...Qian Zhang; Yue Zhang; Yaming Jiu ABSTRACT Zika virus (ZIKV) has gained notoriety in recent years because there are no targeted therapies or vaccines available so far. Caveolin-1 (Cav-1) in host cells plays crucial functions in the invasion of many viruses. However, its specific involvement in ZIKV...

... activation and cancer cell migration, but little is known about the upstream regulators of Rab5. We have previously shown that the scaffolding protein Caveolin-1 (CAV1) promotes Rac1 activation and migration of cancer cells. Here, we hypothesized that CAV1 stimulates Rab5 activation, leading to increased...

...Asier Echarri; Olivia Muriel; Dácil M. Pavón; Hind Azegrouz; Fernando Escolar; María C. Terrón; Fátima Sanchez-Cabo; Fernando Martínez; María C. Montoya; Oscar Llorca; Miguel A. del Pozo Summary The biology of caveolin-1 (Cav1)/caveolae is intimately linked to actin dynamics and adhesion receptors...

... the F-actin-dependent linear distribution of caveolin-1. High spatiotemporal resolution particle tracking of caveolin-1–GFP vesicles by total internal reflection fluorescence (TIRF) microscopy revealed that FLNa is required for the F-actin-dependent arrest of caveolin-1 vesicles in a confined area...

... in the dynamics and localization of caveolae during mitosis. During interphase, the equilibrium between the arrival and departure of caveolae from the cell surface maintains the steady-state distribution of caveolin-1 (Cav1) at the plasma membrane. During mitosis, increased dynamics coupled to an imbalance...

... in the barrier for large solutes. We observed that overexpression of occludin increased and occludin knockdown decreased sensitivity to cytokines as assessed by both these parameters. It is known that caveolin-1 interacts with occludin and is implicated in several models of cytokine-dependent barrier disruption...

... that Rac1 binds the adapter protein caveolin-1 (Cav1) and that Rac1 activity promotes Cav1 accumulation at Rac1-positive peripheral adhesions. Using Cav1-deficient mouse fibroblasts and depletion of Cav1 expression in human epithelial and endothelial cells mediated by small interfering RNA and short hairpin...

...-dependent activation of integrins remain important issues. The present study was undertaken to identify the molecular intermediates involved in the uPAR signaling pathway controlling α5β1-integrin activation and fibronectin polymerization. Disruption of lipid rafts with MβCD or depletion of caveolin-1...

...Parijat Sengupta; Finly Philip; Suzanne Scarlata Caveolae are membrane domains having caveolin-1 (Cav1) as their main structural component. Here, we determined whether Cav1 affects Ca 2+ signaling through the Gα q –phospholipase-Cβ (PLCβ) pathway using Fischer rat thyroid cells that lack Cav1...

... of caveolin-1 and caveolae during early embryonic development. During development, expression is apparent in a number of tissues including Kupffer's vesicle, tailbud, intersomite boundaries, heart, branchial arches, pronephric ducts and periderm. Particularly strong expression is observed in the sensory...

... invaginations of the cell membrane; their major structural protein, caveolin-1, has been shown to regulate signaling molecules localized in these micro-domains. The interaction of caveolin-1 with several of these proteins is mediated by the binding of its scaffolding domain to a region containing hydrophobic...

...Vicente A. Torres; Julio C. Tapia; Diego A. Rodríguez; Mario Párraga; Pamela Lisboa; Margarita Montoya; Lisette Leyton; Andrew F. G. Quest Caveolin-1 is suggested to act as a tumor suppressor. We tested the hypothesis that caveolin-1 does so by repression of survivin, an Inhibitor of apoptosis...

... 116 cell line with a gene encoding antisense caveolin-1 (AS-cav-1) to examine the effects of caveolin-1, the main structural protein of caveolae, on the expression and localization of cathepsin B and pro-uPA, and their cell-surface receptors p11 and uPA receptor (uPAR), respectively. AS-cav-1 HCT 116...

...Anja Nohe; Eleonora Keating; T. Michael Underhill; Petra Knaus; Nils O. Petersen Caveolae are small invaginations of the cell membrane that are thought to play a role in important physiological functions such as cell surface signaling, endocytosis and intracellular cholesterol transport. Caveolin-1...

... proteins simultaneously. MAL, BENE and MAL2 co-fractionated with caveolin-1 in the raft fraction of PC-3 cells, and immunofluorescence analysis showed colocalization of these proteins with caveolin-1 in a multivesicular intracellular compartment. Markers of the Golgi apparatus, early and recycling...

.... The high-resolution immunogold technique revealed labelling mainly over microvilli of the enterocyte. SR-BI was not associated with caveolin-1 and was not detectable in caveolae. In order to define the role of SR-BI in intestinal cholesterol absorption, Caco-2 cells were transfected with a constitutive...

...Wei-Ping Li; Pingsheng Liu; Brian K. Pilcher; Richard G. W. Anderson ABSTRACT In commonly used tissue culture cells, caveolin-1 is embedded in caveolae membranes. It appears to reach this location after being cotranslationally inserted into ER membranes, processed in the Golgi and shipped...

...Toyoshi Fujimoto; Hiroshi Kogo; Ryuji Nomura; Tomoko Une ABSTRACT The relationship between caveolin-1 isoforms (α and β) and caveolar ultrastructure was studied. By immunofluorescence microscopy of human fibroblasts, caveolae were observed as dots positive for caveolin-1, but many dots labeled...