... Basto Competing interests The authors declare no competing or financial interests. 22 4 2024 3 2 2025 © 2025. Published by The Company of Biologists 2025 https://www.biologists.com/user-licence-1-1/ Summary: Mis4, which acts as a cohesin loader during...

... lipid metabolism genes. We show that in cbf11Δ cells mitotic defects have already appeared prior to anaphase, before the nuclear expansion begins. Moreover, we identify altered cohesin dynamics and centromeric chromatin structure as additional factors affecting mitotic fidelity in cells with disrupted...

... cohesion establishment and mitotic cohesion dissolution have been extensively explored, the regulation of cohesin loading is still poorly understood. Here, we report that the methyltransferase NSD3 is essential for mitotic sister chromatid cohesion before mitosis entry. NSD3 interacts with the cohesin...

.... In the fission yeast, Schizosaccharomyces pombe , Rec8-containing meiotic cohesin is suggested to establish kinetochore associations by mediating cohesion of the centromere cores. However, cohesin-mediated kinetochore associations on intact chromosomes have never been demonstrated directly. In the present study...

...Avi Matityahu; Itay Onn ABSTRACT The three-dimensional structure of chromatin is determined by the action of protein complexes of the structural maintenance of chromosome (SMC) family. Eukaryotic cells contain three SMC complexes, cohesin, condensin, and a complex of Smc5 and Smc6. Initially...

...Su Ming Sun; Amandine Batté; Mireille Elmer; Sophie C. van der Horst; Tibor van Welsem; Gordon Bean; Trey Ideker; Fred van Leeuwen; Haico van Attikum ABSTRACT Eukaryotic chromosomes are replicated in interphase and the two newly duplicated sister chromatids are held together by the cohesin complex...

...Robert V. Skibbens ABSTRACT Condensins and cohesins are highly conserved complexes that tether together DNA loci within a single DNA molecule to produce DNA loops. Condensin and cohesin structures, however, are different, and the DNA loops produced by each underlie distinct cell processes...

...Swastika Sanyal; Lucia Molnarova; Judita Richterova; Barbora Huraiova; Zsigmond Benko; Silvia Polakova; Ingrid Cipakova; Andrea Sevcovicova; Katarina Gaplovska-Kysela; Karl Mechtler; Lubos Cipak; Juraj Gregan ABSTRACT The canonical role of cohesin is to mediate sister chromatid cohesion...

...Peter G. Wolf; Alexander Cuba Ramos; Julia Kenzel; Brigitte Neumann; Olaf Stemmann ABSTRACT The DNA-embracing, ring-shaped multiprotein complex cohesin mediates sister chromatid cohesion and is stepwise displaced in mitosis by Wapl and separase (also known as ESPL1) to facilitate anaphase. Proper...

...Christopher Bot; Annika Pfeiffer; Fosco Giordano; Dharani E. Manjeera; Nico P. Dantuma; Lena Ström ABSTRACT NIPBL is required to load the cohesin complex on to DNA. While the canonical role of cohesin is to couple replicated sister chromatids together until the onset of mitosis, it also promotes...

... that paxillin interacts with cohesin and the mediator complex, which have been shown to mediate long-range chromosomal looping. We propose that these interactions occur at the IGF2 and H19 gene cluster and are involved in the formation of loops between the IGF2 and H19 promoters and the enhancer, and thus...

... explanation for the contribution of lamins and cohesins to meiotic recombination. I thank Dale Dorsett, Maurizio Gatti, R. Scott Hawley, Kim McKim, Yikang Rong, Gertrud Schüpbach, the Bloomington Stock Center (Bloomington, IN, USA), and the Developmental Studies Hybridoma Bank for fly stocks and reagents...

... for chromatin assembly of the male pronucleus, where it acts as a histone H3.3 chaperone in complex with Hira. We report here that Yem associates with the synaptonemal complex and the cohesin complex. A genetic interaction between yem 1 (V478E) and the Spo11 homolog mei-W68 , modified a yem 1 dominant effect...

...Maria T. Ocampo-Hafalla; Frank Uhlmann Cohesin is best known as a crucial component of chromosomal stability. Composed of several essential subunits in budding yeast, cohesin forms a ring-like complex that is thought to embrace sister chromatids, thereby physically linking them until their timely...

...Lily Hui-Ching Wang; Bernd Mayer; Olaf Stemmann; Erich A. Nigg Sister chromatid cohesion is mediated by DNA catenation and proteinaceous cohesin complexes. The recent visualization of PICH (Plk1-interacting checkpoint helicase)-coated DNA threads in anaphase cells raises new questions...

... subunit of cohesin, is a key target of separase in yeast. Is the equivalent protein also a target in mammals? We show here that separase cleaves mouse Rec8 at three positions in vitro but only when the latter is hyper-phosphorylated. Expression of a Rec8 variant (Rec8-N) that cannot be cleaved in vitro...

... clusters and at imprinted loci, and X-chromosome inactivation. Here, we review recent data acquired with state-of-the-art technologies that illuminate possible mechanisms behind the diversity of CTCF functions. CTCF interacts with numerous protein partners, including cohesin, nucleophosmin, PARP1, Yy1...

...Laura A. Díaz-Martínez; Juan F. Giménez-Abián; Duncan J. Clarke Sister-chromatid cohesion is essential for accurate chromosome segregation. A key discovery towards our understanding of sister-chromatid cohesion was made 10 years ago with the identification of cohesins. Since then, cohesins have...

... cohesion, although how ORD protein promotes cohesion has remained elusive. We show that SMC subunits of the cohesin complex colocalize with ORD at centromeres of ovarian germ-line cells. In addition, cohesin SMCs and ORD are visible along the length of meiotic chromosomes during pachytene and remain...

.... * Author for correspondence (e-mail: [email protected] ) 15 5 2007 © The Company of Biologists Limited 2007 2007 CDK11 p58 Cell cycle Mitosis Cyclin-dependent kinase Sister chromatid cohesion Cohesin Mouse Cyclin-dependent kinases (CDKs) are involved in a variety...