... for membrane cycling from Golgi to ER . Cell   56 , 801 - 813 . 10.1016/0092-8674(89)90685-5 Lippincott-Schwartz , J. , Yuan , L. , Tipper , C. , Amherdt , M. , Orci , L. and Klausner , R. D. (1991). Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest...

... marker (Ng)CopB by fluorescence in Naegleria gruberi , identifying membranous structures that are disrupted by Brefeldin A treatment, consistent with Golgi localisation. Confocal and immunoelectron microscopy reveals that Ng COPB localises to tubular membranous structures. Our data identify the Golgi...

... show that human TRAPPC13 and other TRAPP subunits are critically involved in the survival response to several Golgi-disrupting agents. Loss of TRAPPC13 partially preserves the secretory pathway and viability in response to brefeldin A, in a manner that is dependent on ARF1 and the large GEF GBF1...

... 2013 15 10 2013 © 2014. Published by The Company of Biologists Ltd 2014 Golgi Arf GTPase GBF1 Brefeldin A The Golgi functions as a central organizing organelle in the secretory pathway (Farquhar and Palade, 1998 ; Emr et al., 2009). The central Golgi stack (consisting...

... substantially recovered by treatment of syntaxin-18-depleted cells with brefeldin A, a reagent that stimulates retrograde membrane flow to the ER. These results suggest that syntaxin 18 has an important role in ER subdomain organization by mediating the fusion of retrograde membrane carriers with the ER...

... 2006 2006 GBF1 ERGIC VTCs ARF-GEF Brefeldin A Protein traffic In mammalian cells, long-range transport of cargo proteins from peripheral ER exit sites (ERES) to the juxta-nuclear Golgi complex is mediated by pleiomorphic transport carriers called vesicular tubular clusters (VTCs...

...). Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67 , 601 -616. Luduena, R. F. and Roach, M. C. (1991). Tubulin sulfhydryl groups as probes and targets for antimitotic and antimicrotubule agents...

... to the plastid, with no retention in the ER, even when an ER retention/retrieval sequence was added. Incubation with brefeldin A, which blocks ER-to-Golgi trafficking by inhibiting a GTP exchange factor required for retrograde trafficking, blocked the processing of the protein. Surprisingly, it did not affect...

... are partly localized in the parasitophorous vacuole. Interestingly, the discrete formation and/or trafficking of lipid bodies are inhibited by brefeldin A and trifluoperazine. Inhibition by trifluoperazine hints at least that a de novo TAG biosynthetic pathway via phosphatidic acid contributes to lipid body...

... and Brefeldin-A-treated, non-growing, tubes. Brefeldin-A treatment established an intriguing, stable yet dynamic system of membrane aggregations in the pollen tube tip that exhibited regular movements of material with a 5-7 second period compared with the normal ∼30 second periodicity observed in growing tubes...

... secretory pathway that is insensitive to brefeldin A. Green fluorescent protein is secreted efficiently by Chinese hamster ovary cells, with 60% of synthesized proteins secreted over 8 hours. This pathway is sensitive to changes in temperature but not to factors in serum or chemicals known to affect other...

.... Glick, B. S. and Malhotra, V. (1998). The curious status of the Golgi apparatus. Cell 95 , 883 -889. Graham, T. R., Scott, P. A. and Emr, S. D. (1993). Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway. EMBO J . 12 , 869 -877...

... of the mammary secretions . In Advanced Dairy Chemistry . Vol. 1 (ed. P.F. Fox ), pp. 231 – 254 . Amsterdam : Elsevier Science . Lippincott-Schwartz , J. , Yuan , L. , Tipper , C. , Amherdt , M. , Orci , L. and Klausner , R. D. (1991). Brefeldin A’s effects...

... of brefeldin A on differentiation and implications for a role for ceramide in regulation of protein trafficking . Cell Growth Differ . 7 , 765 – 774 . Lippincott-Schwartz , J. , Yuan , L. , Tipper , C. , Amherdt , M. , Orci , L. and Klausner , R. D. (1991). Brefeldin A’s...

...: it colocalises partially with the intermediate compartment, it is found on nocodazole- induced peripheral Golgi elements and, most strikingly, on Brefeldin A-induced tubules that are moving towards microtubule plus ends. The direction of movement of membrane between the endoplasmic reticulum and Golgi apparatus...

...-linked carbohydrate by glycosylation enzymes of medial Golgi cisternae. Furthermore, after brefeldin A treatment VIP36 is segregated from resident Golgi proteins and codistributes with ER-Golgi recycling proteins. Fig. 3. VIP36 colocalisation with anterograde cargo in the 15°C intermediate...

... is inhibited under mitotic conditions . J. Biol. Chem . 271 , 17304 – 17311 . 10.1074/jbc.271.29.17304 Lippincott-Schwarz , J. , Yuan , L. , Tipper , C. , Amherdt , M. , Orci , L. and Klausner , R. D. (1991). Brefeldin A’s effects on endosomes lysosomes and the TGN...

... is involved but inhibition of the proteasome by lactacystin does not result in deglycosylation and export to the cytosol. Instead, the β chain is retained in the ER implying that extraction of the β chain from the ER membrane requires proteasome activity. Surprisingly, brefeldin A accelerates the degradation...

... and cytoplasm. The Δ N wt and the T31N mutants were exclusively cytoplasmic. The behavior of the wt and Q71L proteins was studied in detail. About 15% of wt-ARF1-GFP was bound to the Golgi. Bound wt-ARF1-GFP dissociated rapidly after addition of Brefeldin A (BFA). This process did not appear to be a consequence...

... in the culture medium; (iii) brefeldin A, an inhibitor of vesicular transport from the endoplasmic reticulum to the Golgi apparatus, decreased enzyme secretion in a time-dependent manner; (iv) whereas nocodazole, a microtubule depolymerizing agent, inhibited enzyme secretion, cytochalasin D, a microfilament...