... Attribution License ( http://creativecommons.org/licenses/by/4.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Oncogene Breast cancer Replication stress Histone modification Superresolution microscopy SiRNA...

... controls homeostasis, we investigated the relationship between basoapical polarity and resting MP in three-dimensional culture of the HMT-3522 breast cancer progression. A microelectrode technique to measure MP and input resistance reveals that the MP is raised by gap junction intercellular communication...

... amplification is sufficient, without additional pro-oncogenic alterations, to drive early tumorigenic change in a normal breast epithelial background. Centrosome amplification Rap-1 Invasion Metastases CAM assay Breast cancer Science Foundation Ireland http://dx.doi.org/10.13039...

... of metastatic cells required for colonizing distant organs, remain poorly defined. Here, we used single-cell magneto-optical capture (scMOCa) to isolate fast cells from heterogeneous human breast cancer cell populations, based on their migratory ability alone. We show that captured fast cell subpopulations...

... in breast cancer patients. To investigate whether the kinase activity of FER is essential for its oncogenic properties, we developed an ATP analogue-sensitive knock-in allele (FER ASKI ). Specific FER kinase inhibition in MDA-MB-231 cells reduces migration and invasion, as well as metastasis when...

... receptor 3 (TGFBR3) acts as an anoikis mediator through the coordination of activating transcription factor 4 (ATF4). In breast cancer tissues, TGFBR3 is progressively lost, but elevated TGFBR3 is associated with a histologic subtype characterized by cellular adhesion defects. Dissecting the impact...

..., which illustrates its use as an experimental model to study both differentiation and transformation. Comma-1D Mammary epithelium differentiation Stem cells Oncogenesis Breast cancer National Institutes of Health http://dx.doi.org/10.13039/100000002 R01GM134382 American...

.... To determine the underlying molecular mechanisms, we mapped the genome-wide localization of endogenous macroH2A1.1 in the human breast cancer cell line MDA-MB-231. We demonstrate that macroH2A1.1 specifically binds to active promoters and enhancers in addition to facultative heterochromatin. Selective knock...

... is typically reliant on bleb formation. Here we demonstrate that thrombin induces dynamic blebbing in the MDA-MB-231 breast cancer cell line and confirm that protease-activated receptor 1 (PAR1) activation is sufficient to induce this effect. Cell confinement has been implicated as a driving force in bleb...

...Natsumi Noda; Takeaki Ozawa ABSTRACT Cluster of differentiation 44 (CD44) is a single-pass transmembrane glycoprotein that is a widely distributed cell-surface adhesion molecule. CD44 undergoes ectodomain cleavage by membrane-associated metalloproteinases in breast cancer cells. Cleavage plays...

...Meera Saxena; Mizue Hisano; Melanie Neutzner; Maren Diepenbruck; Robert Ivanek; Kirti Sharma; Ravi K. R. Kalathur; Thomas R. Bürglin; Salvatore Risoli; Gerhard Christofori ABSTRACT The vast majority of breast cancer-associated deaths are due to metastatic spread of cancer cells, a process aided...

... depletion in tumour cells caused redistribution of the actin cytoskeleton and increased activity of the Rho GTPase CDC42, known for its role in actin remodelling and cell migration. In a xenograft model of breast cancer, tumour cell CD99 expression inhibited metastatic progression, and patient samples...

...Hyungjoo Kim; Seogho Son; Yunhyo Ko; Jeong Eon Lee; Sangmin Kim; Incheol Shin ABSTRACT About 70% of breast cancers overexpress estrogen receptor α (ERα, encoded by ESR1 ). Tamoxifen, a competitive inhibitor of estrogen that binds to ER, has been widely used as a treatment for ER-positive breast...

...(A) polymerase, poly(A) polymerase α (PAPOLA), in cancer, we assessed its expression in 221 breast cancer samples and found it to correlate strongly with the aggressive triple-negative subtype. Silencing PAPOLA in MCF-7 and MDA-MB-231 breast cancer cells reduced proliferation and anchorage-independent growth...

...Ang Lu; Wei Wang; Shu-Fang Wang-Renault; Brian Z. Ring; Yoshimasa Tanaka; Jun Weng; Li Su ABSTRACT Human breast cancer cells exhibit considerable diversity in the methylation status of genomic DNA CpGs that regulate metastatic transcriptome networks. In this study, we identified human Sipa1...

... – that not only affects the behavior of cells but also disrupts their ability to restore tissue integrity. While abnormal tissue properties, including increased extracellular matrix stiffness, are linked with the risk of developing breast cancer, the role and contribution of senescent MSCs to the disease...

...Maitreyi Rathod; Sushmita Chatterjee; Shruti Dutta; Rajiv Kalraiya; Dibyendu Bhattacharyya; Abhijit De ABSTRACT Chasing an intriguing biological question on the disparity of sodium iodide symporter (NIS, officially known as SLC5A5) expression and function in the clinical scenario of breast cancer...

... cell death via TRIB3 upregulation; and (ii) inhibition of invasiveness via abrogation of RAC1 SUMOylation. SUMO modification Rho-GTPase Breast cancer Autophagy Ginkgolic acid TRIB3 The post-translational addition of the small ubiquitin-related modifier (SUMO) peptide is now...

... in MDA-MB-231 breast cancer cells in which early endosomes demonstrated aberrantly enriched F-actin and accumulated the metalloproteinase MT1-MMP (also known as MMP14) upon DLC3 knockdown. Remarkably, Rab4 (herein referring to Rab4A) downregulation fully rescued the enhanced matrix degradation of TGF-β...

... with poor prognosis. Here, we report that CD151 is involved in regulation of the expression of fibroblast growth factor receptor 2 (FGFR2). Depletion of CD151 in breast cancer cells resulted in an increased level of FGFR2. Accordingly, an inverse correlation between CD151 and FGFR2 was observed in breast...