...Sara K. Tucker; Douglas M. McLaurin; Michael D. Hebert ABSTRACT Cajal bodies (CBs) are membraneless organelles whose mechanism of formation is still not fully understood. Many proteins contribute to the formation of CBs, including Nopp140 (NOLC1), WRAP53 and coilin. Coilin is modified on multiple...

...Davide A. Basello; A. Gregory Matera; David Staněk ABSTRACT Coilin is a conserved protein essential for integrity of nuclear membrane-less inclusions called Cajal bodies. Here, we report an amino acid substitution (p.K496E) found in a widely-used human EGFP–coilin construct that has a dominant...

.... 28 04 2014 8 07 2014 © 2014. Published by The Company of Biologists Ltd 2014 Cajal bodies Gems Nuclear structure SMN U1 snRNP U1-70K The U1 small nuclear ribonucleoprotein particle (U1 snRNP) is an essential component of the spliceosome, a large macromolecular complex...

... that SMN relocated to damaged interphase centromeres, together with the Cajal-body-associated proteins coilin and fibrillarin, during the so-called interphase centromere damage response (iCDR). Here we reveal that SMN is a chromatin-binding protein that specifically interacts with methylated histone H3K79...

... for the assembly of spliceosomal small nuclear ribonucleoproteins (snRNPs), their nuclear import and their localization to subnuclear domain Cajal bodies (CBs). The concentration of the SMN complex and snRNPs in CBs is reduced upon SMN deficiency in SMA cells. Subcellular localization of the SMN complex...

... ribonucleoproteins (snRNPs). The protein is detected in the cytoplasm, nucleoplasm and enriched with snRNPs in nuclear Cajal bodies. It is structurally divided into at least an amino-terminal region rich in basic amino acid residues, a central Tudor domain, a self-association tyrosine-glycine-box and an exon7...

... pore complexes and extend throughout the nucleus. Within the nucleus, these `pore-linked filaments' (PLFs) were embedded into spherical structures 100 nm to ∼5 μm in diameter. A subset of spheres was identified as Cajal bodies by immuno-gold labeling; the rest were inferred to be nucleoli...

.... Cajal bodies labelled with either FP-SMN or FP-coilin show similar behaviour and frequency of movements. However, fluorescence recovery after photobleaching (FRAP)studies show that SMN returns ∼50-fold more slowly to Cajal bodies than does coilin. Time-lapse studies on cells progressing from prophase...

... allowed us to characterize the nuclear pathway that leads to snRNP accumulation in nuclear speckles and has identified a limiting nucleolar step in the pathway that can be saturated by overexpression of Sm proteins. After nuclear import, newly assembled snRNPs accumulate first in a subset of Cajal bodies...