... is a common feature of cancer cells, and thus a potentially important therapeutic target. Here, we show that cyclin-dependent kinase (CDK)-induced replication stress, resulting from Wee1 inactivation, is synthetic lethal with mutations disrupting dNTP homeostasis in fission yeast. Wee1 inactivation leads...

... report that Cdk-dependent TRF1 phosphorylation on T371 acts as a switch to create a pool of TRF1, referred to as (pT371)TRF1, which is recruited to ALT-associated PML bodies (APBs) in S and G2 phases independently of its binding to telomeric DNA. We find that phosphorylation of T371 is essential for APB...

... Cdk5 p25 p35 β-Amyloid Excitotoxicity Cdk Cyclins Neurodegeneration Alzheimer’s disease Synaptic plasticity Learning and memory Pain signalling Neuronal migration Axonal growth Synaptogenesis Cyclin-dependent kinases (Cdks) are Ser/Thr protein kinases that associate with specific...

... that is characterized by delayed chromosome decondensation and aberrant rearrangement of the meiotic spindle. The smg7 phenotype was mimicked by exposing meiocytes to the proteasome inhibitor MG115. Together, these data indicate that SMG7 counteracts cyclin-dependent kinase (CDK) activity at the end of meiosis...

...', or CDKs. And so Cdc2 became Cdk1... References Arion, D., Meijer, L., Brizuela, L. and Beach, D. ( 1988 ). Cdc2 is a component of the M-phase specific histone H1 kinase: evidence for identity with MPF. Cell 55 , 371 -378. Booher, R. and Beach, D. ( 1988 ). Involvement of cdc13+ in mitotic...

.... This has opened a new area of research into the dynamics of nuclear organization and the integration of nuclear functions. * Author for correspondence (e-mail: [email protected] ) © The Company of Biologists Limited 2002 2002 Nucleolus Cell cycle control CDK pol I transcription...

... ). Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen. Cell 37 , 801 -813. Lanker, S., Valdivieso, M. H. and Wittenberg, C. ( 1996 ). Rapid degradation of the G1 cyclin Cln2 induced by CDK-dependent phosphorylation. Science 271 , 1597 -1601. Lee, J...

...Mary E. Miller; Frederick R. Cross ABSTRACT Cyclin-dependent kinase (CDK) activity is essential for eukaryotic cell cycle events. Multiple cyclins activate CDKs in all eukaryotes, but it is unclear whether multiple cyclins are really required for cell cycle progression. It has been argued...

... chain reaction converts the poised, but inactive, MCM complex into an enzymatically active helicase. A growing list of proteins, including Mcm10 and Cdt1, are involved in the recruitment process. Two protein kinases, the Cdc7-Dbf4 kinase (DDK) and the cyclin-dependent kinase (CDK), trigger a chain...

... cell lines were compared. Cells expressing wild-type Akt were not susceptible to the growth-arresting effect of NGF, whereas parental PC12 cells and notably cells expressing mutant Akt were so affected. Accompanying this, the expressions of CDKs and p21 WAF1 were down- and up-regulated, respectively...

...Philipp Kaldis; Zachary W. Pitluk; I. Amy Bany; Deborah A. Enke; Marisa Wagner; Edward Winter; Mark J. Solomon ABSTRACT Eukaryotic cell cycles are controlled by the activities of cyclin-dependent kinases (cdks). The major cdk in budding yeast, Saccharomyces cerevisiae , is Cdc28p. Activation...

...’ to initiate the positive feedback loop at the entry into M phase. * Author for correspondence (e-mail: [email protected] ) 15 06 1998 30 07 1998 © 1998 by Company of Biologists 1998 cdc25 Cell cycle Phosphorylation cdk Phosphatase For microinjection...

... + in S. cerevisiae restores the G 1 arrest phenotype of cdc4 sic1 Δ double mutants. Overproduction of rum1 + in Saccharomyces cerevisiae causes a cell cycle block in G 1 with a phenotype similar to inactivation of all the Clb cyclins. Finally, we have mapped the cyclin interacting domain and Cdk...

... metazoan development. Now we can study how the crucial regulators of eukaryotic cell cycle progression, the various cyclin/cdk complexes (for a recent review see Nigg (1995) BioEssays 17, 471-480), are turned on or off during development. In Drosophila , this analysis is most advanced, in particular...

... or EGF+serum in dog thyroid epithelial cells suggests a major difference at mid to late G 1 stages between cAMP-dependent and cAMP-independent mitogenic pathways. * Author for correspondence 02 01 1996 23 04 1996 © 1996 by Company of Biologists 1996 CKI CDK Cell cycle...

.... Affinity-depletion of cyclin dependent kinases (Cdks) from these extracts blocks the initiation of DNA replication. We define ‘S-phase promoting factor’ (SPF) as the Cdk activity required for DNA replication in these Cdk-depleted extracts. Recombinant cyclins A and E, but not cyclin B, showed significant...

... replication or spindle assembly. In many organisms, transition points seem to involve regulation of the activity of cyclin-dependent kinases (cdks) not only through their interactions with various cyclins, but also by phosphorylation-dephosphorylation cycles acting on the kinase activity of the cdks...

...Steven I. Reed; Eric Bailly; Vjekoslav Dulic; Ludger Hengst; Dalia Resnitzky; Joyce Slingerland Cyclin-dependent kinases (Cdks) control the major cell cycle transitions in eukaryotic cells. On the basis of a variety of experiments where cyclin function either is impaired or enhanced, D-type cyclins...

...G. Maridor; P. Gallant; R. Golsteyn; E. A. Nigg ABSTRACT Cyclins control the activities of cyclin-dependent protein kinases (cdks) and hence play a key role in cell cycle regulation. While B-type cyclins associate with p34 cdc2 to trigger entry into mitosis, progression through S phase requires...

... a mechanism for coupling cell-cycle events to transcriptional initiation. * Corresponding author © The Company of Biologists Limited 1992 1992 cyclin transcription retinoblastoma CDK tumour suppressor Cyclins are an evolutionarily conserved group of regulatory molecules...