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Keywords: Axoneme
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J Cell Sci (2024) 137 (20): jcs262038.
Published: 28 May 2024
... transport (IFT)88, the small GTPase Arl13b, as well as subunits of axonemal dynein. Key parameters in sample preparation, immunolabeling and imaging acquisition are discussed to facilitate similar studies by others in the cilia research community. * Present address: Stanford Cryo-Electron Microscopy...
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In collection:
Cilia and flagella
J Cell Sci (2023) 136 (23): jcs261148.
Published: 14 December 2023
...Jovana Deretic; Ezgi Odabasi; Elif Nur Firat-Karalar ABSTRACT The primary cilium is a conserved microtubule-based organelle that is critical for transducing developmental, sensory and homeostatic signaling pathways. It comprises an axoneme with nine parallel doublet microtubules extending from...
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J Cell Sci (2021) 134 (21): jcs254227.
Published: 10 November 2021
... and fap246 axonemes with 2.3 nm and 2.2 nm resolution, respectively [Fourier shell correlation (FSC) 0.5 criterion] ( Fig. S2C ), similar to what we reported previously ( Fu et al., 2019 ). In WT axonemes, C1b is one of the largest CA projections (∼1.3 MDa). In cross-sectional view, C1b has a roughly...
Includes: Supplementary data
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In collection:
Cilia and flagella
Series: SUBJECT COLLECTION: CILIA AND FLAGELLA
J Cell Sci (2021) 134 (15): jcs258626.
Published: 3 August 2021
...Stephen M. King ABSTRACT Axonemal dyneins power the beating of motile cilia and flagella. These massive multimeric motor complexes are assembled in the cytoplasm, and subsequently trafficked to cilia and incorporated into the axonemal superstructure. Numerous cytoplasmic factors are required...
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Cilia and flagella
J Cell Sci (2018) 131 (19): jcs221648.
Published: 11 October 2018
...Ferheen Abbasi; Haruhiko Miyata; Keisuke Shimada; Akane Morohoshi; Kaori Nozawa; Takafumi Matsumura; Zoulan Xu; Putri Pratiwi; Masahito Ikawa ABSTRACT The flagellum is an evolutionarily conserved appendage used for sensing and locomotion. Its backbone is the axoneme and a component of the axoneme...
Includes: Supplementary data
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In collection:
Cilia and flagella
J Cell Sci (2018) 131 (9): jcs212324.
Published: 4 May 2018
... is necessary for the interaction of ARL13B with the axoneme both in vitro and in vivo . We further show that exogenously expressed mutants lacking the tubulin-binding G-domain (ARL13B-ΔGD) or whose GTPase domain is inactivated (ARL13B-T35N) retain ciliary localization, but fail to rescue ciliogenesis defects...
Includes: Supplementary data
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J Cell Sci (2016) 129 (8): 1547–1551.
Published: 15 April 2016
...Toshiyuki Oda; Tatsuki Abe; Haruaki Yanagisawa; Masahide Kikkawa ABSTRACT The docking complex is a molecular complex necessary for assembly of outer dynein arms (ODAs) on the axonemal doublet microtubules (DMTs) in cilia and flagella. The docking complex is hypothesized to be a 24-nm molecular...
Includes: Supplementary data
Journal Articles
In collection:
Cilia and flagella
J Cell Sci (2016) 129 (5): 943–956.
Published: 1 March 2016
...Dhivya Kumar; Crysten E. Blaby-Haas; Sabeeha S. Merchant; Richard E. Mains; Stephen M. King; Betty A. Eipper ABSTRACT Ciliary axonemes and basal bodies were present in the last eukaryotic common ancestor and play crucial roles in sensing and responding to environmental cues. Peptidergic signaling...
Includes: Supplementary data
Journal Articles
J Cell Sci (2015) 128 (7): 1294–1307.
Published: 1 April 2015
... and stabilization as well as being necessary for ciliary localization. Finally, overexpression and knockdown studies show that hSAXO1 modulates axoneme length. Taken together, our findings suggest a fine regulation of hSAXO1 localization and important roles in cilium biogenesis and function. In an attempt...
Includes: Supplementary data
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J Cell Sci (2010) 123 (4): 519–528.
Published: 15 February 2010
...Charles B. Lindemann; Kathleen A. Lesich The working mechanism of the eukaryotic flagellar axoneme remains one of nature's most enduring puzzles. The basic mechanical operation of the axoneme is now a story that is fairly complete; however, the mechanism for coordinating the action of the dynein...
Journal Articles
J Cell Sci (2008) 121 (7): 1076–1084.
Published: 1 April 2008
...Ho-Chun Wei; Janet Rollins; Lacramioara Fabian; Madeline Hayes; Gordon Polevoy; Christopher Bazinet; Julie A. Brill Axonemes are microtubule-based organelles of crucial importance in the structure and function of eukaryotic cilia and flagella. Despite great progress in understanding how axonemes...
Includes: Supplementary data
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J Cell Sci (2006) 119 (16): 3443–3455.
Published: 15 August 2006
...Carole Branche; Linda Kohl; Géraldine Toutirais; Johanna Buisson; Jacky Cosson; Philippe Bastin The Trypanosoma brucei flagellum is unusual as it is attached along the cell body and contains, in addition to an apparently conventional axoneme, a structure called the paraflagellar rod, which...
Includes: Multimedia, Supplementary data
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J Cell Sci (2006) 119 (6): 1165–1174.
Published: 15 March 2006
... apparent homologs in humans, indicating that the radial spoke has been conserved throughout evolution. The human genes encoding these proteins are candidates for causing primary ciliary dyskinesia, a severe inherited disease involving missing or defective axonemal structures, including the radial spokes...
Includes: Supplementary data
Journal Articles