...Celia Municio-Diaz; Elise Muller; Stéphanie Drevensek; Antoine Fruleux; Enrico Lorenzetti; Arezki Boudaoud; Nicolas Minc ABSTRACT The cell wall (CW) is a thin and rigid layer encasing the membrane of all plant and fungal cells. It ensures mechanical integrity by bearing mechanical stresses derived...

...: A multiscale review on leaf morphogenesis, focusing on coordinated, non-cell-autonomous growth regulations via metabolism and biomechanics. Biomechanics Cell wall Leaf morphogenesis Metabolism Mechanical feedback Proprioception Japan Society for the Promotion of Science http://dx.doi.org...

... and shifts the balance between its roles in promoting brassinosteroid and phytosulfokine signalling, respectively. Brassinosteroid Cell wall Endomembrane system Phosphorylation Trafficking Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659 WO 1660/6-1...

... with strengthened lateral cell walls and protruding bud scars. The latter result from aberrant cytokinesis, as observed by following the budding process in vivo and by transmission electron microscopy of the bud neck region. This phenotype can be suppressed by KlCDC42 G12V , which encodes a hyper-active variant...

...Yuko Sukegawa; Takahiro Negishi; Yo Kikuchi; Keiko Ishii; Miyuki Imanari; Farzan Ghanegolmohammadi; Satoru Nogami; Yoshikazu Ohya ABSTRACT The cell wall integrity checkpoint monitors synthesis of cell wall materials during the Saccharomyces cerevisiae cell cycle. Upon perturbation of cell wall...

...Edwin R. Lampugnani; Ghazanfar Abbas Khan; Marc Somssich; Staffan Persson; Jenny Russinova ABSTRACT Plant cells are surrounded by a strong polysaccharide-rich cell wall that aids in determining the overall form, growth and development of the plant body. Indeed, the unique shapes of the 40-odd cell...

... for safe gradual cell abscission . J. Cell Biol.   198 , 637 - 656 . 10.1083/jcb.201202015 de Leon , N. , Sharifmoghadam , M. R. , Hoya , M. , Curto , M.-A. , Doncel , C. and Valdivieso , M.-H. ( 2013 ). Regulation of cell wall synthesis by the clathrin light chain...

... Cell wall Chitin Glucan Morphogenesis is an essential process during development in all organisms. It must be under strict control to produce precise shapes and reproduce them indefinitely. Despite its importance, little is known about its regulation at the molecular level. For many years, we...

... by cortical microtubules: a review and a model . Protoplasma 215 , 150 - 171 . Baskin T. I. ( 2005 ). Anisotropic expansion of the plant cell wall . Annu. Rev. Cell Dev. Biol. 21 , 203 - 222 . Busby C. H. , Gunning B. E. S. ( 1983 ). Orientation ofmicrotubules against...

... and moulds, and both cellular forms have been studied extensively as tractable models of cell polarity. Chitin is a hallmark component of fungal cell walls. Chitin synthesis is essential for growth, viability and rescue from many conditions that impair cell-wall integrity. In the polymorphic human pathogen...

... that of a cell surface receptor not associated with the cell wall, is influenced by the presence of fucose side chains on one or more unidentified molecules that might include pectin, and is dependent upon cellulose synthesis on the plasma membrane. WAK is crosslinked into a detergent-insoluble complex within...

... to be a part of the GS enzyme and essential for maintaining cell integrity during cytokinesis and polarized growth. Here we show that bgs4 + , cwg1 + ( cwg1-1 shows reduced cell-wall β-glucan and GS catalytic activity) and orb11 + ( orb11-59 is defective in cell morphogenesis) are the same gene. bgs4...

... , suggesting Bgs1p is involved in septum cell-wall (1,3)β-D-glucan synthesis at cytokinesis. We have studied the in vivo physiological localization of Bgs1p in a bgs1 Δ strain containing a functional GFP-bgs1 + gene (integrated single copy and expressed under its own promoter). During vegetative growth, Bgs1p...

...Jose M. Rodriguez-Peña; Cristina Rodriguez; Alberto Alvarez; César Nombela; Javier Arroyo The cell wall is an essential structure that preserves the osmotic integrity of fungal cells and determines cellular morphology during developmental programs. The high number of different wall components...

... and to a lesser extent at the septum. Mok1 is delocalised and upregulated in myo52 Δ and myo52 Δ cell walls are resistant to digestion by the cell wall degrading enzyme zymolyase. Thus myo52 + appears to be involved in the local delivery or positioning of vesicles containing cell wall precursors at the cell tips...

... in cell wall biosynthesis and cytoskeletal polarity, is structurally and functionally related to the Rho- associated kinases (PRK/ROCK) of mammalian cells. In this study, localization of Pkc1p was monitored in live cells using a GFP fusion (Pkc1p-GFP). Pkc1p-GFP showed dynamic spatial and temporal...

... Laboratory, University of California, San Francisco, CA 94143-0868 USA 27 09 1999 17 11 1999 © 1999 by Company of Biologists 1999 Cellulose-binding protein PsB Cell wall Extracellular matrix The spore coat of Dictyostelium discoideum is a 210 nm-thick layered...

... that pck2p is more important in the regulation of the enzyme (1-3)β-D-glucan synthase. Thus, overexpression of pck2 + , but not pck1 + , caused a general increase in cell wall biosynthesis, mainly in β-glucan, and (1-3)β-D-glucan synthase activity was considerably augmented. When this activity was separated...

...Adrian Turner; Brian Wells; Keith Roberts ABSTRACT A procedure is described for obtaining clean maize cell wall preparations that contain embedded plasmodesmata. Negative staining and rotary shadowing have been used with transmission electron microscopy to visualise the plasmodesmata...

... in both the composition and the orientation of cell wall polymers. We have obtained infor-mation on the orientation of wall polymers in elongating cells by two complementary techniques, one using microscopy and one spectroscopy. Images obtained by the fast-freeze, deep-etch, rotary-shadowed replica tech...