move away from the equator as their proteins disperse into the cytoplasm for the duration of mitosis, a time called the eclipse period ( Akamatsu et al., 2014 ). Cytokinesis Fission yeast Interphase nodes Septation initiation network Confocal microscopy © 2015...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (23): 5105–5114.
Published: 1 December 2014
... considered significant when P <0.05). Data are expressed as mean±s.e.m. obtained from experiments performed in a number ( n ) of individual cells from at least three different cultures. Exocytosis Chromaffin cell Mitochondria Endoplasmic reticulum Confocal microscopy Adrenomedullar...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (6): 900–909.
Published: 15 March 2011
... the constructs STAT3-ΔNLS–CY, STAT3-ΔNES–CY, STAT3-VL–CY and STAT3-ΔNT–CY. * Author for correspondence ( mueller-newen@rwth-aachen.de ) 15 10 2010 © 2011. 2011 STAT3 Dimerization Nucleocytoplasmic shuttling Confocal microscopy Dual-focus FCS The JAK/STAT (Janus tyrosine...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (14): 2512–2520.
Published: 15 July 2010
... receptor MDCK Basolateral targeting Confocal microscopy Charged amino acids C-terminal tail Extracellular nucleotides are released from essentially all cells and interact with P2 receptors to produce a broad range of physiological responses. P2 receptors are divided into two major classes: P2X...
Includes: Supplementary data
Journal Articles
J Cell Sci (2009) 122 (10): 1607–1615.
Published: 15 May 2009
... © The Company of Biologists Limited 2009 2009 Lens Green fluorescent protein Fluorescence correlation spectroscopy Lim2 Syncytium Confocal microscopy The generation of syncytia by cell fusion is an important stage in the development of many eukaryotic organisms and generally...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2006) 119 (7): 1255–1265.
Published: 1 April 2006
... for correspondence (e-mail: bsj@isv.cnrs-gif.fr ) 14 12 2005 © The Company of Biologists Limited 2006 2006 Plant cell polarity PIN proteins Endomembrane Cytoskeleton Immunocytochemistry Confocal microscopy A widely accepted model proposes that gradients of the hormone auxin...
Journal Articles
J Cell Sci (2005) 118 (20): 4797–4812.
Published: 15 October 2005
... of the actin-coated intermediates formed close to the APM, some could be detected deep within the cytoplasm. * Author for correspondence (e-mail: shalvar@usc.edu ) 6 7 2005 © The Company of Biologists Limited 2005 2005 Secretion Fluorescence recovery after photobleaching Confocal...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2005) 118 (13): 2871–2880.
Published: 1 July 2005
... images could be used as an alternative to fluorescence in the study of cytoskeleton dynamics in neuroendocrine cells. * Author for correspondence (e-mail: Luisguti@umh.es ) 5 4 2005 © The Company of Biologists Limited 2005 2005 Exocytosis Confocal microscopy Adrenomedullary...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2005) 118 (8): 1587–1594.
Published: 15 April 2005
...), and cells were embedded in 90% buffered glycerol in PBS for confocal microscopy (μRadiance confocal scanning system; Bio-Rad, UK). For AFM, HUVECs were grown onto coverslides and heat-stressed as described. After a 6-hour recovery period at 37°C, they were fixed with 2% glutaraldehyde. Incubation...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2004) 117 (19): 4517–4526.
Published: 1 September 2004
... studied by confocal microscopy using a monoclonal antibody directed against p75. p75* is localised in the apical membrane of MDCK cells, whereas the chimeric construct p75*-Q1(Y51) is mainly located in the basolateral membrane. Mutation of Y51 into an alanine eliminates the ability of the KCNQ1 sequence...
Journal Articles
J Cell Sci (2003) 116 (12): 2495–2503.
Published: 15 June 2003
... Signal transduction Fluorescent protein fusions Confocal microscopy There is increasing evidence that cross-talk between signalling pathways is essential to modulate the cellular response to external stimuli. Understanding how these pathways mesh together has major implications for cell biology...
Journal Articles
J Cell Sci (2003) 116 (12): 2483–2494.
Published: 15 June 2003
... binding and enzymatic assays, confocal microscopy and amino acid sequence analysis. [ 3 H]-ryanodine binding in total membranes was enhanced by AMP-PCP, caffeine and xanthine, whereas Mg 2+ , Ruthenium Red and dantrolene were inhibitors.[ 3 H]-ryanodine binding showed a bell-shaped curve with increasing...
Journal Articles
J Cell Sci (2003) 116 (8): 1437–1447.
Published: 15 April 2003
... of Biologists Limited 2003 2003 Apoptosis Mitochondria Photosensitisation Confocal microscopy Chloromethyl-X-Rosamine Mitochondria have multiple roles in cells, including participation in bioenergetics, signalling and apoptosis. Two key events associated with mitochondrial involvement...
Journal Articles
J Cell Sci (2002) 115 (23): 4661–4669.
Published: 1 December 2002
...: ross.s@uq.edu.au ) 3 9 2002 © The Company of Biologists Limited 2002 2002 RNA-protein interactions Zipcode RNA trafficking Protein sequencing Confocal microscopy Localization of cellular proteins is accomplished by diverse pathways,including the trafficking and localised...