... regarding the physical division of primary spermatocytes during meiosis I. Here, we investigated this process using high-resolution time-lapse confocal microscopy and examined the spatiotemporal regulation of contractile ring assembly in C. elegans primary spermatocytes. We found that centralspindlin...

...Elaina B. Breznau; Megan Murt; T. Lynne Blasius; Kristen J. Verhey; Ann L. Miller ABSTRACT Centralspindlin, a complex of the kinesin-6-family member MKLP1 and MgcRacGAP (also known as Kif23 and Racgap1, respectively), is required for cytokinesis and cell–cell junctions. During anaphase...

...Eri Asano; Hitoki Hasegawa; Toshinori Hyodo; Satoko Ito; Masao Maeda; Masahide Takahashi; Michinari Hamaguchi; Takeshi Senga Summary Centralspindlin, which is composed of MgcRacGAP and MKLP1, is essential for central spindle formation and cytokinetic furrow ingression. MgcRacGAP utilizes its GAP...

... microtubules are regulated in a time- and region-dependent manner. Here, we show that α-actinin-regulated cortical actin filament integrity is crucial to specify different populations of microtubules during cell division in mammalian cells. Depletion of α-actinin caused aberrant recruitment of centralspindlin...

... of the actomyosin contractile ring. We employed affinity-purification methodology coupled with mass spectrometry to identify Anillin-interacting molecules in Drosophila cells. We isolated several actin and myosin proteins, three of the five Drosophila septins and RacGAP50C (Tum), a component of the centralspindlin...

... mutants of the centralspindlin component ZEN-4, prevents completion of cytokinesis but does not inhibit furrowing. However, furrowing is inhibited by the simultaneous depletion of ZEN-4 with either PAR-2 or Gα, which are required for asymmetric divisions. Through studies of other genes required...

.... Here we show that in Drosophila depletion of either the GAP (GTPase-activating protein) or the kinesin-like subunit of the evolutionary conserved centralspindlin complex prevents furrowing without affecting the association of astral microtubules with the cell cortex. Moreover, time-lapse imaging...

... astral and central spindle microtubules can recruit RhoA at the equatorial cortex. We find that centralspindlin and ECT2 are required for RhoA localization and furrowing. Centralspindlin is localized both to central spindle microtubules and at the tips of astral microtubules near the equatorial cortex...

[email protected]) 17 8 2005 © The Company of Biologists Limited 2005 2005 Cytokinesis RacGAP Drosophila Rho-GTPase signalling Centralspindlin GTPase-activating protein After chromosomes have moved to the poles of the mitotic spindle in a dividing cell, a complex set of cellular...