... , A. H. , Abuwarda , H. , Tombola , F. , Flanagan , L. A. , Dunn , A. R. et al. ( 2019 ). Myosin-II mediated traction forces evoke localized Piezo1-dependent Ca2+ flickers . Commun. Biol. 2 , 298 . 10.1038/s42003-019-0514-3 Ezratty , E. J. , Partridge , M...

.../j.jbior.2013.09.001 Kilpatrick , B. S. , Eden , E. R. , Schapira , A. H. , Futter , C. E. and Patel , S. ( 2013 ). Direct mobilisation of lysosomal Ca2+ triggers complex Ca2+ signals . J. Cell Sci. 126 , 60 - 66 . 10.1242/jcs.118836 Kim , G. H. , Dayam...

.... The peer review history is available online at https://journals.biologists.com/jcs/lookup/doi/10.1242/jcs.259823.reviewer-comments.pdf . References Ashrafi , G. , de Juan-Sanz , J. , Farrell , R. J. and Ryan , T. A. ( 2020 ). Molecular tuning of the axonal mitochondrial Ca2...

.... ( 2006 ). The calcineurin pathway links hyperpolarization (Kir2.1)-induced Ca2+ signals to human myoblast differentiation and fusion . Development 133 , 3107 - 3114 . 10.1242/dev.02479 Lam , D. and Schlichter , L. C. ( 2015 ). Expression and contributions of the Kir2.1 inward...

... , S. ( 1999 ). Ca2+-induced increased lipid packing and domain formation in submitochondrial particles. A possible early step in the mechanism of Ca2+-stimulated generation of reactive oxygen species by the respiratory chain . Biochemistry 38 , 13279 - 13287 . 10.1021/bi9828674...

... aggregated and dispersed insulin-producing cells . Diabetologia 56 , 1557 - 1568 . 10.1007/s00125-013-2903-3 Chung , W. Y. , Jha , A. , Ahuja , M. and Muallem , S. ( 2017 ). Ca2+ influx at the ER/PM junctions . Cell Calcium 63 , 29 - 32 . 10.1016/j.ceca.2017.02.009...

.... , Katris , N. J. , Hapuarachchi , S. V. , Coffey , M. J. , Lehane , A. M. et al. ( 2018 ) Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii . PLoS Biol. 16 , e2005642 . 10.1371/journal.pbio.2005642 van Dooren , G. G. , Stimmler , L. M...

.... , Miao , Y. L. , Armstrong , D. L. and Williams , C. J. E. ( 2015 ). CaV3.2 T-type channels mediate Ca2+ entry during oocyte maturation and following fertilization . J. Cell Sci. 93 , 13004 . 10.1242/jcs.180026 Bernhardt , M. L. , Stein , P. , Carvacho , I...

.../j.cell.2007.11.028 Danthuluri , N. R. , Kim , D. and Brock , T. A. ( 1990 ). Intracellular alkalinization leads to Ca2+ mobilization from agonist-sensitive pools in bovine aortic endothelial cells . J. Biol. Chem. 265 , 19071 - 19076 . 10.1016/S0021-9258(17)30626-9...

... depict the mean±s.e.m. percentage change in the integrated Ca 2+ responses of the moderate and high mYFP-expressing cells relative to non-expressing cells within the same sample ( n ≥3 for all conditions). Fig. 7. Vav2 acts through Cdc42 to inhibit TCR-induced Ca2+ responses. (A) Representative Ca2...

... blocked ANOVA. Fig. 5. Inhibition of MRP4 increases Ca2+ levels. (A) Intracellular levels of Ca2+ in spermatozoa incubated in CAP medium in the presence or absence of MK571 (50 µM) or A23187 (10 µM). After treatment, spermatozoa were loaded with Fluo3-AM and PI and the fluorescence of each probe...

... of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. (B) Quantification of the subplasma membrane density change following 15 min exposure to 20 mM glucose (n=36 cells; P>0.1, paired two-tailed Student's t-test). (C) TIRF microscopy recording of the cytosolic Ca2...

...) stimulated with histamine (100 µM, arrow). Thin dashed lines show the ±95% confidence limits for the mean fluorescence ratios. The lower panel shows the cumulative plot of histamine-evoked WPB fusion times scaled to the mean fraction of WPBs that underwent exocytosis. Fig. 4. Ca2+-independent SYT5 mutant...

...+ in the cytosol or cell wall. Fig. 1. In vivo detection of Ca2+, peroxisomes and chloroplasts in Arabidopsis guard and root cells. Representative CLSM images of (A–E) guard cells and (F–I) root cells of 5-day-old transgenic Arabidopsis seedlings expressing CFP–PTS1. Ca2+ is shown in red, peroxisomes in green...

... Ca2+, CaM and the involvement of several peroxins (PEXs), including PEX12, which is located in the membrane, and both the PTS receptors (PEX5 and PEX7), located in the cytosol. Following the import of the NOS protein into the matrix, PTS2 is cleaved by a glyoxysomal-processing protease (GPP). Ca2...

.... Scale bars: 10 μm. Fig. 1. Dynamic imaging of the interaction between STIM1–CC1 and SOAR domains in situ. (A) Schematics of STIM1 structure with segment boundaries relevant to the present study. Numbering corresponds to the human STIM1 protein. EF, Ca2+ -binding EF-hand motif; SAM, sterile alfa motif...

... (unpaired t -test). Fig. 3. TRPC1 silencing attenuates non-stimulated Ca2+ influx and augments store-operated Ca2+ entry after hypoxia in MDA-MB-468 breast cancer cells. (A) Mean [Ca2+]CYT levels during assessment of non-stimulated Ca2+ influx (DMSO control) and store-operated Ca2+ entry (SOCE; 10 µM CPA...