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Keywords: Biosensor
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Journal Articles
Series: REVIEW COMMONS TRANSFER
J Cell Sci (2023) 136 (10): jcs260802.
Published: 25 May 2023
.... Relocation-based biosensors for Rac and Cdc42 have not been characterized well in terms of their specificity or affinity. In this study, we identify relocation sensor candidates for both Rac and Cdc42. We compared their (1) ability to bind the constitutively active Rho GTPases, (2) specificity for Rac...
Includes: Supplementary data
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Imaging
J Cell Sci (2022) 135 (6): jcs259685.
Published: 11 March 2022
... variability. To study the dynamics and heterogeneity of ERK and Akt proteins, we employed high-content biosensor imaging with kinase translocation reporters. The kinases were activated with GPCR ligands. We observed ligand concentration-dependent response kinetics to histamine, α2-adrenergic and S1P receptor...
Includes: Supplementary data
Journal Articles
Series: REVIEW COMMONS TRANSFER
J Cell Sci (2021) 134 (17): jcs258823.
Published: 8 September 2021
... biosensors (sensors that relocalize to the native location of active Rho GTPase) seem to have been only useful in certain organisms and have not been characterized well. In this paper, we systematically examined the contribution of the fluorescent protein and Rho-binding peptides on the performance...
Includes: Supplementary data
Journal Articles
In collection:
Imaging
J Cell Sci (2017) 130 (15): 2644–2653.
Published: 1 August 2017
... against diverse cyanine family fluorogens. Our fluorescence screen identified three cell-impermeant fluorogens, each with unique emission spectra (blue, green and red) and nanomolar affinities. Most importantly, as a protein fusion tag to G-protein-coupled receptors, the antibody biosensor retained full...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (7): 1422–1433.
Published: 1 April 2015
...Masashi Maekawa; Gregory D. Fairn ABSTRACT Cholesterol is an essential component of metazoan cellular membranes and it helps to maintain the structural integrity and fluidity of the plasma membrane. Here, we developed a cholesterol biosensor, termed D4H, based on the fourth domain of Clostridium...
Includes: Supplementary data
Journal Articles
J Cell Sci (2004) 117 (14): 2879–2886.
Published: 15 June 2004
... Ras GTPase Raf Reporter Probe Biosensor Imaging Signalling Membrane Trafficking Plasma membrane Golgi Endoplasmic reticulum Endosomes Prenylation Palmitoylation Three human Ras proto-oncogenes encode small GTPases (HRas, N-Ras, K-Ras4A and K-Ras4B) that operate as binary...