... that centromere clustering may have been the primordial mechanism for chromosome pairing in early eukaryotes. MS analyses were performed by the Mass Spectrometry Facility of the Max F. Perutz Laboratories using the VBCF instrument pool. We thank Markus Hartl and his team for their support in proteomics analysis...

... ’s stretched bouquet and, thereby, for homologue pairing. This finding adds to previous reports of the importance of centromeres in chromosome pairing in budding yeast and in Drosophila . Tetrahymena ’s bouquet is an ataxia telangiectasia- and RAD3-related (ATR)-dependent meiotic DNA damage response...

... leptonema showed a prolonged bouquet-stage configuration, asynapsis and heterosynapsis, suggesting function(s) of STRA8 in chromosome pairing. Most importantly, a large number of mutant leptotene spermatocytes underwent premature chromosome condensation, within 24 hours following the meiotic S phase...

... reduction in chromosome pairing, but show a complete failure to form chiasmata and exhibit chromosome missegregation. The lack of SPO11 prevents the elongation of meiotic nuclei, but it is restored by the artificial induction of DSBs. In the hop2A Δ mutant, the transient appearance of γ-H2A.X and Rad51p...

... of exchanges. * Author for correspondence (e-mail: l.mullenders@lumc.nl ) 3 2 2005 © The Company of Biologists Limited 2005 2005 Chromosome pairing Chromosome positioning Cross-link repair Heterochromatin MMC XPF Mitomycin C (MMC) is a natural antitumor antibiotic...

...Josef Loidl; Harry Scherthan During meiotic prophase in the ciliate Tetrahymena thermophila micronuclei dramatically elongate and form thread-like crescents. The arrangement of the chromosomes within the crescent as well as the timing of chromosome pairing and recombination with respect...

... of telomere clustering and initiation of chromosome synapsis has led to the hypothesis that the bouquet facilitates homologous chromosome pairing and synapsis. However, recent mutant analysis suggests that the bouquet is not absolutely required for either homologous pairing or synapsis but that it makes both...

... of the meiosis-induced rec10 gene of Schizosaccharomyces pombe. Curr. Genet. 27 , 440 -446. Loidl, J. ( 1990 ). The initiation of meiotic chromosome pairing: the cytological view. Genome 33 , 759 -778. Loidl, J. ( 1994 ). Cytological aspects of meiotic recombination. Experientia 50...