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... excitatory pathway involved in learning and memory. References Ashrafi , G. , de Juan-Sanz , J. , Farrell , R. J. and Ryan , T. A. ( 2020 ). Molecular tuning of the axonal mitochondrial Ca2+ uniporter ensures metabolic flexibility of neurotransmission . Neuron 105 , 678 - 687...

.../jbc.M313932200 Konig , S. , Béguet , A. , Bader , C. R. and Bernheim , L. ( 2006 ). The calcineurin pathway links hyperpolarization (Kir2.1)-induced Ca2+ signals to human myoblast differentiation and fusion . Development 133 , 3107 - 3114 . 10.1242/dev.02479 Lam , D...

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.... et al. ( 2018 ) Protein kinase A negatively regulates Ca2+ signalling in Toxoplasma gondii . PLoS Biol. 16 , e2005642 . 10.1371/journal.pbio.2005642 van Dooren , G. G. , Stimmler , L. M. and McFadden , G. I. ( 2006 ). Metabolic maps and functions of the Plasmodium...

...-94-007-0265-3_9 Bernhardt , M. L. , Zhang , Y. , Erxleben , C. F. , Padilla-Banks , E. , McDonough , C. E. , Miao , Y. L. , Armstrong , D. L. and Williams , C. J. E. ( 2015 ). CaV3.2 T-type channels mediate Ca2+ entry during oocyte maturation and following...

... ). Calcium signaling . Cell 131 , 1047 - 1058 . 10.1016/j.cell.2007.11.028 Danthuluri , N. R. , Kim , D. and Brock , T. A. ( 1990 ). Intracellular alkalinization leads to Ca2+ mobilization from agonist-sensitive pools in bovine aortic endothelial cells . J. Biol. Chem. 265...

.... Kymographs span the full 5 min imaging period. Fig. 1. Vav2 inhibits Ca2+ influx in Jurkat T cells. (A) The domain structures and predicted domain boundaries for the Vav proteins are depicted. Conserved regulatory tyrosine phosphorylation sites (yellow circles) and the GTPase binding site (grey circle...

... blocked ANOVA. Fig. 5. Inhibition of MRP4 increases Ca2+ levels. (A) Intracellular levels of Ca2+ in spermatozoa incubated in CAP medium in the presence or absence of MK571 (50 µM) or A23187 (10 µM). After treatment, spermatozoa were loaded with Fluo3-AM and PI and the fluorescence of each probe...

...-test). (E) TIRF microscopy recordings of subplasma membrane [Ca2+] (5HT6-G-GECO, black) and F-actin (mCherry-CHUtr, magenta) fluorescence during depolarization with 30 mM KCl (means±s.e.m. for 13 cells from four separate experiments). (F) TIRF microscopy image (left) and recordings (right...

...) stimulated with histamine (100 µM, arrow). Thin dashed lines show the ±95% confidence limits for the mean fluorescence ratios. The lower panel shows the cumulative plot of histamine-evoked WPB fusion times scaled to the mean fraction of WPBs that underwent exocytosis. Fig. 4. Ca2+-independent SYT5 mutant...

... several NADPH-recycling enzymes, including G6PDH, 6PGDH and NADP-ICDH. OPDA, 12-oxo-phytodienoic acid. Dashed lines with arrow indicate the signaling processes. Fig. 3. Model of the metabolic integration of NADPH, NO and Ca2+ in plant peroxisomes. The NADPH pool is necessary for different processes...

.... In vivo detection of Ca2+, peroxisomes and chloroplasts in Arabidopsis guard and root cells. Representative CLSM images of (A–E) guard cells and (F–I) root cells of 5-day-old transgenic Arabidopsis seedlings expressing CFP–PTS1. Ca2+ is shown in red, peroxisomes in green and chloroplasts in purple. (A,F...

... corresponds to the human STIM1 protein. EF, Ca2+ -binding EF-hand motif; SAM, sterile alfa motif; TM, transmembrane segment; CC1, CC2 and CC3, coiled-coil domains; SOAR, minimal Orai1 activation region; S/P, proline-serine-threonine-rich segment; K, polybasic domain. (B) Confocal images showing...

...). Fig. 3. TRPC1 silencing attenuates non-stimulated Ca2+ influx and augments store-operated Ca2+ entry after hypoxia in MDA-MB-468 breast cancer cells. (A) Mean [Ca2+]CYT levels during assessment of non-stimulated Ca2+ influx (DMSO control) and store-operated Ca2+ entry (SOCE; 10 µM CPA) with or without...

... cells. These observations were measured in 25 cells (results for compacted collagen are mean±s.e.m.). Experiments in A–C, and F–K were performed in FliI WT cells. Fig. 1. Role of TRP Ca2+ channels in collagen contraction. (A) Representative confocal image of a cell transfected with a plasma membrane...

.... The TRP mucolipin agonist ML-SA1 evokes global Ca2+ signals. (A–D) Effect of ML-SA1 on cytosolic Ca2+ levels. Cytosolic Ca2+ levels of individual Fura-2-loaded HeLa cells (A) or fibroblasts (B) from an exemplar population stimulated with increasing concentrations of ML-SA1. (C,D) Summary data quantifying...