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Keywords: Cell spreading
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Journal Articles
J Cell Sci (2020) 133 (5): jcs236968.
Published: 06 March 2020
... pseudopodia during phagocytosis. This is consistent with a role for intact ezrin in maintaining folded membrane on the cell surface, which then becomes available for cell spreading and phagocytosis. The principle of the method using two using two fluorescent protein tags relies on monitoring the separation...
Journal Articles
J Cell Sci (2019) 132 (10): jcs224030.
Published: 15 May 2019
... that following detachment and replating, CLCa is uniquely responsible for promoting efficient cell spreading and migration. Selective depletion of CLCa, but not of CLCb, reduced the initial phase of isotropic spreading of HeLa, H1299 and HEK293 cells by 60–80% compared to siRNA controls, and wound closure...
Includes: Supplementary data
Journal Articles
J Cell Sci (2013) 126 (20): 4627–4635.
Published: 15 October 2013
..., no new membrane is added into the plasma membrane. Pharmacological inhibition of calpain-activation, which is triggered by Ca 2+ influx during neutrophil spreading, prevents normal cell flattening. In calpain-suppressed cells, an aberrant form of cell spreading can occur where an uncoordinated...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (16): 2702–2710.
Published: 15 August 2011
... contributed equally to this work ‡ Author for correspondence ( martin_hemler@dfci.harvard.edu ) 1 4 2011 © 2011. 2011 CD9 Tetraspanin SILAC Microvilli Cell adhesion Cell spreading The tetraspanin protein family contains 33 distinct members, each with four transmembrane...
Includes: Supplementary data
Journal Articles
J Cell Sci (2011) 124 (11): 1808–1818.
Published: 01 June 2011
...-signal transduction, we performed a small-scale siRNA screen in A549 lung epithelial cells. Of the Rap effectors tested, only Radil blocked Rap-induced spreading. Additionally, we identified a novel role for Ezrin downstream of Rap1. Ezrin was necessary for Rap-induced cell spreading, but not Rap-induced...
Includes: Supplementary data
Journal Articles
J Cell Sci (2010) 123 (22): 3901–3912.
Published: 15 November 2010
... interaction by overexpressing the minimal kinectin–kinesin interaction domain on kinectin in cells. This perturbation resulted in a morphological change to a rounded cell shape and reduced cell spreading and migration. Immunofluorescence and live-cell imaging demonstrated a kinectin-dependent ER extension...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2010) 123 (3): 413–423.
Published: 01 February 2010
... irrespective of the nature of adhesive contacts. When NMII activity is inhibited during cell spreading by Rho kinase inhibition, blebbistatin, caldesmon overexpression or NMIIA RNAi, the symmetric traction forces are lost and cell spreading persists, causing cytoplasm fragmentation by membrane tension...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2009) 122 (10): 1637–1646.
Published: 15 May 2009
... in the regulation of cell spreading and directional migration in response to various stimuli. Here we show that discoidin domain receptor 1 (DDR1), a tyrosine kinase receptor activated by type I collagen, associates with the non-muscle myosin IIA heavy chain (NMHC-IIA) upon ligand stimulation. An association...
Includes: Supplementary data
Journal Articles
J Cell Sci (2008) 121 (18): 3071–3082.
Published: 15 September 2008
... PxxP motifs, which bind Src homology 3 (SH3) domains, are indispensable for the coordinated regulation of filopodium and focal adhesion formation and cell-spreading dynamics during attachment. Candidate Abl PxxP-motif-binding partners were identified by screening a comprehensive SH3-domain phage...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2008) 121 (5): 695–705.
Published: 01 March 2008
..., impaired cell-cell adhesion, enhanced cell spreading and an increase of β1 integrin activity–effects similar to those of constitutively active R-Ras(38V). Overexpression of ORP3 leads to formation of polarized cell-surface protrusions, impaired cell spreading and decreased β1 integrin activity. In primary...
Includes: Supplementary data
Journal Articles
J Cell Sci (2007) 120 (22): 3989–3998.
