... in C. elegans , using mutants in the kinesin light chains ( klc-1 and klc -2) and the motor subunit ( unc-116 ) expressing an ida-1::gfp transgene that labels DCVs. DCV transport in both directions was greatly impaired in an unc-116 mutant and had reduced velocity in a klc-2 mutant. In contrast...

...: Guangshuo Ou Competing interests The authors declare no competing or financial interests. 16 6 2021 18 3 2022 © 2022. Published by The Company of Biologists Ltd 2022 Summary: Mitotic PCM scaffold assembly in C. elegans embryos requires sequential domain...

... Histone modifications FRAP Hi-C C. elegans National Institute of General Medical Sciences http://dx.doi.org/10.13039/100000057 National Institutes of Health R01 GM107293 R35 GM130311 Joachim Herz Stiftung http://dx.doi.org/10.13039/100008662 #850022...

... organization during recycling remain unclear. In this study, we identified a synergistic endosomal actin assembly restricting mechanism in C. elegans involving WTS-1, the homolog of LATS kinases, which is a core component of the Hippo pathway. WTS-1 resides on the sorting endosomes and colocalizes...

... defined by the histone variant CENP-A. In the holocentric nematode C. elegans , CENP-A deposition depends on the loading factor KNL-2. Depletion of either CENP-A or KNL-2 results in defects in centromere maintenance, chromosome condensation and kinetochore formation, leading to chromosome segregation...

...Simona Abbatemarco; Alexandra Bondaz; Francoise Schwager; Jing Wang; Christopher M. Hammell; Monica Gotta ABSTRACT When exposed to stressful conditions, eukaryotic cells respond by inducing the formation of cytoplasmic ribonucleoprotein complexes called stress granules. Here, we use C. elegans...

... still are still missing a facet of the activation of ERK: detection of endogenous ERK subcellular localization in real time. In this study, we used CRISPR/Cas9-dependent genome editing to insert sequences encoding a fluorescent epitope tag into the endogenous C. elegans mpk-1 gene, resulting...

... levels is critical. In Caenorhabditis elegans , the anaphase-promoting complex/cyclosome and its co-activator FZR-1 (APC/C FZR-1 ), a ubiquitin ligase, negatively regulates centrosome assembly through SAS-5 degradation. In this study, we report the C. elegans ZYG-1 (Plk4 in humans) as a potential...

...Chunxia Wang; Yuyan Yang; Rong Fu; Yi Zhu; Huimin Zhang ABSTRACT Periodic pattern formation on the cellular and tissue scale is an important process and has been extensively studied. However, periodic pattern formation at the subcellular level still remains poorly understood. The C. elegans...

... forces during cytokinesis is not well understood. Here we show that, under mechanical stress, cortical actomyosin flow can switch polarization during cytokinesis in the C. elegans embryo. In unstressed embryos, longitudinal cortical flow contributes to contractile ring formation, while rotational...

... autophagy caused by accumulation of non-degradative autolysosomes, but very little is known about the mechanism underlying this cellular phenotype. Here, we demonstrate that loss of function of the RBG-1–RBG-2 complex ameliorates the autophagy defect in C. elegans epg-5 mutants. The suppression effect...

...Nisha Hirani; Rukshala Illukkumbura; Tom Bland; Grégoire Mathonnet; Delphine Suhner; Anne-Cecile Reymann; Nathan W. Goehring ABSTRACT The association of molecules within membrane microdomains is critical for the intracellular organization of cells. During polarization of the C. elegans zygote, both...

... in the plasma membrane and regulates endocytic transport. Despite these important functions, the structure–function relationship of this protein is poorly understood. Taking advantage of the tat-1 mutations identified by the C. elegans million mutation project, we investigated the effects of 16 single amino...

.../user-licence-1-1/ Summary: The ATPase Afg1 has evolutionarily conserved roles in both oxidative stress tolerance and mitochondrial protein homeostasis, suggesting its importance in mitochondrial quality control. Mitochondria Yeast C. elegans Protein homeostasis LACE-1...

... that have been found important for neuronal microtubule organization in C. elegans . Mutants for cdk-5 (CDK5), unc-33 (CRMP) and unc-44 (ankyrin) were found to have mixed microtubule polarity in dendrites ( Goodwin et al., 2012 ; Maniar et al., 2011 ), and in unc-116 (kinesin-1) mutants...

... are regulated at the post-transcriptional level is poorly understood. Caenorhabditis elegans hemidesmosomes (CeHDs) have a similar structure and composition to their mammalian counterparts, making C. elegans an ideal model for studying hemidesmosomes. Here, we focus on the transcription regulator CCAR-1...

... adaptive segmentation method we propose here is capable of robust extraction of the entire cell cortex, even those with inhomogeneous distribution of cortical proteins in C. elegans embryos. ImaEdge comes with a set of default parameters for automatic segmentation of the cortex that can analyze both wild...

... (srGAPs), which are highly conserved proteins that interact with the plasma membrane, regulate Rho family GTPases, and function in a wide range of cell biological processes. Slit–Robo GAP Brain evolution srGAP2 C. elegans Cell–cell adhesion Contact inhibition National Institutes...

... declare no competing or financial interests. 6 2 2017 23 10 2017 © 2017. Published by The Company of Biologists Ltd 2017 http://www.biologists.com/user-licence-1-1/ Summary: Dynein and EFF-1 have a non-autonomous function in patterning PVD dendrites in C. elegans through...

...-factor, pyrroloquinoline quinone (PQQ), activates the C. elegans DUOX protein BLI-3 to produce the ROS H 2 O 2 at the plasma membrane, which is subsequently degraded by peroxidase (MLT-7), eventually ensuring adequate levels of ROS. These ROS signals are transduced mainly by the oxidative stress...