... recruits Ipl1 to centromeres. Furthermore, low tension-induced Ipl1 recruitment depended on Bub1, which is known to provide a binding site for Ipl1. In contrast, Top2, which can also recruit Ipl1 to centromeres, was not required. Our results demonstrate cells are sensitive to low tension at centromeres...

... of these proteins to the kinetochore, as well as SAC activation, rely on the mitotic kinase BUB1, but the molecular mechanism by which BUB1 accomplishes this in human cells is unknown. We show that kinetochore recruitment of BUBR1 and BUB3 by BUB1 is dispensable for SAC activation. Unlike its yeast and nematode...

...Gang Zhang; Tiziana Lischetti; Jakob Nilsson ABSTRACT The Bub1–Bub3 and BubR1–Bub3 checkpoint complexes, or the Bubs, contribute to the accurate segregation of chromosomes during mitosis by promoting chromosome bi-orientation and halting exit from mitosis if this fails. The complexes associate...

...% of the cells remained in mitosis for more than 80 minutes ( Fig. 4C ). This observation implies that Cenp-F is not itself required for spindle checkpoint function, despite the fact that it binds Bub1 in a yeast two-hybrid assay ( Jablonski et al., 1998 ). Consistent with the notion that Cenp-F is not required...

... proliferation upon loss of the second APC allele. * Author for correspondence (e-mail: [email protected] ) 21 9 2004 © The Company of Biologists Limited 2004 2004 Adenomatous polyposis coli Mitosis Centrosome Genetic instability Bub1 BubR1 Germline mutations...

...Victoria L. Johnson; Maria I. F. Scott; Sarah V. Holt; Deema Hussein; Stephen S. Taylor During mitosis, the recruitment of spindle-checkpoint-associated proteins to the kinetochore occurs in a defined order. The protein kinase Bub1 localizes to the kinetochore very early during mitosis, followed...

...Stephen S. Taylor; Deema Hussein; Yunmei Wang; Sarah Elderkin; Christopher J. Morrow BUB1 is a budding yeast gene required to ensure that progression through mitosis is coupled to correct spindle assembly. Two related human protein kinases, Bub1 and BubR1, both localise to kinetochores during...