... stopped by adding SDS sample buffer and boiling, then analyzed by SDS PAGE followed by autoradiography, or Coomassie Blue staining to stain for total histone H1. Cells expressing 3HA tagged cyclin B were untreated, treated with 1 µM calyculin A or 0.2 µM staurosporine as indicated, then lysed under...

... Kalirin function throughout development. * Author for correspondence (e-mail: [email protected]) 16 6 2008 © The Company of Biologists Limited 2008 2008 RhoGEF PC12 cells Spectrin-like repeats PP-1 Phosphorylation Roscovitine Calyculin A In mammals, flies and worms...

... to inhibit protein phos-phatases 1 and/or 2A, because okadaic acid tetraacetate, which is not a phosphatase inhibitor, has no effect. Dephos-phorylation of H1 does not occur if okadaic acid-treated cells are simultaneously treated with 20 nM calyculin A, or if the okadaic acid concentration is 5.0 μM...

... kinase C inhibitor Ro 31-8220 (10 6 to 10 5 M). Ro 31-8220 (10 5 M) alone increased CD18 expression of initially resting cells. Furthermore, the phosphatase inhibitor okadaic acid (5 M) and calyculin A (0.04 μM) suppressed CD18 expression in chemotactic peptide-treated cells, but there was no detectable...