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Keywords: Chlamydomonas
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Journal Articles
In collection:
Cilia and Flagella
J Cell Sci (2022) 135 (10): jcs259814.
Published: 23 May 2022
... receptors are well studied, fundamental questions remain about the mechanisms and molecules that transduce ciliary signals into responses in the cytoplasm. During fertilization in the bi-ciliated alga Chlamydomonas reinhardtii , ciliary adhesion between plus and minus gametes triggers an immediate ∼10-fold...
Includes: Supplementary data
Journal Articles
In collection:
Cilia and Flagella
J Cell Sci (2021) 134 (3): jcs253492.
Published: 11 February 2021
...Cecile Fort; Peter Collingridge; Colin Brownlee; Glen Wheeler ABSTRACT The movement of ciliary membrane proteins is directed by transient interactions with intraflagellar transport (IFT) trains. The green alga Chlamydomonas has adapted this process for gliding motility, using retrograde IFT motors...
Includes: Supplementary data
Journal Articles
In collection:
Cilia and Flagella
J Cell Sci (2019) 132 (16): jcs233429.
Published: 19 August 2019
...Robert A. Bloodgood; Joseph Tetreault; Roger D. Sloboda ABSTRACT In addition to bend propagation for swimming, Chlamydomonas cells use their flagella to glide along a surface. When polystyrene microspheres are added to cells, they attach to and move along the flagellar surface, thus serving...
Includes: Supplementary data
Journal Articles
J Cell Sci (2019) 132 (6): jcs229047.
Published: 15 March 2019
..., methylation, phosphorylation and ubiquitylation, participate in ciliary disassembly. However, the detailed mechanism and the role of ubiquitylation in ciliary disassembly are unclear. This study identified 20 proteins that were ubiquitylated in shortening flagella of Chlamydomonas . α-Tubulin was the most...
Includes: Supplementary data
Journal Articles
In collection:
Cilia and Flagella
J Cell Sci (2017) 130 (9): 1662–1674.
Published: 1 May 2017
... identify the region in IFT46 that is responsible for its basal body and ciliary localization. This sequence promotes interaction with IFT52, which recruits IFT46 to the basal body. Chlamydomonas Intraflagellar transport Basal body Targeting sequence Eukaryotic cells evolved different...
Includes: Supplementary data
Journal Articles
J Cell Sci (2016) 129 (15): 3008–3014.
Published: 1 August 2016
.... Here, we show that the six-transmembrane K + channel KCN11 in Chlamydomonas is exclusively localized to contractile vacuole. Ectopic expression of KCN11 in HEK293T cells results in voltage-gated K + channel activity. Disruption of the gene or mutation of key residues for K + permeability of the channel...
Includes: Supplementary data
Journal Articles
J Cell Sci (2016) 129 (10): 2106–2119.
Published: 15 May 2016
...-homology domain within the IFT81 N-terminus and the highly basic N-terminus of IFT74 form a module for IFT of tubulin. By using Chlamydomonas mutants for IFT81 and IFT74, we tested this hypothesis in vivo . Modification of the predicted tubulin-binding residues in IFT81 did not significantly affect basic...
Includes: Supplementary data
Journal Articles
In collection:
Cilia and Flagella
J Cell Sci (2016) 129 (5): 943–956.
Published: 1 March 2016
... evolutionary roots for bioactive peptide signaling. We demonstrate that the Chlamydomonas reinhardtii PAM gene encodes an active peptide-amidating enzyme (CrPAM) that shares key structural and functional features with the mammalian enzyme, indicating that components of the peptide biosynthetic pathway predate...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (21): 4714–4727.
Published: 1 November 2014
... about the function of NPHP4, we have studied it in Chlamydomonas reinhardtii . NPHP4 is stably incorporated into the distal part of the flagellar transition zone, close to the membrane and distal to CEP290, another transition zone protein. Therefore, these two proteins, which are incorporated...
Includes: Supplementary data
Journal Articles
J Cell Sci (2014) 127 (2): 281–287.
