... but briefly associates with DSBs regardless of the phase of mitosis and re-associates with DSBs in the proceeding interphase. Mechanistically, Polo kinase activity is required for its own recruitment and that of the mitotic proteins BubR1 and Bub3 to DSBs. Moreover, depletion of Rad50 severely impaired Polo...

... family of protein phosphatase 2A (PP2A) regulatory subunits (B56-PP2A), through their interaction with the mitotic checkpoint protein BUBR1, are required for proper chromosome alignment, but the underlying mechanism(s) has remained elusive. Here, we show that B56-PP2A promotes chromosome alignment...

... in Knl1, including the RVSF, SILK, MELT and KI motifs, and their role in the recruitment and regulation of the SAC proteins Bub1, BubR1, Bub3 and Aurora B. Finally, we explore how the regions of low structural complexity that characterize Knl1 are implicated in the cooperative interactions that mediate...

...Gang Zhang; Tiziana Lischetti; Jakob Nilsson ABSTRACT The Bub1–Bub3 and BubR1–Bub3 checkpoint complexes, or the Bubs, contribute to the accurate segregation of chromosomes during mitosis by promoting chromosome bi-orientation and halting exit from mitosis if this fails. The complexes associate...

...Thomas Kruse; Gang Zhang; Marie Sofie Yoo Larsen; Tiziana Lischetti; Werner Streicher; Tine Kragh Nielsen; Sara Petersen Bjørn; Jakob Nilsson Summary BubR1 is a central component of the spindle assembly checkpoint that inhibits progression into anaphase in response to improper kinetochore...

... a checkpoint-silencing role for p31 comet downstream of kinetochores. We show that p31 comet binds Mad2 when it is bound to the mitotic checkpoint complex (MCC) components BubR1 and Cdc20. Furthermore, RNAi-mediated inhibition of p31 comet results in more Mad2 bound to BubR1–Cdc20, and conversely...

... localisation to centromeres during mitosis is not required to maintain cohesion. * Author for correspondence ([email protected]) 17 12 2009 © 2010. 2010 Mitosis Kinetochore Spindle-assembly checkpoint BubR1 Cohesion The spindle-assembly checkpoint (SAC) delays...

...Sabine Elowe; Kalyan Dulla; Andreas Uldschmid; Xiuling Li; Zhen Dou; Erich A. Nigg The BubR1 checkpoint protein performs multiple functions in mitosis. We have carried out a functional analysis of conserved motifs of human BubR1 (also known as BUB1B) and demonstrate that spindle assembly checkpoint...

..., London, WC2A 3PX, UK § Present address: University of Zurich, Institute of Zoology, Winterthurerstrasse 190, 8057 Zürich, Switzerland 20 6 2007 © The Company of Biologists Limited 2007 2007 Hypoxia Spindle checkpoint Mps1 BubR1 Bub3 Cenp-C Nuf2 Oxygen plays...

... proliferation upon loss of the second APC allele. * Author for correspondence (e-mail: [email protected]) 21 9 2004 © The Company of Biologists Limited 2004 2004 Adenomatous polyposis coli Mitosis Centrosome Genetic instability Bub1 BubR1 Germline mutations...

... in mitosis in the presence of nocodazole; however, the latter failed to arrest in mitosis when treated with taxol. Immunofluorescence studies revealed that Survivin-depleted cells were unable to stably maintain BubR1 at the kinetochores in the presence of either taxol or nocodazole. Our data reveal...

...Julie C. Canman; Nitin Sharma; Aaron Straight; Katie B. Shannon; Guowei Fang; E. D. Salmon Spindle checkpoint proteins, such as Mad2 and BubR1, and the motors dynein/dynactin and CENP-E usually leave kinetochores prior to anaphase onset by microtubule-dependent mechanisms. Likewise, `chromosome...