... The authors declare no competing or financial interests. © 2024. Published by The Company of Biologists Ltd 2024 Summary: A description of diffraction-limited, proximity-based, super-resolution and electron microscopy methods for visualizing interorganelle contacts. Biosensors Electron...

... imaging approaches in mice are reviewed with a focus on cancer progression and treatment, where it offers detailed insight into the dynamic interactions between cancer cells and their microenvironment in vivo . Intravital imaging Subcellular imaging FRAP FRET Biosensors Cancer Immune cells...

... molecular understanding of how intracellular signaling networks function. In particular, genetically encoded biosensors are widely used to directly visualize signaling events in living cells, and we highlight several examples of basic biosensor designs that have enabled researchers to capture the spatial...

... issues in time-lapse imaging: keeping cells alive, using labels, reporters and biosensors, and hardware and software requirements. We highlight crucial and often non-obvious problems for researchers venturing into the field and hope to inspire experts in the field and from related disciplines...