...). Cytochalasin D and colchicine (Calbiochem-Novabiochem, Nottingham, UK) were used at final concentrations of 2.0 μM and 2.5 μM, respectively. Control experiments were performed with the vehicle alone; DMSO for cytochalasin D and ethanol for colchicine. In some experiments, cells were plated on dishes precoated...

...Carrie R. Cowan; W. Zacheus Cande Telomere clustering, the defining feature of the bouquet, is an almost universal feature of meiotic prophase, yet its mechanism remains unknown. The microtubule-depolymerizing agent colchicine was found to inhibit bouquet formation. Telomeres in colchicine-treated...

.../centrioles for the spindle microtubules. When replicating tachyzoites were treated with 0.5 μM oryzalin or 1.0 mM colchicine they retained the capacity to form a spindle and undergo nuclear division. Although these parasites could complete budding,they lost the bulk of their subpellicular microtubules...