... with expansion of liver progenitor cells. In response to chronic CCl 4 -induced liver injury, the pattern of deposited collagen was significantly altered in these mice. The liver injury marker alpha-fetoprotein (AFP) was increased in the secretome of VLK-deficient cultured progenitor cells and in liver tissues...

... will concentrate on two major classes of ECM proteins as case studies, namely the proteoglycans and fibrillar collagens. The Golgi is also relevant to matrix degradation, but this is not the focus here. Fig. 1. Electron microscopy of the Golgi in mouse tendon fibroblast cells. (A,B) Images of two separate...

... of peptide bonds. It has been shown that, upon eIF5A depletion, yeast ribosomes stall in polyproline motifs, but also in tripeptide sequences that combine proline with glycine and charged amino acids. Mammalian collagens are enriched in putative eIF5A-dependent Pro-Gly-containing tripeptides. Here, we show...

.... Summary: TANGO1 is required to maintain the fundamental organization of the early secretory pathway in mammalian cells to support secretion of a diverse range of newly synthesized cargo proteins. Secretory pathway COPII Collagen Golgi TANGO1 ERGIC University of Bristol http...

..., which is independent of the increasing epidermal length or the developmental cycles. This long-range synchronized reorganization of subcellular structures is achieved by physical links established by extracellular collagens together with extension forces generated from epidermal cell growth. Our studies...

... that cells can be mechanically conditioned. Owing to increased focal adhesion assembly, Inv L /E:N H cells migrated faster, which could be reduced when increasing integrin affinity with high divalent cation concentrations. Mirroring these data in human patients, we observed that collagen organization...

... of diverse animal tissues and organs. A major ECM component are members of the collagen superfamily –comprising 28 types in vertebrates – that exist in diverse supramolecular assemblies ranging from networks to fibrils. Each assembly is characterized by a hallmark feature, a protein structure called a triple...

...Daniela Semeniak; Rebecca Kulawig; David Stegner; Imke Meyer; Silke Schwiebert; Hendrik Bösing; Beate Eckes; Bernhard Nieswandt; Harald Schulze ABSTRACT Collagen receptors GPVI (also known as GP6) and integrin α2β1 are highly expressed on blood platelets and megakaryocytes, their immediate...

...Sindhu Row; Yayu Liu; Stella Alimperti; Sandeep K. Agarwal; Stelios T. Andreadis ABSTRACT We discovered that Cadherin-11 (CDH11) regulates collagen and elastin synthesis, both affecting the mechanical properties and contractile function of animal tissues. Using a Cdh11 -null mouse model, we...

... of matrix receptors on the formation, and dynamic and degradative activities of invadosomes. In particular, we highlight recent findings regarding the role of type I collagen fibrils in inducing the formation of a new linear organisation of invadosomes. We will also discuss invadosome plasticity more...

... affinity to collagen I and to collagen XII that decorates the surface of collagen I fibrils. We demonstrate here that lack of COMP–collagen interaction in the extracellular space leads to changes in collagen fibril morphology and density, resulting in altered skin biomechanical properties. Surprisingly...

...Cédric Zeltz; Donald Gullberg ABSTRACT The α1β1, α2β1, α10β1 and α11β1 integrins constitute a subset of the integrin family with affinity for GFOGER-like sequences in collagens. Integrins α1β1 and α2β1 were originally identified on a subset of activated T-cells, and have since been found...

... to initiate sprouting responses in activated endothelial cells. Collagen Endothelial cell Angiogenesis Three-dimensional Cell migration Matrix degradation MT1-MMP FAK S1P LIM Angiogenesis is defined as new blood vessel growth from pre-existing structures ( Folkman and D'Amore, 1996...

...Matilde Cescon; Francesca Gattazzo; Peiwen Chen; Paolo Bonaldo ABSTRACT Collagen VI represents a remarkable extracellular matrix molecule, and in the past few years, studies of this molecule have revealed its involvement in a wide range of tissues and pathological conditions. In addition to its...

... collagen matrices; however, its regulation during tubulogenesis is not understood. Here, we report that degradation of collagen in polarized epithelial cells is post-translationally regulated by changing the localization of MT1-MMP from the apical to the basal surface. MT1-MMP predominantly localizes...

... but lacked multiple collagen-processing enzymes, including lysyl oxidase ( Lox ), a BMP2-responsive gene regulated by Smad4 and Runx2. Accordingly, the collagen matrix in Smad4 mutants was disorganized, but also hypomineralized. Primary osteoblasts from these mutants did not mineralize in vitro...

... for genes with large 3′ terminal exons, and the applicability of the current models to large multi-exon genes is not clear. In this Commentary, we present an overview of the current understanding of NMD and discuss how analysis of nonsense mutations in the collagen gene family has provided new mechanistic...

... mice and mice carrying a chondrocyte-specific Col2a1–Cre transgene. Hsp47 conditional null mutant mice died just before or shortly after birth, and exhibited severe generalized chondrodysplasia and bone deformities with lower levels of type II and type XI collagen. Second-harmonic generation (SHG...

... CRMs associated with the structural muscle gene fibrillar collagen 1 ( CiFCol1 ). We use these representative examples to reconstruct how compact CRMs orchestrate the muscle developmental program from pre-localized ooplasmic determinants to differentiated larval muscle in ascidian embryos...

... . The joint action of both contraction modes can result in macroscopic tissue contractures of ~1 cm per month. * Author for correspondence ( boris.hinz@utoronto.ca ) 5 3 2010 © 2010. 2010 Myofibroblast Fibrosis Calcium oscillations Rho kinase Stress fibre Collagen...