... be inactivated to permit exit from mitosis, licensing of replication origins and cytokinesis. To study the role of Cdc2p in greater detail, we generated a cdc2 allele that is sensitive to an inhibitory ATP analogue. We show that the inhibitor-induced cell cycle arrest is reversible and examine the effect...

...In Sun Han; Tae Beom Seo; Kwan-Hoi Kim; Jin-Hwan Yoon; Sung-Jin Yoon; Uk Namgung Schwann cell migration facilitates peripheral nerve regeneration after injury. We have recently found increased activation of Cdc2 kinase in regenerating sciatic nerves. Here we show that Cdc2 phosphorylation...

... of the cyclin-dependent kinase (Cdk) Cdc2 that regulates entry into mitosis. Studies of the cdc37 temperature-sensitive mutants show a genetic interaction with some cdc2 alleles and overexpression of cdc2 rescues the lethality of some cdc37 alleles at the restrictive temperature, suggesting that Cdc2...

...Gilliane Maton; Thierry Lorca; Jean-Antoine Girault; René Ozon; Catherine Jessus The success of cell division relies on the activation of its master regulator Cdc2-cyclin B, and many other kinases controlling cellular organization, such as Aurora-A. Most of these kinase activities are regulated...

... be phosphorylated in vitro by cell cycle kinases proximal to a putative nuclear localization signal (NLS), which substantiated a predicted casein kinase II (CKII)-cdc2 kinase-NLS (CcN) motif at position 180-239 and suggested that mSTI1 might move between the cytoplasm and the nucleus under certain cell cycle...

...Ariane Abrieu; Marcel Dorée; Daniel Fisher ABSTRACT Throughout oocyte maturation, and subsequently during the first mitotic cell cycle, the MAP kinase cascade and cyclin-B–Cdc2 kinase are associated with the control of cell cycle progression. Many roles have been directly or indirectly attributed...

...Marie Frank-Vaillant; Olivier Haccard; Catherine Thibier; Ozon René; Yannick Arlot-Bonnemains; Claude Prigent; Catherine Jessus ABSTRACT Xenopus prophase oocytes reenter meiotic division in response to progesterone. The signaling pathway leading to Cdc2 activation depends on neosynthesized proteins...

...Anthi Karaïskou; Catherine Jessus; Thierry Brassac; René Ozon ABSTRACT The auto-catalytic activation of the cyclin-dependent kinase Cdc2 or MPF (M-phase promoting factor) is an irreversible process responsible for the entry into M phase. In Xenopus oocyte, a positive feed-back loop between Cdc2...

...Nicholas J. Wells; Nobumoto Watanabe; Tsuyoshi Tokusumi; Wei Jiang; Mark A. Verdecia; Tony Hunter ABSTRACT Activation of Cdc2, is the universal event controlling the onset of mitosis. In higher eukaryotes, Cdc2 activity is in part regulated by inhibitory phosphorylation of Thr14 and Tyr15...

... as they attempted to enter mitosis, which was accompanied by tyrosine dephosphorylation of Cdc2. Despite this, the caffeine-induced loss of viability was not blocked in a temperature-sensitive cdc2 mutant incubated at the restrictive temperature, although catastrophic mitosis was prevented under these conditions...

... and of Cdc2 and cyclin B (the catalytic and regulatory subunits of MPF) were followed by dissection of intact eggs following freezing and in cultured fragments separated by ligation. Cdc2 was found to be distributed evenly throughout the egg cytoplasm. Loss of phosphorylated (inactive) forms of Cdc2 coincided...

...Karim Labib; Sergio Moreno; Paul Nurse ABSTRACT The p34 cdc2 kinase is essential for progression past Start in the G 1 phase of the fission yeast cell cycle, and also acts in G 2 to promote mitotic entry. Whilst very little is known about the G 1 function of cdc2 , the rum1 gene has recently been...

...Weg Ongkeko; David J. P. Ferguson; Adrian L. Harris; Chris Norbury ABSTRACT A number of lines of evidence have suggested a possible involvement of the mitosis-promoting protein kinase Cdc2 in the process of apoptotic cell death, and one recent study concluded that premature activation of Cdc2...

...Anne-Marie Genevière-Garrigues; Abdelhamid Barakat; Marcel Dorée; Jean-Luc Moreau; André Picard ABSTRACT Feedback mechanisms preventing M phase occurrence before S phase completion are assumed to depend on inhi bition of cyclin B-cdc2 kinase activation by unreplicated DNA. In sea urchin...

... severely inhibits initiation of S phase. We have used p13 suc1 beads to remove both cdk2 and cdc2 proteins from egg extracts and developed a method to replace either protein alone to assess their capacity to initiate DNA replication. Re-addition of either cdk2 or cdc2 proteins to depleted extracts, through...

... the 55 kDa subunit. The reduced levels of the 55 kDa subunit correlate with the loss of protein phosphatase 2A-like, okadaic acid-sensitive phosphatase activity of brain extracts against caldesmon and histone H1 phospho-rylated by p34 cdc2 /cyclin B kinase, but not against phos-phorylase a . Thus...

...Jeannie Paris; Pascal Leplatois; Paul Nurse ABSTRACT In the fission yeast Schizosaccharomyces pombe , cdc2 function is required both in G 1 to enter the cell cycle and in G 2 to initiate mitosis. In higher eukaryotes, these functions appeared to be shared between several cdc2-like genes including...

... phase, we have also found that at the onset of mitosis RP-A is quantitatively phosphorylated and that phos-phorylation is directly mediated by cdc2 kinase. How-ever, at this time during the cell cycle, cdc2-dependent phosphorylation of RP-A is independant of DNA bind-ing. These observations further...

... by maturation promoting factor (MPF: p34 cdc2 /cyclin B complex), little is known about how p34 cdc2 kinase controls microtubule networks. We provide evidence of the direct association of the p34 cdc2 /cyclin B complex with microtubules in starfish oocytes. Anti-cyclin B staining of detergent-treated oocytes...

...Wilhelm Krek; John Marks; Nicole Schmitz; Erich A. Nigg; Viesturs Simanis ABSTRACT We have used the fission yeast Schizosaccharomyces pombe to analyse the effects of in vitro mutagenesis of the four known phosphorylation sites in the chicken p34 cdc2 protein, Thr14, Tyr15, Thr161 and Ser277, upon...