...Lawrence G. Welch; Nadine Muschalik; Sean Munro ABSTRACT Golgi-resident enzymes remain in place while their substrates flow through from the endoplasmic reticulum to elsewhere in the cell. COPI-coated vesicles bud from the Golgi to recycle Golgi residents to earlier cisternae. Different enzymes...

... RER1, which cycles between cis-Golgi and ER, returning ER-resident proteins and unassembled subunits of multimeric complexes back to the ER. RER1 ER-Golgi Golgi-ER COPII COPI Transmembrane domain Vlaams Instituut voor Biotechnologie 10.13039/501100004727 C0401 WIAN KU...

... blocking for 1 h in 3% bovine serum albumin (BSA) 1× PBS, the cells were probed with the chicken anti-COPI (HEN 1–347093; 1:250) and rat anti-Sec (RAT 1–347091; 1:250) antisera. Secondary Alexa Fluor 488-conjugated goat anti-chicken IgG (H-L), Alexa Fluor 488-conjugated chicken anti-rat IgG and Alexa Fluor...

...Eric C. Arakel; Blanche Schwappach ABSTRACT The coat protein complex I (COPI) allows the precise sorting of lipids and proteins between Golgi cisternae and retrieval from the Golgi to the ER. This essential role maintains the identity of the early secretory pathway and impinges on key cellular...

... maturation is fulfilled by the retrograde transport of Golgi-resident proteins from later to earlier cisternae, and candidate carriers for this retrograde transport are coat protein complex I (COPI)-coated vesicles. We examined the COPI function in cisternal maturation directly by 4D observation...

... at the extreme C-terminus of the channels. The current model proposes that phosphorylation-dependent binding of 14-3-3 sterically masks a COPI-binding motif. However, the direct effects of phosphorylation on COPI binding and on the binding parameters of 14-3-3 isoforms are still unknown. We find...

...Eric C. Arakel; Sören Brandenburg; Keita Uchida; Haixia Zhang; Yu-Wen Lin; Tobias Kohl; Bianca Schrul; Matthew S. Sulkin; Igor R. Efimov; Colin G. Nichols; Stephan E. Lehnart; Blanche Schwappach ABSTRACT The copy number of membrane proteins at the cell surface is tightly regulated. Many ion...

...Daishi Kitazawa; Masamitsu Yamaguchi; Hajime Mori; Yoshihiro H. Inoue Summary The coatomer protein complex, COPI, mediates retrograde vesicle transport from the Golgi apparatus to the ER. Here, we investigated the meiotic phenotype of Drosophila melanogaster spermatocytes expressing dsRNA of 52...

...Kristina Armbruster; Stefan Luschnig Protein trafficking through the secretory pathway plays a key role in epithelial organ development and function. The expansion of tracheal tubes in Drosophila depends on trafficking of coatomer protein complex I (COPI)-coated vesicles between the Golgi complex...

..., the integrity of the Golgi complex is impaired. As a result, both vesicle transport of cargo proteins and directed apical membrane delivery are severely disrupted. Dysfunction of the Arf1–COPI machinery caused by a loss of Garz leads to perturbations in establishing a polarized epithelial architecture...

...-gif.fr © The Company of Biologists Limited 2009 2009 Golgi Secretory pathway Intra-Golgi trafficking Membrane curvature ALPS motif Tether Golgin Sphingolipid Glycerophospholipid ADP ribosylation factor G protein COPI Coatomer Glycosylation Because of its structural...

... concentration within ERESs continued in cells overexpressing the GTP-locked ARF1Q71L or in the presence of brefeldin A. In the absence of COPI and microtubules, ERESs transformed from tubulovesicular to spherical membranes that actively accumulated secretory cargo and excluded ER-membrane markers. We found...

...Tomasz Szul; Robert Grabski; Susan Lyons; Yuichi Morohashi; Svetlana Shestopal; Martin Lowe; Elizabeth Sztul COPI recruitment to membranes appears to be essential for the biogenesis of the Golgi and for secretory trafficking. Preventing COPI recruitment by expressing inactive forms of the ADP...

... 18 4 2006 © The Company of Biologists Limited 2006 2006 ER-exit sites ER-to-Golgi transport ERGIC-53 Protein sorting COPI COPII Tethering Fusion The ER-Golgi intermediate compartment (ERGIC) is a complex membrane system between the rough endoplasmic reticulum (ER...

... the mechanism of its localization in the Golgi. At the nonpermissive temperature, most GMT was found in the endoplasmic reticulum of sec23 ts cells, which have defective COPII, and in the vacuole of sec21 ts cells, which have defective COPI. The C-terminal hydrophilic peptide of GMT that is exposed...

... contributed equally to this work 27 11 2003 © The Company of Biologists Limited 2004 2004 Hsp150 COPI Yeast Secretion Membrane traffic Exit of soluble and membrane proteins from the yeast endoplasmic reticulum (ER) occurs in vesicles covered with the COPII coat, which consists...

... pathways of retrograde transport. Cholera toxin contains the mammalian retrograde targeting signal KDEL and is believed to exploit the coat protein I (COPI) and KDEL receptor-dependent pathway to go from the Golgi complex to the ER. Shiga toxin, however, has no KDEL sequence to specify its inclusion...

... structures (of about 0.4 to 1 μm in diameter) with extensive budding profiles. These structures are devoid of COPII components and Golgi markers, but are enriched in COPI, the retrograde cargo ERGIC53, and the tethering protein p115. The structures appear to be able to undergo fusion with the Golgi stack...

...David J. Stephens; Nathalie Lin-Marq; Alessandra Pagano; Rainer Pepperkok; Jean-Pierre Paccaud ABSTRACT Transport of proteins between the endoplasmic reticulum and Golgi apparatus is mediated by two distinct membrane coat complexes, COPI and COPII. Genetic, biochemical and morphological data have...

...Rainer Pepperkok; J. Andrew Whitney; Marie Gomez; Thomas E. Kreis ABSTRACT Microinjection of the slowly hydrolyzable GTP analogue GTPγS or the ectopic expression of a GTP restricted mutant of the small GTPase arf1 (arf1[Q71L]) leads to the rapid accumulation of COPI coated vesicles and buds...