...Koichiro Maki; Michele M. Nava; Clémentine Villeneuve; Minki Chang; Katsuko S. Furukawa; Takashi Ushida; Sara A. Wickström ABSTRACT Articular cartilage protects and lubricates joints for smooth motion and transmission of loads. Owing to its high water content, chondrocytes within the cartilage...

... −/− mice were similar to those in Panx3 −/− mice. The gross appearance of newborn Cx43 −/− skeletons showed no obvious abnormalities, except for less mineralization of the skull. In Panx3 −/− mice, proliferation of chondrocytes and osteoblasts increased and differentiation of these cells was inhibited...

... and cartilage. Our team previously identified 14-3-3ε protein as a subchondral bone soluble mediator altering cartilage homeostasis. The aim of this study was to investigate the involvement of CD13 (also known as aminopeptidase N, APN) in the chondrocyte response to 14-3-3ε. After identifying CD13...

... development as deletion of Tak1 in chondro-osteo progenitor cells leads to severe chondrodysplasia with defects in both chondrocyte proliferation and maturation. We have investigated the role of TAK1 in committed chondrocytes during early postnatal development. Using the Col2a1-CreER T2 ; Tak1 f/f mouse model...

...Bing Shu; Ming Zhang; Rong Xie; Meina Wang; Hongting Jin; Wei Hou; Dezhi Tang; Stephen E. Harris; Yuji Mishina; Regis J. O'Keefe; Matthew J. Hilton; Yongjun Wang; Di Chen The BMP signaling pathway has a crucial role in chondrocyte proliferation and maturation during endochondral bone development...

...Guoyan Wang; Qian Yan; Anita Woods; Laura A. Aubrey; Qingping Feng; Frank Beier Coordinated proliferation and differentiation of growth plate chondrocytes controls endochondral bone growth and final height in humans, and disruption of this process results in diseases of the growing and adult...

... cells. These results identify Wif-1 as a novel extracellular Wnt modulator in cartilage biology. * Author for correspondence ( [email protected] ) 18 7 2009 © The Company of Biologists Limited 2009 2009 Wif-1 Wnt signalling β-catenin Chondrocyte Chondrogenesis...

...Ming Zhang; Rong Xie; Wei Hou; Baoli Wang; Run Shen; Xiumei Wang; Qing Wang; Tianhui Zhu; Jennifer H. Jonason; Di Chen In chondrocytes, PTHrP maintains them in a proliferative state and prevents premature hypertrophy. The mechanism by which PTHrP does this is not fully understood. Both Runx2...

... death. To address the role of β-catenin in postnatal skeletal growth and development, Col2a1-ICAT transgenic mice were generated. Mice were viable and had normal size at birth, but became progressively runted. Transgene expression was limited to the chondrocytes in the growth plate and articular...

... growth. It is believed that FGFR3 inhibits chondrocyte proliferation via activation of signal transducers and activators of transcription (STAT) proteins, although the exact mechanism of both STAT activation and STAT-mediated inhibition of chondrocyte growth is unclear. We show that FGFR3 interacts...

... signaling in chondrocytes. CNP antagonized FGF2-induced growth arrest of rat chondrosarcoma (RCS) chondrocytes by inhibition of the Erk mitogen activated protein kinase pathway. This effect of CNP was protein kinase G-dependent and was mimicked by the cGMP analog pCPT-cGMP. FGF2-mediated activation of both...

...Hirayuki Enomoto; Tatsuya Furuichi; Akira Zanma; Kei Yamana; Carolina Yoshida; Satoru Sumitani; Hiroyasu Yamamoto; Motomi Enomoto-Iwamoto; Masahiro Iwamoto; Toshihisa Komori Runx2 (runt-related transcription factor 2) is an important transcription factor for chondrocyte differentiation as well...

... expression,using cultures of chondrocytes and MTf-overexpressing ATDC5 and C3H10T1/2 cells. In cultures expressing MTf at high levels, concanavalin A induced cell-shape changes from fibroblastic to spherical cells, whereas no cell-shape changes were observed with wild-type ATDC5 or C3H10T1/2 cells expressing...

... ). Reporter constructs with low background activity utilizing the cat gene. Gene 110 , 129 -130. Bridgewater, L. C., Lefebvre, V. and De Crombrugghe, B. ( 1998 ). Chondrocyte-specific enhancer elements in the Col1 1a2 gene resemble the Col2a1 tissue-specific enhancer. J. Biol. Chem. 273 , 14998...

...David P. Thomas; Andrew Sunters; Aleksandra Gentry; Agamemnon E. Grigoriadis ABSTRACT We have investigated the role of c-Fos in chondrocyte differentiation in vitro using both constitutive and inducible overexpression approaches in ATDC5 chondrogenic cells, which undergo a well-defined sequence...

... is permissive for the proliferation and differentiation of chondrocytes. Mouse embryonic day 10 (E10) limb buds were microdissected and dissociated into cells which were then cultured within a collagen gel matrix and maintained for up to 10 days. Static compressive forces were exerted onto these cultures...

...Thomas M. Quinn; Alan J. Grodzinsky; Michael D. Buschmann; Young-Jo Kim; Ernst B. Hunziker ABSTRACT We have used new techniques of cell-length scale quantitative autoradiography to assess matrix synthesis, deposition, and deformation around individual chondrocytes in mechanically compressed...

...Patrizio Castagnola; Massimo Gennari; Roy Morello; Laura Tonachini; Oriano Marin; Alessia Gaggero; Ranieri Cancedda ABSTRACT A subtracted cDNA library was generated to identify cDNAs specific for chondrocyte mRNAs preferentially expressed at the hypertrophic stage with respect to early...

...Prasit Pavasant; Tony Shizari; Charles B. Underhill ABSTRACT Histochemical staining of the epiphysial growth plate revealed that free hyaluronan (i.e. available to the staining probe) was restricted to the zone of hypertrophy, where it was located in the pericellular space between the chondrocytes...

...Michael D. Buschmann; Ernst B. Hunziker; Young-Jo Kim; Alan J. Grodzinsky ABSTRACT Previous studies have shown that static equilibrium compression of cartilage tissue in vivo and in vitro decreases chondrocyte synthesis of aggrecan molecules. In order to identify mechanisms of cellular response...