Published: 15 January 2014
...Dan Meng; Muqing Cao; Toshiyuki Oda; Junmin Pan ABSTRACT Eukaryotic flagella and cilia can exhibit planar and non-planar beating, and the mechanism controlling these beating patterns is not well understood. Chlamydomonas reinhardtii flagella beat in approximately the same plane with either...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (6): 1531–1540.
Published: 15 March 2013
..., the identity and regulation of this ‘pool’ is not understood. Previously, we have shown that Chlamydomonas kinesin-13 (CrKin13) is phosphorylated during flagellar regeneration, and required for proper flagellar assembly. In the present study, we show that CrKin13 regulates depolymerization of cytoplasmic...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (16): 3904–3913.
Published: 15 August 2012
..., FAP46, FAP74 and FAP221/Pcdp1, which exhibits Ca 2+ -sensitive calmodulin binding. Here we report the isolation and characterization of a Chlamydomonas reinhardtii null mutant for FAP46. This mutant, fap46-1 , lacks the C1d projection and has impaired motility, confirming the importance...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2012) 125 (14): 3357–3366.
Published: 15 July 2012
... pair of microtubules is nucleated or how their associated polypeptides are assembled. Here we report that the Chlamydomonas pf19 mutation results in a single amino acid change within the p60 catalytic subunit of katanin, and that this mutation prevents microtubule severing activity. The pf19 mutant has...
Includes: Supplementary data
Journal Articles
J Cell Sci (2012) 125 (12): 2885–2895.
Published: 15 June 2012
...Karin Komsic-Buchmann; Lisa Marie Stephan; Burkhard Becker Summary Contractile vacuoles (CVs) are essential for osmoregulation in many protists. To investigate the mechanism of CV function in Chlamydomonas , we isolated novel osmoregulatory mutants. Four of the isolated mutant cell lines carried...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2007) 120 (20): 3653–3665.
Published: 15 October 2007
.... Retrograde IFT requires cytoplasmic dynein 1b, a motor complex whose organization, structural composition and regulation is poorly understood. We have characterized the product of the Chlamydomonas FAP133 gene that encodes a new WD-repeat protein similar to dynein intermediate chains and homologous...
Journal Articles
J Cell Sci (2007) 120 (5): 858–867.
Published: 1 March 2007
... tissues. Moreover, a NudC ortholog is upregulated upon deflagellation in Chlamydomonas. We found that mammalian Lis1 localizes to motile cilia in trachea and oviduct, but is absent from non-motile primary cilia. Furthermore, we cloned a gene encoding a Lis1-like protein (CrLis1) from Chlamydomonas...
Journal Articles
J Cell Sci (2005) 118 (20): 4655–4665.
Published: 15 October 2005
...Matthew J. Wargo; Erin E. Dymek; Elizabeth F. Smith Studies of flagellar motility in Chlamydomonas mutants lacking specific central apparatus components have supported the hypothesis that the inherent asymmetry of this structure provides important spatial cues for asymmetric regulation of dynein...
Includes: Supplementary data
Journal Articles
J Cell Sci (2005) 118 (3): 529–537.
Published: 1 February 2005
...Noriko Okita; Nahoko Isogai; Masafumi Hirono; Ritsu Kamiya; Kenjiro Yoshimura In the mechanism underlying the phototactic behavior of Chlamydomonas , Ca 2+ has been thought to control the dominance between the two flagella so as to steer the cell to correct directions. A newly isolated mutant, lsp1...
Journal Articles
J Cell Sci (2004) 117 (18): 4179–4188.
Published: 15 August 2004
...Hui Zhang; David R. Mitchell Mutations at CPC1 disrupt assembly of a central pair microtubule-associated complex and alter flagellar beat frequency in Chlamydomonas . Sequences of wild-type genomic clones that complement cpc1 , and of corresponding cDNAs, reveal the gene product to be a 205 kDa...
Journal Articles
J Cell Sci (2004) 117 (12): 2533–2544.
Published: 15 May 2004
... conducted using high Ca 2+ conditions. In Chlamydomonas , high Ca 2+ induces changes in waveform which are predicted to result from regulating dynein activity on specific microtubules. Our results demonstrate that microtubule sliding in high Ca 2+ buffer is also induced by dynein arms on specific doublets...