...Andrew P. Porter; Hannah Reed; Gavin R. M. White; Erinn-Lee Ogg; Helen J. Whalley; Angeliki Malliri ABSTRACT Centriole duplication is tightly controlled to maintain correct centriole number through the cell cycle. Key to this is the regulated degradation of PLK4, the master regulator of centriole...

... partner. RACK1, a scaffold protein that interacts with multiple proteins through its seven WD40 domains, directly binds to BRCA1 and localizes to centrosomes. RACK1 knockdown suppresses centriole duplication, whereas RACK1 overexpression causes centriole overduplication in a subset of mammary gland...

... proteins, but not either one, blocks loading of the Cep63–Cep152 complex to mother centrioles and consequently prevents centriole duplication. We propose that Cep57 and Cep57l1 function redundantly to ensure recruitment of the Cep63–Cep152 complex to the mother centrioles for procentriole formation...

... is therefore crucial for genome stability and embryogenesis. Centriole duplication requires the serine/threonine protein kinase Plk4. Here, we identify Cep78 as a human centrosomal protein and a new interaction partner of Plk4. Cep78 is mainly a centriolar protein that localizes to the centriolar wall...

...Ching-Wen Chang; Wen-Bin Hsu; Jhih-Jie Tsai; Chieh-Ju C. Tang; Tang K. Tang ABSTRACT Centriole duplication is a tightly ordered process during which procentrioles are assembled in G1-S and elongate during S and G2. Here, we show that human CEP295 ( Drosophila Ana1) is not essential for initial...

...Marco Gottardo; Giuliano Callaini; Maria Giovanna Riparbelli ABSTRACT Disengagement of parent centrioles represents the licensing process to restrict centriole duplication exactly once during the cell cycle. However, we provide compelling evidence that this general rule is overridden in insect...

... to ensure that centriole duplication occurs once per cell cycle. Here, we report that phosphatidylinositol 4-phosphate 5-kinase type-1 gamma (PIP5K1C, hereafter referred to as PIPKIγ) plays an important role in centriole fidelity. PIPKIγ localized in a ring-like pattern in the intermediate pericentriolar...

...Katharina F. Sonnen; Anna-Maria Gabryjonczyk; Eduard Anselm; York-Dieter Stierhof; Erich A. Nigg Summary Polo-like kinase 4 (Plk4) is a key regulator of centriole duplication, but the mechanism underlying its recruitment to mammalian centrioles is not understood. In flies, Plk4 recruitment depends...

...Christian Arquint; Katharina F. Sonnen; York-Dieter Stierhof; Erich A. Nigg Control of centriole number is crucial for genome stability and ciliogenesis. Here, we characterize the role of human STIL, a protein that displays distant sequence similarity to the centriole duplication factors Ana2...

...Ramona Bahtz; Joerg Seidler; Marc Arnold; Uta Haselmann-Weiss; Claude Antony; Wolf D. Lehmann; Ingrid Hoffmann Centriole duplication occurs once per cell cycle and requires Plk4, a member of the Polo-like kinase family. A key component of the centrosome is the γ-tubulin ring complex (γ-TuRC...

... during mitosis. In this study, we observed that centriole duplication was inhibited in NEK7-depleted cells. Ectopic expression of centrosome-directed NEK7 led to the formation of extra centrioles in a kinase-activity-dependent manner. We also observed extra centriole formation in centrosome-directed NEK6...

... Autophosphorylation βTrCP Centriole duplication Centrioles are the main constituents of the mammalian centrosome and act as basal bodies for ciliogenesis ( Nigg and Raff, 2009 ). Centrosomes organize the cytoplasmic microtubule network during interphase and the mitotic spindle during mitosis...