...Helena M. Schnell; Richard M. Walsh, Jr.; Shaun Rawson; John Hanna ABSTRACT Much of cellular activity is mediated by large multisubunit complexes. However, many of these complexes are too complicated to assemble spontaneously. Instead, their biogenesis is facilitated by dedicated chaperone proteins...

... of mammalian sperm and the paraflagellar rod (PFR) of trypanosomes. Flagellum assembly is a complex process occurring across three main compartments, the cytoplasm, the transition zone and the flagellum itself. The process begins with the translation of protein components followed by their sorting...

... heterochromatin of metazoan cells. The assembly and incorporation of lamin A/C into the lamina, as well as their various functions, are still not well understood. Here, we employed designed ankyrin repeat proteins (DARPins) as new experimental tools for lamin research. We screened for DARPins that specifically...

...Sari Tojkander; Gergana Gateva; Pekka Lappalainen Actin filaments assemble into diverse protrusive and contractile structures to provide force for a number of vital cellular processes. Stress fibers are contractile actomyosin bundles found in many cultured non-muscle cells, where they have...

...Weiming Ni; Alex H. Hutagalung; Shumin Li; Henry F. Epstein The UNC-45 family of molecular chaperones is expressed in metazoan organisms from Caenorhabditis elegans to humans. The UNC-45 protein is essential in C. elegans for early body-wall muscle cell development and A-band assembly. We show...

... ), whereas the later expression cluster contains flagella genes ( supplementary material Table S1 ). The general induction of basal body genes before flagella genes agrees with the fact that Naegleria assembles its basal bodies before it assembles its flagella ( t =55 and t =65 minutes, respectively...

... metabolism is unknown. In this study PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts. We show that all six nuclear PML isoforms exhibit individual exchange rates at NBs and identify PML V as a scaffold subunit. SP100 exchanges at least five...

.... In dystrophia muscularis ( dy ) mice bearing Lama2 mutations, myofibers and Schwann cells fail to assemble stable BLs, which are thought to be crucial for myofiber survival and Schwann cell differentiation. Here, we describe defects in a new allele of Lama2 in mice, nmf417 , in which a point mutation...

... affected by Charcot-Marie-Tooth (CMT) neuropathy type 2. We have characterized the effects of these NFL mutations on the formation of IF networks using a transient transfection system. Both mutations disrupted the self-assembly of human NFL. The Q333P mutant in the rod domain of NFL also disrupted...

...Ulrike Engel; Suat Oezbek; Ruth Engel; Barbara Petri; Friedrich Lottspeich; Thomas W. Holstein The novel protein Nowa was identified in nematocysts, explosive organelles of Hydra , jellyfish, corals and other Cnidaria . Biogenesis of these organelles is complex and involves assembly of proteins...

...Elena Kiseleva; Sandra Rutherford; Laura M. Cotter; Terence D. Allen; Martin W. Goldberg The mechanisms of nuclear pore complex (NPC) assembly and disassembly during mitosis in vivo are not well defined. To address this and to identify the steps of the NPC disassembly and assembly, we investigated...

... the commitment to complete the cell cycle made during G 1 . Characterisation of the protein factors required for initiating DNA replication is essential to understand how the cell cycle is regulated. Recent results indicate that initiation complexes assemble in multiple stages during the cell cycle. First...

... of expressed LMM cDNA indicated that charge zone complementation is not a major driving force in myosin polymerisation. Instead, it probably serves to prevent unfavourable interaction geometries. Molecular basis of myosin assembly: coiled-coil interactions and the role of charge periodicities SIMON J. ATKINSON...