... in the Ca 2+ sensor calmodulin (CaM) have been associated with CPVT susceptibility, suggesting that CaM dysfunction is a key driver of the disease. However, the detailed molecular mechanism remains unclear. Focusing on the interaction with the cardiac ryanodine receptor (RyR2), we determined the effect...

...+ ] c and reactivated these cells. The ionocyte reactivation was attenuated by chelating intracellular Ca 2+ and inhibiting calmodulin (CaM), suggesting involvement of a Ca 2+ and CaM-dependent mechanism. Long-term imaging studies showed that after an initial decrease, [Ca 2+ ] c gradually returned...

... a functionally deficient version of DHHC21, we show that DHHC21 is a Ca 2+ /calmodulin-dependent enzyme critical for activation of naïve CD4 + T cells in response to T cell receptor stimulation. We find that disruption of the Ca 2+ /calmodulin-binding domain of DHHC21 does not affect thymic T cell development...

... oxide (NO) generation is dependent on Ca 2+ and calmodulin (CaM). Here, we used Arabidopsis thaliana transgenic seedlings expressing cyan fluorescent protein (CFP) containing a type 1 peroxisomal-targeting signal motif (PTS1; CFP–PTS1), which enables peroxisomes to be visualized in vivo , and also used...

...Araitz Alberdi; Carolina Gomis-Perez; Ganeko Bernardo-Seisdedos; Alessandro Alaimo; Covadonga Malo; Juncal Aldaregia; Carlos Lopez-Robles; Pilar Areso; Elisabeth Butz; Christian Wahl-Schott; Alvaro Villarroel ABSTRACT We show that the combination of an intracellular bi-partite calmodulin (CaM...

...Carolina Gomis-Perez; Alessandro Alaimo; Juncal Fernandez-Orth; Araitz Alberdi; Paloma Aivar-Mateo; Ganeko Bernardo-Seisdedos; Covadonga Malo; Pilar Areso; Antonio Felipe; Alvaro Villarroel ABSTRACT Calmodulin (CaM) binding to the AB module is crucial for multiple mechanisms governing the function...

...Xiaojun Huang; Ying Liu; Ruiwu Wang; Xiaowei Zhong; Yingjie Liu; Andrea Koop; S. R. Wayne Chen; Terence Wagenknecht; Zheng Liu Summary Calmodulin (CaM), a 16 kDa ubiquitous calcium-sensing protein, is known to bind tightly to the calcium release channel/ryanodine receptor (RyR), and modulate RyR...

...Alessandro Alaimo; Araitz Alberdi; Carolina Gomis-Perez; Juncal Fernández-Orth; Juan Camilo Gómez-Posada; Pilar Areso; Alvaro Villarroel Summary Among the multiple roles assigned to calmodulin (CaM), controlling the surface expression of Kv7.2 channels by binding to two discontinuous sites...

... currents occurs in the continuous presence of elevated Ca 2+ concentrations, possibly by calmodulin-dependent kinase. The present results demonstrate that anoctamins are a family of Ca 2+ -activated Cl − channels, which also induce permeability for cations. They may operate as Cl − channels located...

... of attracting monocytes. Additionally, we showed that Nef-induced CCL2/MCP-1 expression depends on the myristoylation moiety of Nef and requires functional calmodulin. In summary, we suggest that Nef-induced CCL2/MCP-1 expression in astrocytes contributes to infiltration of monocytes into the brain, and thereby...

...Erin E. Dymek; Daniel Goduti; Tal Kramer; Elizabeth F. Smith Kinesin-like calmodulin-binding protein, KCBP, is a novel member of the C-kinesin superfamily first discovered in flowering plants. This minus-end-directed kinesin exhibits Ca 2+ -calmodulin-sensitive motor activity in vitro and has been...

... specifically precipitated five polypeptides in addition to PF6. Using mass spectrometry, we determined the amino acid identities of these five polypeptides. Most notably, the PF6-containing complex includes calmodulin. Using antibodies generated against each precipitated polypeptide, we confirmed...

...Cynthia Gallant; Jae Young You; Yasuharu Sasaki; Zenon Grabarek; Kathleen G. Morgan Calmodulin (CaM) is a ubiquitous transducer of intracellular Ca 2+ signals and plays a key role in the regulation of the function of all cells. The interaction of CaM with a specific target is determined not only...

... in the maintenance of motility; nevertheless, how Ca 2+ modulates this process has not yet been completely characterized. Ca 2+ can bind to calmodulin and this complex regulates the activity of multiple enzymes, including Ca 2+ /calmodulin-dependent protein kinases (CaM kinases). Results from this study confirmed...

... and calmodulin, but the significance of this interaction on Kir/Gem function is poorly understood. Here, we present a comprehensive analysis of the binding of 14-3-3 and calmodulin to Kir/Gem. We show that 14-3-3, in conjunction with calmodulin, regulates the subcellular distribution of Kir/Gem between...

...Hai-Tao Zeng; Daulat R. P. Tulsiani Sperm capacitation in vitro is thought to be correlated with the increased protein tyrosine phosphorylation of a subset of sperm components. Our group recently used a pharmacological approach to demonstrate that calmodulin (CaM),a 17 kDa calcium sensor protein...

... was conducted to investigate the contributions of the calcium-binding protein calmodulin and of calcium—calmodulin-dependent protein kinase II to cytosolic phospholipase A 2 translocation to the nuclear envelope elicited by norepinephrine in rabbit aortic smooth-muscle cells. Norepinephrine caused cytosolic...

... a stable complex with EEA1. Early endosome fusion requires the release of intravesicular calcium, and calmodulin plays a vital role in the fusion pathway, as judged by sensitivity to antagonists. We demonstrate that both EEA1 and syntaxin 13 interact with calmodulin. In the case of EEA1, binding...

... that calmodulin may be associated with the mechanism underlying flagellar quiescence induced by 10 −4 M Ca 2+ . ‡ Author for correspondence (e-mail: [email protected] ) * Present address: Department of Physiology, University of Massachusetts Medical Center, Worcester, MA 01655, USA 10...

... in G 1 for its cell cycle function. Both the actin and microtubule defects of arc35-1 can be suppressed by overexpression of calmodulin. Analysis of a collection of ts cmd1 mutants for their ability to suppress the actin and/or microtubule defect revealed that the two defects observed in arc35-1...