...Tomohiro Yorimitsu; Ken Sato ABSTRACT COPII proteins assemble at ER exit sites (ERES) to form transport carriers. The initiation of COPII assembly in the yeast Saccharomyces cerevisiae is triggered by the ER membrane protein Sec12. Sec16, which plays a critical role in COPII organization, localizes...

.... Summary: TANGO1 is required to maintain the fundamental organization of the early secretory pathway in mammalian cells to support secretion of a diverse range of newly synthesized cargo proteins. Secretory pathway COPII Collagen Golgi TANGO1 ERGIC University of Bristol http...

... RER1, which cycles between cis-Golgi and ER, returning ER-resident proteins and unassembled subunits of multimeric complexes back to the ER. RER1 ER-Golgi Golgi-ER COPII COPI Transmembrane domain Vlaams Instituut voor Biotechnologie 10.13039/501100004727 C0401 WIAN KU...

...Laura M. Westrate; Melissa J. Hoyer; Michael J. Nash; Gia K. Voeltz ABSTRACT Secretory cargo is recognized, concentrated and trafficked from endoplasmic reticulum (ER) exit sites (ERES) to the Golgi. Cargo export from the ER begins when a series of highly conserved COPII coat proteins accumulate...

... of the COPII machinery. This article has an associated First Person interview with the first author of the paper . * Author for correspondence ( [email protected] ) Competing interests The authors declare no competing or financial interests. 15 3 2017 17 8 2017 © 2018. Published...

...Hirohiko Iwasaki; Tomohiro Yorimitsu; Ken Sato ABSTRACT The COPII coat and the small GTPase Sar1 mediate protein export from the endoplasmic reticulum (ER) via specialized domains known as the ER exit sites. The peripheral ER protein Sec16 has been proposed to organize ER exit sites. However...

... (the SAR1A isoform) small GTPase. Compared to palmitoylation-deficient Wnt molecules, binding of mature Wnt to Wls increases Wls–SEC12 interaction and promotes association of Wls with SAR1, the key activator of the COPII machinery. Incorporation of Wls into this exporting ER compartment is affected by Wnt...

... protein required for such membrane remodeling. We show that ER-vesicle protein of 14 kD (Erv14), a cargo receptor of coat protein complex II (COPII), interacts with BMV 1a. Moreover, the perinuclear ER localization of BMV 1a is disrupted in cells lacking ERV14 or expressing dysfunctional COPII coat...

...David Klinkenberg; Kimberly R. Long; Kuntala Shome; Simon C. Watkins; Meir Aridor ABSTRACT The inner and outer layers of COPII mediate cargo sorting and vesicle biogenesis. Sec16A and p125A (officially known as SEC23IP) proteins interact with both layers to control coat activity, yet the steps...

...-to-Golgi transport is crucial for maintaining the different membrane lipid composition and identities of organelles. Despite their importance, mechanisms regulating transport remain elusive. Here we report that in yeast coat protein complex II (COPII) vesicle-mediated transport of ceramide from the ER...

... residues in the N-terminus of the protein. Here, we describe the effects of Chs2p phosphorylation on the interaction with coat protein complex II (COPII). Identification of a cdc5 ts mutant, which fails to transport Chs2p–3xGFP to the bud-neck and instead accumulates the protein in intracellular puncta...

... expanded ER sheets and the plasma membrane. * These authors contributed equally to this work ‡ Author for correspondence ( [email protected] ) 19 2 2012 © 2012. Published by The Company of Biologists Ltd 2012 ERES COPII ER membrane curvature The secretory pathway...

...Anna K. Townley; Katy Schmidt; Lorna Hodgson; David J. Stephens Epithelial morphogenesis is directed by interactions with the underlying extracellular matrix. Secretion of collagen and other matrix components requires efficient coat complex II (COPII) vesicle formation at the endoplasmic reticulum...

...Helen Hughes; David J. Stephens Mitotic inhibition of COPII-dependent export of proteins from the endoplasmic reticulum results in disassembly of the Golgi complex. This ensures ordered inheritance of organelles by the two daughter cells. Reassembly of the Golgi is intimately linked to the re...

...Carine Bonnon; Markus W. Wendeler; Jean-Pierre Paccaud; Hans-Peter Hauri Selective export of transmembrane proteins from the endoplasmic reticulum (ER) relies on recognition of cytosolic-domain-localized transport signals by the Sec24 subunit of the COPII vesicle coat. Human cells express four...

...Helen Hughes; Annika Budnik; Katy Schmidt; Krysten J. Palmer; Judith Mantell; Chris Noakes; Andrew Johnson; Deborah A. Carter; Paul Verkade; Peter Watson; David J. Stephens The COPII complex mediates the selective incorporation of secretory cargo and relevant machinery into budding vesicles...

...., 1999 ; Forster et al., 2006 ; Guo and Linstedt, 2006 ; Heinzer et al., 2008 ). The inability of nocodazole to induce additional proliferation of ER exit sites in syntaxin-18-depleted cells may reflect a shortage of cycling components required for COPII assembly, which may be due to the inhibition...

...Anna K. Townley; Yi Feng; Katy Schmidt; Deborah A. Carter; Robert Porter; Paul Verkade; David J. Stephens The COPII coat assembles on endoplasmic reticulum membranes to coordinate the collection of secretory cargo with the formation of transport vesicles. During COPII assembly, Sar1 deforms...

... sorting to ERESs to be a slow and diffusion-unlimited process. Our findings exclude COPI, and identify the COPII protein complex to be directly involved in the secretory cargo sorting and redistribution functions of ERESs. * Author for correspondence (e-mail: [email protected] ) 11 12...

...Leah M. Welsh; Amy Hin Yan Tong; Charles Boone; Ole N. Jensen; Stefan Otte Erv41p and Erv46p are integral membrane proteins conserved across species. They were originally identified as abundant constituents of COPII-coated vesicles, and form a complex which cycles between the endoplasmic reticulum...