... is initiated during the cell cycle is not fully understood. By performing a proteomic analysis of transcription factors that bind RNA polymerase I during rDNA transcription initiation, we identified that the DNA replication initiator Cdc6 interacts with RNA polymerase I and its co-factors, and promotes rDNA...

... acquire the competence to replicate DNA one hour after the first meiotic division by accumulating an essential factor of the replicative machinery, Cdc6. Here, we show that the turnover of Cdc6 is precisely regulated in oocytes to avoid inhibition of Cdk1. At meiosis resumption, Cdc6 is expressed...

...Linda Clijsters; Rob Wolthuis ABSTRACT Cdc6 and Cdt1 initiate DNA replication licensing when cells exit mitosis. In cycling cells, Cdc6 is efficiently degraded from anaphase onwards as a result of APC/C–Cdh1 activity. When APC/C–Cdh1 is switched off again, at the end of G1 phase, Cdc6 could thus re...

...Kazumasa Yoshida; Nozomi Sugimoto; Satoko Iwahori; Takashi Yugawa; Mako Narisawa-Saito; Tohru Kiyono; Masatoshi Fujita CDC6, a replication licensing protein, is partially exported to the cytoplasm in human cells through phosphorylation by Cdk during S phase, but a significant proportion remains...

...Nozomi Sugimoto; Kazumasa Yoshida; Yasutoshi Tatsumi; Takashi Yugawa; Mako Narisawa-Saito; Shou Waga; Tohru Kiyono; Masatoshi Fujita When human cells enter S-phase, overlapping differential inhibitory mechanisms downregulate the replication licensing factors ORC1, CDC6 and Cdt1. Such regulation...

... . We have investigated regulation of the origin licensing factors Cdc6, Cdt1, Mcm2 and Geminin in human somatic and germ cells. Cdc6 and Cdt1 play an essential role in DNA replication initiation by loading the Mcm2-7 complex, which is required for unwinding the DNA helix, onto chromosomal origins...

... of pre-replicative complexes resulting in chromatin being ‘licensed’ for DNA replication in the subsequent S phase. We have analysed regulation of the pre-replicative complex proteins ORC, Cdc6, and MCM in cycling and non-proliferating quiescent, differentiated and replicative senescent human cells...

...Dawn Coverley; Cristina Pelizon; Sarah Trewick; Ronald A. Laskey ABSTRACT Cdc6 is essential for the initiation of DNA replication in all organisms in which it has been studied. In addition, recombinant Cdc6 can stimulate initiation in G 1 nuclei in vitro. We have analysed the behaviour...

... of Biochemistry, University of Dundee, Dundee DD1 5EH, Scotland, UK 29 03 1999 26 05 1999 © 1999 by Company of Biologists 1999 DNA replication Licensing factor ORC Cdc6 RLF In eukaryotes the initiation of replication forks occurs at multiple replication origins scattered...

...Corrella S. Detweiler; Joachim J. Li ABSTRACT CDC6 is essential for the initiation of DNA replication in the budding yeast Saccharomyces cerevisiae . Here we examine the timing of Cdc6p expression and function during the cell cycle. Cdc6p is expressed primarily between mitosis and Start...