Published: 15 November 2007
... The Company of Biologists Limited 2007 2007 Lsc p115 RhoGEF Leukemia-associated RhoGEF RhoA Fibronectin Guanine nucleotide exchange factors Extracellular matrix Stress fibers Focal adhesions Cell spreading The ability of most cells to survive, proliferate and migrate is regulated...
Journal Articles
J Cell Sci (2007) 120 (21): 3792–3803.
Published: 01 November 2007
... to the subfragment 2 domains of nonmuscle myosin IIA and myosin IIB and to the N-terminus of the long form of myosin light chain kinase (L-MLCK). SV inhibits cell spreading via an MLCK- and myosin II-dependent mechanism. Overexpression of SV reduces the rate of cell spreading, and RNAi-mediated knockdown...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2007) 120 (5): 802–814.
Published: 01 March 2007
...Marko Jović; Naava Naslavsky; Debora Rapaport; Mia Horowitz; Steve Caplan β1 integrins bind to the extracellular matrix and stimulate signaling pathways leading to crucial cellular functions, including proliferation, apoptosis, cell spreading and migration. Consequently, control of β1 integrin...
Journal Articles
J Cell Sci (2007) 120 (3): 395–406.
Published: 01 February 2007
... of vinculin on tyrosine residues 100 and 1065 , mediated by SRC kinases, affects cell spreading. Mol. Biol. Cell 15 , 4234 -4247. This study was supported by a Deutsche Forschungsgemeinschaft (DFG) SFB628 grant to R.T. We would like to thank Ivan Dikic (Frankfurt) for the generous gift of Src...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2007) 120 (1): 66–76.
Published: 01 January 2007
...Coert Margadant; Angelique van Opstal; Johannes Boonstra Prevention of cell spreading or disruption of actin filaments inhibits growth factor stimulated cell cycle re-entry from quiescence, mainly because of a failure to induce cyclin D expression. Ectopic cyclin D expression overrules anchorage...
Journal Articles
J Cell Sci (2006) 119 (7): 1307–1319.
Published: 01 April 2006
...Aude S. Ada-Nguema; Harry Xenias; Michael P. Sheetz; Patricia J. Keely R-Ras, an atypical member of the Ras subfamily of small GTPases, enhances integrin-mediated adhesion and signaling through a poorly understood mechanism. Dynamic analysis of cell spreading by total internal reflection...
Includes: Multimedia, Supplementary data
Journal Articles
J Cell Sci (2005) 118 (2): 381–395.
Published: 15 January 2005
...Katarzyna Bialkowska; Takaomi C. Saido; Joan E. B. Fox In this study, we used cultured cells spreading on β3 integrin substrates to examine the possibility that spectrin is involved in signal transduction. Spectrin clustered with specialized calpain-induced β3 integrin signaling complexes...
Journal Articles
J Cell Sci (2004) 117 (14): 2951–2961.
Published: 15 June 2004
... and the cortical actin cytoskeleton independent of receptor activity. * Author for correspondence (e-mail: ramesh@helix.mgh.harvard.edu ) 12 2 2004 © The Company of Biologists Limited 2004 2004 βPDGFR NHERF Cell spreading Migration Merlin ERM The Na + /H + exchanger regulatory...
Journal Articles
J Cell Sci (2004) 117 (7): 1259–1268.
Published: 01 March 2004
..., King's College London, London SE1 1UL, UK ‡ These authors contributed equally to the work 10 11 2003 © The Company of Biologists Limited 2004 2004 Rac1 Cell spreading GTPase Macrophage To elucidate the expression profile of Rac isoforms in CSF-1-dependent bone...
Journal Articles
J Cell Sci (2003) 116 (8): 1617–1625.
Published: 15 April 2003
... actin polymerization that drives extension of the leading cell edge. Subsequently, RhoA regulates myosin-dependent contractile force,which is required for formation of adhesive contacts and stress fibers. During cell spreading, however, the activity of RhoA is reduced by a mechanism involving...