... understand the ancestral and derived states of classical cadherin-mediated adhesion mechanisms. Here, using bead aggregation assays, we found that DN-cadherin EC1–EC11 and DE-cadherin EC1–EC6 exhibit Ca 2+ -dependent adhesive properties. Using high-speed atomic force microscopy (HS-AFM) imaging in solution...

... barriers. Previously, the roles of claudins during organ development have exclusively been related to their canonical functions in determining paracellular permeability. Here, we use atomic force microscopy to quantify claudin-5-dependent adhesion and find that this strongly contributes to the adhesive...

... Cancer Parallel microfiltration Cell mechanical properties Atomic force microscopy Invasion National Institutes of Health 10.13039/100000002 UL1TR000124 National Science Foundation 10.13039/100000001 CAREER DBI-1254185 Norman Cousins Center...

... mediate adhesion; however, the underlying mechanism is unknown. Here, we report atomic force microscopy imaging of DE-cadherin extracellular cadherin domains. We identified a tightly folded globular structure formed by the four N-terminal-most extracellular cadherin domains stabilized by the subsequent...

... in normal physiology and to coordinate tension during morphogenesis. Though much is known about the biological mechanisms underlying junction formation, little is known about how tissue-scale mechanical properties are established. Here, we use deep atomic force microscopy (AFM) indentation to measure...

... work is properly attributed. Single-cell force spectroscopy Atomic force microscopy ALCAM CD6 Cell adhesion Immunological synapse Membrane tethers Cell adhesion molecules (CAMs) mediate cell attachment and play important roles in tissue organization by facilitating cohesion...

... atomic force microscopy (AFM) supported this prediction. Addition of glutamate-rich PEVK domain fragments competitively inhibited the viscoelastic response in both single titin molecules and muscle fibers, indicating that PEVK domain interactions contribute significantly to sarcomere mechanics. Thus...

... respond to mechanical stimuli and assemble into large complexes remains poorly understood at the molecular level. In the past years, atomic force microscopy (AFM) has revolutionized the way in which biologists analyze cell surface proteins to molecular resolution. In this Commentary, we discuss how...

... by combined atomic force microscopy and finite element modeling . Biophys. J. 83 , 858 - 879 . Charras G. T. , Horton M. A. ( 2002b ). Single cell mechanotransduction and its modulation analyzed by atomic force microscope indentation . Biophys. J. 82 , 2970 - 2981 . Charras...

... on this matrix array could be readily discerned and quantified by a combined set-up for fluorescence and atomic force microscopy. In short, expression of the protease resulted in the generation of structural matrix defects, clearly indicated by gaps in the collagen lattice and loose fiber bundles. This key...

..., the regulation of mechanical stiffness is not yet understood. There is evidence that a possible regulator is the electrical plasma membrane potential difference. Using a novel technique that combines fluorescence-based membrane potential recordings with atomic force microscopy (AFM)-based stiffness measurements...

... and nanomechanical approach of atomic force microscopy, we visualised capsids of the ubiquitous human pathogen herpes simplex virus type 1 (HSV-1) at nano-scale resolution in physiologically relevant conditions. Simultaneously performed nano-indentation measurements on genome-containing and genome-free capsids...

... © The Company of Biologists Limited 2008 2008 Atomic force microscopy Cell adhesion Cellular interaction Dynamic force spectroscopy Extracellular matrix Molecular interaction Single molecule A broad spectrum of biological processes requires controlled cell adhesion, including...

... of tissue under remodeling. * Author for correspondence (e-mail: [email protected] ) 31 12 2007 © The Company of Biologists Limited 2008 2008 Atomic force microscopy Myofibroblast N-cadherin OB-cadherin Cytoskeleton Differentiation of fibroblasts into contractile...

... ) 11 1 2006 © The Company of Biologists Limited 2006 2006 Atomic force microscopy Mineralocorticoid Endothelial permeability Endothelial cell surface Spironolactone Cell stiffness Epithelial sodium channel Although the action of glucocorticoids is known to be widespread...

... viral DNA translocates through the comparatively small NPC channel. In the present study, the interaction of HSV-1 with NPCs was analyzed by atomic force microscopy. In addition to capsids, smaller subviral structures - most with a diameter of 35-40 nm and a length of 130-160 nm - were visualized...

... envelopment and changes induced by growth hormone. Science Vol Number 909-910 . Jang, J., Schatz, G. C. and Ratner, M. A. ( 2004 ). Capillary force in atomic force microscopy. J. Chem. Phys. 120 , 1157 -1160. Kihlmark, M., Imreh, G. and Hallberg, E. ( 2001 ). Sequential degradation...

...) was investigated using sensitive high resolution microscopy methods and flow cytometry. Confocal laser scanning microscopy (CLSM) revealed an increase of HSP60 in the mitochondria and on the surface of heat-stressed living and fixed HUVECs compared to unstressed cells. Atomic force microscopy (AFM), which has...

... of EhCaBP1, indicating that EhCaBP1 does not affect filament treadmilling. In addition, using atomic force microscopy; it was found that filaments of F-actin, polymerized in presence of EhCaBP1, were thinner. These results indicate that EhCaBP1 may be involved in dynamic membrane restructuring at the time...

... for correspondence 26 09 1994 29 11 1994 © 1995 by Company of Biologists 1995 atomic force microscopy cytoskeleton microtubule Microtubules (MTs) are fibrillar structures and ubiquitous components of the cytoskeleton of eukaryotic cells (for an overview see Hyams and Lloyd, 1994...