... is a common feature of cancer cells, and thus a potentially important therapeutic target. Here, we show that cyclin-dependent kinase (CDK)-induced replication stress, resulting from Wee1 inactivation, is synthetic lethal with mutations disrupting dNTP homeostasis in fission yeast. Wee1 inactivation leads...

... report that Cdk-dependent TRF1 phosphorylation on T371 acts as a switch to create a pool of TRF1, referred to as (pT371)TRF1, which is recruited to ALT-associated PML bodies (APBs) in S and G2 phases independently of its binding to telomeric DNA. We find that phosphorylation of T371 is essential for APB...

... Cdk5 p25 p35 β-Amyloid Excitotoxicity Cdk Cyclins Neurodegeneration Alzheimer’s disease Synaptic plasticity Learning and memory Pain signalling Neuronal migration Axonal growth Synaptogenesis Cyclin-dependent kinases (Cdks) are Ser/Thr protein kinases that associate with specific...

... that is characterized by delayed chromosome decondensation and aberrant rearrangement of the meiotic spindle. The smg7 phenotype was mimicked by exposing meiocytes to the proteasome inhibitor MG115. Together, these data indicate that SMG7 counteracts cyclin-dependent kinase (CDK) activity at the end of meiosis...

... Cdk1,the first cyclin-dependent protein kinase. (e-mail: [email protected] ; [email protected] ) © The Company of Biologists Limited 2002 2002 Cell cycle CDK Cyclin MPF Oocyte Protein kinase The two major processes common to all cell cycles are S phase, when...

... during mitosis, and its inactivation releases this silencing. The formation of PNBs is also controlled by the CDK1 pathway, whereas the recruitment of the rRNA-processing machinery appears to depend on the activity of another CDK ( Fig. 2 ). The characterization of the CDK(s) controlling this process...

... distinct functions and locations, and the specificity of cyclin-dependent kinases is mediated in part by subcellular location. Cyclin Localization Targeting CDK Yeast Yeast relies on a single cyclin-dependent kinase (CDK), Cdc28, as the sole essential cell cycle CDK ( Lorincz and Reed, 1984...

...Mary E. Miller; Frederick R. Cross ABSTRACT Cyclin-dependent kinase (CDK) activity is essential for eukaryotic cell cycle events. Multiple cyclins activate CDKs in all eukaryotes, but it is unclear whether multiple cyclins are really required for cell cycle progression. It has been argued...

... chain reaction converts the poised, but inactive, MCM complex into an enzymatically active helicase. A growing list of proteins, including Mcm10 and Cdt1, are involved in the recruitment process. Two protein kinases, the Cdc7-Dbf4 kinase (DDK) and the cyclin-dependent kinase (CDK), trigger a chain...

... cell lines were compared. Cells expressing wild-type Akt were not susceptible to the growth-arresting effect of NGF, whereas parental PC12 cells and notably cells expressing mutant Akt were so affected. Accompanying this, the expressions of CDKs and p21 WAF1 were down- and up-regulated, respectively...

...Philipp Kaldis; Zachary W. Pitluk; I. Amy Bany; Deborah A. Enke; Marisa Wagner; Edward Winter; Mark J. Solomon ABSTRACT Eukaryotic cell cycles are controlled by the activities of cyclin-dependent kinases (cdks). The major cdk in budding yeast, Saccharomyces cerevisiae , is Cdc28p. Activation...

... ) 15 06 1998 30 07 1998 © 1998 by Company of Biologists 1998 cdc25 Cell cycle Phosphorylation cdk Phosphatase Progression through the eukaryotic cell cycle involves the sequential activation of cyclin-dependent kinases (cdk) ( Nigg, 1995 ; Morgan, 1995 ). The activity...

... + in S. cerevisiae restores the G 1 arrest phenotype of cdc4 sic1 Δ double mutants. Overproduction of rum1 + in Saccharomyces cerevisiae causes a cell cycle block in G 1 with a phenotype similar to inactivation of all the Clb cyclins. Finally, we have mapped the cyclin interacting domain and Cdk...

... metazoan development. Now we can study how the crucial regulators of eukaryotic cell cycle progression, the various cyclin/cdk complexes (for a recent review see Nigg (1995) BioEssays 17, 471-480), are turned on or off during development. In Drosophila , this analysis is most advanced, in particular...

... (CDK) inhibitors block phosphorylation of cyclin-dependent kinases by the CDK-activating kinase . J. Biol. Chem . 270 , 18195 – 18197 . Baptist , M. , Dumont , J. E. and Roger , P. P. ( 1993 ). Demonstration of cell cycle kinetics in thyroid primary culture by immunostaining...

.... Affinity-depletion of cyclin dependent kinases (Cdks) from these extracts blocks the initiation of DNA replication. We define ‘S-phase promoting factor’ (SPF) as the Cdk activity required for DNA replication in these Cdk-depleted extracts. Recombinant cyclins A and E, but not cyclin B, showed significant...

... replication or spindle assembly. In many organisms, transition points seem to involve regulation of the activity of cyclin-dependent kinases (cdks) not only through their interactions with various cyclins, but also by phosphorylation-dephosphorylation cycles acting on the kinase activity of the cdks...

...Steven I. Reed; Eric Bailly; Vjekoslav Dulic; Ludger Hengst; Dalia Resnitzky; Joyce Slingerland Cyclin-dependent kinases (Cdks) control the major cell cycle transitions in eukaryotic cells. On the basis of a variety of experiments where cyclin function either is impaired or enhanced, D-type cyclins...

...G. Maridor; P. Gallant; R. Golsteyn; E. A. Nigg ABSTRACT Cyclins control the activities of cyclin-dependent protein kinases (cdks) and hence play a key role in cell cycle regulation. While B-type cyclins associate with p34 cdc2 to trigger entry into mitosis, progression through S phase requires...

... is shown in track 1. DRTFla is shifted by the anti-Rb monoclonal antibody to ○ (compare tracks 14 and 15 with 16 and 17) and ▪ indicates the PA-CA dependent complex (tracks 4 and 5). Note that the cdk-dependent complex, ▴ (tracks 8 and 9), is supershifted to • by the anti-Rb antibody (compare tracks 10...