... describe how a direct interaction between the Rho GTPase Cdc42 and the GTPase-activating protein (GAP) GRAF1 (also known as ARHGAP26), facilitates rapid cell surface turnover at the leading edge. Both Cdc42 and GRAF1 were required for fluid-phase uptake and regulated the generation of transient GRAF1...

...Jürgen J. Heinisch; Peter N. Lipke; Audrey Beaussart; Sofiane El Kirat Chatel; Vincent Dupres; David Alsteens; Yves F. Dufrêne Summary Living cells use cell surface proteins, such as mechanosensors, to constantly sense and respond to their environment. However, the way in which these proteins...

... in glycoprotein biosynthesis; however, a small portion of GalT I is expressed on the cell surface where it functions as a matrix receptor by binding terminal N -acetylglucosamine residues on extracellular glycoside ligands. The GalT I polypeptide occurs in two alternate forms that differ only in the length...

... to the cell surface through the trans-Golgi network and endosomal system, and its only known role on plasma membrane consists in activation of opportunistic pathogenic entities. Here, we report findings about the interaction of furin with MT1-MMP and the integrinαV at the cell surface. By using in vivo gene...

... physiological conditions in the near future. *Author for correspondence (e-mail: [email protected] ) © The Company of Biologists Limited 2001 2001 Super-resolution Near-field scanning optical microscopy Single-molecule detection Distribution Cell surface Membrane GFP...

... glycosyltransferases, as well as on the cell surface, where it functions as a signaling receptor for extracellular glycoside ligands. When expressed on the surface, GalT associates with the cytoskeleton and, upon ligand-induced aggregation, induces cell-type specific intracellular signal cascades. In an effort...

... of highly metastatic murine and human cell lines are characterized by elevated levels of cell surface GalT I, although the intracellular biosynthetic pool is similar between cells of high and low metastatic potential. In this study, we examined whether the elevated expression of surface GalT I...

... initiates a contact inhibition response in colliding cells. * Author for correspondence 06 02 1996 03 07 1996 © 1996 by Company of Biologists 1996 Contact inhibition Cell motility Cytoskeleton Cell surface Plasma membrane Phospholipase Contact inhibition...

... to enter the lipid-anchored clusters enables it to activate the signalling pathways that normal Thy-1 uses. Thus the membrane anchor, in targetting Thy-1 to different microdomains on the cell surface, determines its ability to inhibit neurite outgrowth on astrocytes. * Author for correspondence...

... used the chemical technique, hydrazinolysis, to release oligosaccharides from cell surface glycoproteins of Dictyostelium discoideum . Oligomannose type, xylose- and fucose-containing oligosaccharides were found to be present. The charged oligosaccharides contained sulphate and mannose 6-phosphate...

...Bruce F. Holifield; Ken Jacobson ABSTRACT Patches of aggregated membrane proteins on motile fibroblasts are transported from the surfaces of the leading lamella to a site just ahead of the nucleus in the phenomenon known as capping. A major cell surface glycoprotein, Pgp-1 (GP80), was tagged...

... of Biologists 1990 calcium flagella membrane glycoprotein Chlamydomonas signal transduction cell surface Eukaryotic cells exercise control over the distribution of plasma membrane proteins; they can maintain asymmetric distributions of cell surface proteins and can actively change...

... Minnesota, pp. 121 – 137 . Estrada-Garcia , M. T. , Green , J. R. , Booth , J. M. , White , J. G. and Callow , J. A. ( 1989 ). Monoclonal antibodies to cell surface components of zoospores and cysts of the fungus Pythium aphanidennatum reveal species-specific antigens . Expl...

...Kazushi Fujimoto; Pedro Pinto Da Silva ABSTRACT We report here the macromolecular dynamics of the cell surface of rat alveolar macrophages during spreading on a substratum, a process that involves the formation of numerous coated pits. We used ‘fracture-flip’ to prepare high-resolution platinum...

... embedding box. F.G. gratefully acknowledges the receipt of a National Research Fellowship from the Australian Government. REFERENCES Antakly , T. ( 1984 ). The cell surface during the secretory process . In Cell Biology of the Secretory Process (ed. M. Cantin ), pp. 171 – 195...

... and tightly coupled to the underlying cytoskeleton (axoneme). Because of its cell surface orientation and axonemal linkage, it is likely that the 240K glycoprotein plays an important role in the adhesive and/or motile phenomena exhibited by the C. moewusii flagellar surface. * Author for correspondence...

... were processed for electron microscopy and micrographs were digitized for computerized analysis: the cell surface curvature (at the micrometre level) and rugosity (at the submicrometre level) were quantified in free and adhesion-involved regions. Also, the size of cell interaction areas...

... with the cell surface. Only glass particles stuck to the surface move backwards, whereas the others follow the general forward flow described before. Under a sufficiently large fragment of glass, as shown in Fig. 2 , the development of an adhesive knob is observed, after which the particle starts to move back...

... of a single 68 000Mr glycoprotein. * Author for correspondence. 02 07 1985 13 11 1985 © 1986 by Company of Biologists 1986 human spermatozoa cell surface glycoproteins detergent resistance Cell surface components are interesting, because they are important in various...

...Paul T. Sharpe; D. J. Watts ABSTRACT Changes in the cell surface properties of amoebae of Dictyostelium discoideum during growth in different culture conditions have been studied by aqueous two-phase partitioning on a thin-layer countercurrent distribution apparatus. Changes in cell surface...