...Ryoko Kuriyama; Cody R. Fisher ABSTRACT The centrosome, which consists of centrioles and pericentriolar material (PCM), becomes mature and assembles mitotic spindles by increasing the number of microtubules (MTs) emanating from the PCM. Among the molecules involved in centrosome maturation, Cep192...

...Divya Ganapathi Sankaran; Alexander J. Stemm-Wolf; Bailey L. McCurdy; Bharath Hariharan; Chad G. Pearson ABSTRACT Microtubules (MTs) promote important cellular functions including migration, intracellular trafficking, and chromosome segregation. The centrosome, comprised of two centrioles...

... . 10.1038/nature01601 Sharp , D. J. , Yu , K. R. , Sisson , J. C. , Sullivan , W. and Scholey , J. M. ( 1999 ). Antagonistic microtubule-sliding motors position mitotic centrosomes in Drosophila early embryos . Nat. Cell Biol.   1 , 51 - 54 . 10.1038/9025 Sharp...

... that need instant attention. Examples are various types of DNA lesion but also the presence of extra centrosomes that can foster aneuploidy and, ultimately, promote DNA damage. Here, we review the role of PIDD1 in response to DNA damage and also highlight novel functions of PIDD1, such as in centrosome...

...Sascha Werner; Ana Pimenta-Marques; Mónica Bettencourt-Dias ABSTRACT Centrosomes and cilia are present in organisms from all branches of the eukaryotic tree of life. These structures are composed of microtubules and various other proteins, and are required for a plethora of cell processes...

... ciliogenesis. Cilia Satellites Centrosomes Basal body SSX2IP WDR8 WRAP73 Cep135 The centrosome is the main microtubule-organising centre (MTOC) of most animal cells. Centrosomes generally consist of two orthogonally arranged centrioles surrounded by layers of pericentriolar material (PCM...

... inner and outer centriole components. * Author for correspondence ( [email protected] ) 6 8 2012 © 2012. Published by The Company of Biologists Ltd 2012 Bld10 Cell biology Centrioles Centrosomes Cep135 Drosophila Centrioles are complex MT-based structures...

... to cancer progression. β-Catenin is also a centrosomal component involved in centrosome separation. Centrosomes nucleate interphase microtubules and the bipolar mitotic spindle in normal cells, but their organization and function in human cancers are abnormal. Here, we show that expression of stabilized...

...Rebecca L. Ferguson; Gaetan Pascreau; James L. Maller Centrosomes are the major microtubule-organizing centers in animal cells and regulate formation of a bipolar mitotic spindle. Aberrant centrosome number causes chromosome mis-segregation, and has been implicated in genomic instability and tumor...

... undergo further mitoses) and cyst cell daughters (which withdraw from the cell cycle and become quiescent). Although a considerable body of evidence indicates important roles for centrosomes in spindle orientation and asymmetrical division of GSCs, the behaviour and function of the centrioles in CPCs...

...Ryoko Kuriyama; Monica Bettencourt-Dias; Ingrid Hoffmann; Marc Arnold; Lisa Sandvig Cancer cells frequently induce aberrant centrosomes, which have been implicated in cancer initiation and progression. Human colorectal cancer cells, HCT116, contain aberrant centrioles composed of disorganized...

... null mutation. NudE can associate with kinetochores, spindles and the nuclear envelope. In nudE mutant brain mitotic cells, centrosomes are often detached from the poles. Moreover, the centrosomes of mutant primary spermatocytes do not migrate from the cell cortex to the nuclear envelope, establishing...

...Ryoko Kuriyama; Yasuhiko Terada; Kyung S. Lee; Christopher L. C. Wang Centrosome duplication is tightly coupled with the cell cycle and neither too many nor too few centrosomes are induced in a normal cell. To study how centrosome assembly is regulated, we analyzed the abnormal process of multiple...

...Timothy L. Megraw; Sandhya Kilaru; F. Rudolf Turner; Thomas C. Kaufman The Drosophila Centrosomin (Cnn) protein is an essential core component of centrosomes in the early embryo. We have expressed a Cnn-GFP fusion construct in cleavage stage embryos, which rescues the maternal effect lethality...

... and in the centrosomal region, where it co-localizes withγ-tubulin. In Huntington's disease, inclusions are often nuclear or perinuclear. Since the perinuclear concentration of huntingtin does not depend on the number of its glutamine repeats, we propose that inclusions are found in perinuclear and intranuclear...

... molecular mass as in Drosophila (Rb188 a 185 kDa antigen and Bx63 185 kDa and 66 kDa antigens). By immunocytochemistry we show that the centrosomes of Parascaris con-tain the 185 kDa antigen recognized by polyclonal Rb188 and monoclonal Bx63 antibodies. In addition, Bx63 reveals cytoplasmic midzone...

...Roger J. Leslie ABSTRACT To examine the relative roles of chromosomes, spindle poles and microtubules in the formation of the metaphase spindle and metakinesis, I have experimentally placed an extra centrosome-free pronucleus close to a forming bipolar spindle in a living cell. The chromosomes from...

...Markku Kallajoki; Klaus Weber; Mary Osborn ABSTRACT The SPN antigen plays an essential role in mitosis, since microinjection of antibodies causes mitotic arrest. Here we show, by examination of the relative locations of SPN antigen, the centrosomal 5051 antigen and tubulin in normal mitotic...

...Takami Maekawa; Ryoko Kuriyama ABSTRACT As cells enter mitosis, centrosomes undergo many transformations and become associated with different molecules in a stage-specific manner. We have developed a protocol for immunofluorescence staining with four antibody probes that can help us to follow...

...Giuliano Callaini; Maria Giovanna Riparbelli ABSTRACT Centriole and centrosome cycles were examined by indirect immunofluorescence and electron microscopy techniques in the early Drosophila embryo. The centrosomes, which are already divided at interphase, appear as compact spheres during prophase...