... depolymerization. Here, we show that UNC-87, a calponin-related Caenorhabditis elegans protein with seven calponin-like repeats, competes with ADF/cofilin for binding to actin filaments and inhibits ADF/cofilin-dependent filament severing and depolymerization in vitro. Mutations in the unc-87 gene suppress...

...Samudra S. Gangopadhyay; Norio Takizawa; Cynthia Gallant; Amy L. Barber; Hyun-Dong Je; Tara C. Smith; Elizabeth J. Luna; Kathleen G. Morgan The mechanisms by which protein kinase C (PKC) and extracellular-signal-regulated kinases (ERK1/2) govern smooth-muscle contractility remain unclear. Calponin...

...Gerald Burgstaller; Wolfgang J. Kranewitter; Mario Gimona The three genetic isoforms of calponin (CaP), h 1, h 2 and acidic, are distinguished mostly by their individual C-terminal tail sequences. Deletion of these sequences beyond the last homologous residue Cys273 increases actin filament...

...Claudia Danninger; Mario Gimona ABSTRACT The calponin family of F-actin-, tropomyosin- and calmodulin-binding proteins currently comprises three genetic variants. Their functional roles implicated from in vitro studies include the regulation of actomyosin interactions in smooth muscle cells (h 1...

...Mario Gimona; Renu Mital ABSTRACT Calponins have been implicated in the regulation of actomyosin interactions in smooth muscle cells, cytoskeletal organisation in nonmuscle cells, and the control of neurite outgrowth. Domains homologous to the amino-terminal region of calponin have been identified...

...Alison J. North; Mario Gimona; Robert A. Cross; J. Victor Small ABSTRACT Calponin and caldesmon are two thin filament-binding proteins found in smooth muscle that have both been attributed a role in modulating the interaction of actin and myosin. Using high-resolution dual-label immunocyto...