... into RTCs, which were subsequently functionalized by Rabin8 Rab8 GEF activity. Non-phosphorylatable GFP–Rabin8-S272A caused significant GES enlargement and deformation, possibly leading to unconventional membrane advancement toward the cilium, bypassing RTCs. Rabin8 phosphorylation loss due to an NDR2 gene...

... reveal that the GID complex is an integral part of the cilium required for primary cilia-dependent signal transduction and the maintenance of ciliary protein homeostasis. We show that GID complex subunits localize to cilia in both Xenopus laevis and NIH3T3 cells. Furthermore, we report SHH signaling...

... doublet decoration, particularly the inner dynein arms, are likely important ( Bui et al., 2009 , 2012 ). In addition to the asymmetric 9+2 axoneme, cilium-associated structures tend to be asymmetric. This includes the anchoring of the basal body to the cell by rootlet structures – such as the basal body...

...Abhishek Chadha; Stefanie Volland; Natella V. Baliaouri; Elaine M. Tran; David S. Williams ABSTRACT The photoreceptor outer segment is the most elaborate primary cilium, containing large amounts of rhodopsin (RHO) in disk membranes that grow from a connecting cilium. The movement of RHO along...

... provide a ‘cradle’ for the longin SNARE VAMP7 mediating its incorporation into prebudding complex at the Golgi/TGN during sensory receptor ciliary trafficking. VAMP7 Cilium SNARE Rab GTPase Sensory receptor Rhodopsin National Eye Institute 10.13039/100000053 EY-12421...

... GEF to activate a cognate Arf directing transport to the cilia. Cilium Arf GTPases Sensory receptors Rhodopsin National Eye Institute 10.13039/100000053 EY-12421 The Arf family of small G-proteins constitutes a crucial component of the intracellular membrane trafficking...

...Xuefeng Su; Maoqing Wu; Gang Yao; Wassim El-Jouni; Chong Luo; Azadeh Tabari; Jing Zhou ABSTRACT Failure to localize membrane proteins to the primary cilium causes a group of diseases collectively named ciliopathies. Polycystin-1 (PC1, also known as PKD1) is a large ciliary membrane protein...

... ) Competing interests The authors declare no competing or financial interests. 5 09 2014 3 02 2015 © 2015. Published by The Company of Biologists Ltd 2015 Cilium Rab11a Rhodopsin Nearly all eukaryotic cells contain exquisitely organized membrane projections called primary...

... member of a widely conserved family of MAP6-related proteins specific to centrioles and cilium microtubules. Our data demonstrate that hSAXO1 binds specifically to centriole and cilium microtubules. We identify, in vivo and in vitro , hSAXO1 Mn modules as responsible for microtubule binding...

...Anne Aubusson-Fleury; Michel Lemullois; Nicole Garreau de Loubresse; Chloé Laligné; Jean Cohen; Olivier Rosnet; Maria Jerka-Dziadosz; Janine Beisson; France Koll Summary Within the FOP family of centrosomal proteins, the conserved FOR20 protein has been implicated in the control of primary cilium...

..., but their specific fusion sites have not been characterized. Here, we report that the Sec6/8 complex, or exocyst, is a candidate effector for Rab8. We also show that the Qa-SNARE syntaxin 3 is present in the rod inner segment (RIS) plasma membrane at the base of the cilium and displays a microtubule-dependent...

... between cilia and cell cycle. The primary cilium expressed by most mammalian cells is nucleated from the elder centriole of the centrosome. The relationship between centrosome and cilia suggests that these structures share functions and components. We now show that IFT88/polaris, a component...

... cells. These data support a specific role for dynein 2 in the generation and maintenance of cilia. © The Company of Biologists Limited 2002 2002 Dynein Cilium Microtubule Retina Light intermediate chain Brain Cytoplasmic dynein is a minus end-directed microtubule motor protein...

... 2000 by Company of Biologists 2000 Dynein Cilium Flagellum Phosphatase PP1 PP2A, Chlamydomonas Cell motility The major challenge in understanding ciliary and flagellar motility is to determine the mechanisms that locally control the multiple dynein motors ( Satir, 1985 ; Brokaw...

... ). Alpha tubulin Assembly Axoneme Chlamydomonas reinhardtii Cilium Detyrosination Flagellum Microtubule Tubulin carboxypeptidase Tyrosinated tubulin © 1998 by Company of Biologists 1998 24 10 1997 15 01 1998 ...

... Molecular motor ATPase Microtubule Cilium Flagellar radial spoke Dynein regulatory complex © 1996 by Company of Biologists 1996 06 02 1996 28 03 1996 * Author for correspondence (e-mail: [email protected] ) ...

... et al., 1993 ; Eshel et al., 1993 ). axonal transport motor protein RT-PCR dynein microtubule-ATPase flagellum cilium microtubule phylogeny molecular evolution © 1995 by Company of Biologists 1995 19 09 1994 17 02 1995 * Y.T. and Z.Z. made equal...

...+ in single cells. Ovine tracheal epithelial cells, obtained by dissociation with protease, were grown in primary culture for 1 to 28 days in a mucus-free system. CBF of a single cilium was measured by digital video phase-contrast microscopy and on-line Fourier-transform analysis. Changes in [Ca 2+ ] i from...

... of Biologists 1994 cell motility cilium microtubule cytoplasmic transport molecular motor Although much is known about dynein ATPases and their roles in cilia and flagella ( Witman, 1992 ), the presence of other microtubule-associated molecular motors has not been determined. In this work...

... School, Piscataway, NJ 08854, USA 23 07 1993 12 10 1993 © 1994 by Company of Biologists 1994 dynein dynein isoform catalytic P-loop Paramecium cilium The dyneins are a family of very high molecular mass motor enzymes that produce directed movement along...