...Linda Clijsters; Rob Wolthuis ABSTRACT Cdc6 and Cdt1 initiate DNA replication licensing when cells exit mitosis. In cycling cells, Cdc6 is efficiently degraded from anaphase onwards as a result of APC/C–Cdh1 activity. When APC/C–Cdh1 is switched off again, at the end of G1 phase, Cdc6 could thus re...

...Vassilis Roukos; Ali Kinkhabwala; Julien Colombelli; Panagiotis Kotsantis; Stavros Taraviras; Hideo Nishitani; Ernst Stelzer; Philippe Bastiaens; Zoi Lygerou For genomic integrity to be maintained, the cell cycle and DNA damage responses must be linked. Cdt1, a G1-specific cell-cycle factor...

...Hsiu-Chen Lin; June-Tai Wu; Bertrand Chin-Ming Tan; Cheng-Ting Chien In higher eukaryotes, the pre-replication complex (pre-RC) component Cdt1 is the major regulator in licensing control for DNA replication. The Cul4-DDB1-based ubiquitin ligase mediates Cdt1 ubiquitylation for subsequent...

...Nozomi Sugimoto; Kazumasa Yoshida; Yasutoshi Tatsumi; Takashi Yugawa; Mako Narisawa-Saito; Shou Waga; Tohru Kiyono; Masatoshi Fujita When human cells enter S-phase, overlapping differential inhibitory mechanisms downregulate the replication licensing factors ORC1, CDC6 and Cdt1. Such regulation...

...Yasutoshi Tatsumi; Nozomi Sugimoto; Takashi Yugawa; Mako Narisawa-Saito; Tohru Kiyono; Masatoshi Fujita The activity of human Cdt1 is negatively regulated by multiple mechanisms. This suggests that Cdt1 deregulation may have a deleterious effect. Indeed, it has been suggested that overexpression...

...Noah R. May; Marguerite Thomer; Katherine F. Murnen; Brian R. Calvi The regulation of a pre-replicative complex (pre-RC) at origins ensures that the genome is replicated only once per cell cycle. Cdt1 is an essential component of the pre-RC that is rapidly degraded at G1-S and also inhibited...

... . We have investigated regulation of the origin licensing factors Cdc6, Cdt1, Mcm2 and Geminin in human somatic and germ cells. Cdc6 and Cdt1 play an essential role in DNA replication initiation by loading the Mcm2-7 complex, which is required for unwinding the DNA helix, onto chromosomal origins...

..., is required for pre-replicative complex assembly by loading minichromosome maintenance proteins onto DNA. In fission yeast and Xenopus , this reaction known as the licensing of chromatin for DNA replication also requires the newly identified Cdt1 protein. We studied the role of hCdt1 protein...