... are involved in NE maintenance, but the underlying mechanism remains unclear. Here, we found that the ceramide synthase (CerS) homolog in the fission yeast Schizosaccharomyces pombe Tlc4 (SPAC17A2.02c) suppressed NE defects in cells lacking the NE proteins Lem2 and Bqt4. Tlc4 possesses a TRAM/LAG1/CLN8 domain...

...Asmahan Abu-Arish; Elvis Pandžić; Yishan Luo; Yukiko Sato; Mark J. Turner; Paul W. Wiseman; John W. Hanrahan ABSTRACT Membrane proteins often cluster in nanoscale membrane domains (lipid rafts) that coalesce into ceramide-rich platforms during cell stress, however the clustering mechanisms remain...

... death Ceramide Mitophagy European Research Council 10.13039/100010663 322709 Autophagy is a regulated, catabolic process in which double-membrane vesicles, called autophagosomes, are formed de novo to engulf cytoplasmic content, which is then degraded upon fusion...

.... Thus, Cwh43 affects utilization of glucose and nitrogen sources, as well as storage lipid metabolism. These results may fit a notion developed in budding yeast stating that Cwh43 conjugates ceramide to glycosylphosphatidylinositol (GPI)-anchored proteins and maintains integrity of membrane organization...

... that acid sphingomyelinase (ASM; also known as SMPD1) regulates the conserved DAF-2 (the ortholog IGF-1R in mammals) RTK signaling pathway in Caenorhabditis elegans . How ASM and its catalytic products, ceramides, control RTK signaling pathways remain unclear. Here, we report that ASM regulates...

...-to-Golgi transport is crucial for maintaining the different membrane lipid composition and identities of organelles. Despite their importance, mechanisms regulating transport remain elusive. Here we report that in yeast coat protein complex II (COPII) vesicle-mediated transport of ceramide from the ER...

...Esther Marza; Karina T. Simonsen; Nils J. Færgeman; Giovanni M. Lesa Glycosphingolipids (GSLs) are glycosylated derivatives of ceramide in the lipid bilayer. Their ubiquitous distribution and complexity suggest that they have important functions, but what these are in vivo is still poorly...

... in ceramide-induced mitochondrial apoptosis. Ceramide induced GSK-3β activation via protein dephosphorylation at serine 9. We previously reported that ceramide induced caspase-2 and caspase-8 activation, Bid cleavage, mitochondrial damage, and apoptosis. In this study, we found that caspase-2 activation...

...Saïda Mebarek; Hiba Komati; Fabio Naro; Caroline Zeiller; Monica Alvisi; Michel Lagarde; Annie-France Prigent; Georges Némoz In L6 skeletal myoblasts induced to differentiate by Arg8-vasopressin treatment, a short-lived lowering of ceramide levels was observed, followed by a long-lasting...

... and/or protein factors, thereby supporting the physiological Ca 2+ sensitivity of membrane fusion. Furthermore, the sphingomyelinase-driven generation of ceramide, contributing to the total membrane negative curvature, preserves the ability to fuse despite extensive cholesterol removal. Membrane microdomain...

..., including smooth muscle and neurones. The mechanisms regulating the surface expression have however not yet been elucidated. Here we show, using stable HERG-expressing HEK 293 cells, that ceramide evokes a time-dependent decrease in HERG current which was not attributable to a change in gating properties...

... of cytosolic oligonucleosomes and by TUNEL assay to visualize DNA fragmentation. Nerve growth factor also induced a number of cell-death-related intracellular events including ceramide generation, caspase activation and poly-(ADP ribose)polymerase cleavage. Again, p75 receptor blockade completely abolished all...

...Claudio A. Hetz; Martin Hunn; Patricio Rojas; Vicente Torres; Lisette Leyton; Andrew F. G. Quest Engagement of the Fas receptor promotes apoptosis by activation of caspases. In addition, alterations in plasma membrane lipid orientation and intracellular ceramide levels are often observed. In A20 B...

... in the mitochondria. It also inhibited glucose deprivation-induced cytochrome c release from the mitochondria. It is possible that overexpression of Bcl2 prevents glucose deprivation-induced ceramide generation, probably by preventing the leakage of hydroperoxide from the mitochondria. We also observed that glucose...

...Takuya Fukunaga; Masami Nagahama; Kiyotaka Hatsuzawa; Katsuko Tani; Akitsugu Yamamoto; Mitsuo Tagaya ABSTRACT We examined the effects of short chain and long chain ceramides on the stability of the Golgi apparatus. Short chain ceramides, C 2 - and C 6 -ceramides, blocked brefeldin A-induced Golgi...

... changes, DNA laddering patterns, and DNA fragmentation associated with apoptosis. Elevation of ceramide with exogenous ceramide analogs was sufficient for apoptosis induction with the same characteristics and in the same time frame. H 2 O 2 induced rapid sphingomyelin hydrolysis to ceramide, the elevation...

... of GPI-anchored proteins before arrival to the Golgi. The end8-1/ lcb1-100 mutant is defective in ceramide synthesis. In vitro data suggest a requirement for ceramides after the exit from the ER. We therefore propose that ceramides might function in the fusion of a GPI-containing vesicle with the Golgi...