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1-20 of 28
Keywords: Assembly
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Journal Articles
In collection:
Proteostasis
Journal:
Journal of Cell Science
J Cell Sci (2022) 135 (8): jcs259622.
Published: 22 April 2022
...Helena M. Schnell; Richard M. Walsh, Jr.; Shaun Rawson; John Hanna ABSTRACT Much of cellular activity is mediated by large multisubunit complexes. However, many of these complexes are too complicated to assemble spontaneously. Instead, their biogenesis is facilitated by dedicated chaperone proteins...
Journal Articles
In collection:
Cilia and flagella
Aline A. Alves, Heloisa B. Gabriel, Maria J. R. Bezerra, Wanderley de Souza, Sue Vaughan, Narcisa L. Cunha-e-Silva, Jack D. Sunter
Journal:
Journal of Cell Science
J Cell Sci (2020) 133 (10): jcs242271.
Published: 27 May 2020
... of mammalian sperm and the paraflagellar rod (PFR) of trypanosomes. Flagellum assembly is a complex process occurring across three main compartments, the cytoplasm, the transition zone and the flagellum itself. The process begins with the translation of protein components followed by their sorting...
Includes: Supplementary data
Journal Articles
Monika Zwerger, Heidi Roschitzki-Voser, Reto Zbinden, Celine Denais, Harald Herrmann, Jan Lammerding, Markus G. Grütter, Ohad Medalia
Journal:
Journal of Cell Science
J Cell Sci (2015) 128 (19): 3607–3620.
Published: 1 October 2015
... heterochromatin of metazoan cells. The assembly and incorporation of lamin A/C into the lamina, as well as their various functions, are still not well understood. Here, we employed designed ankyrin repeat proteins (DARPins) as new experimental tools for lamin research. We screened for DARPins that specifically...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2012) 125 (8): 1855–1864.
Published: 15 April 2012
...Sari Tojkander; Gergana Gateva; Pekka Lappalainen Actin filaments assemble into diverse protrusive and contractile structures to provide force for a number of vital cellular processes. Stress fibers are contractile actomyosin bundles found in many cultured non-muscle cells, where they have...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2011) 124 (18): 3164–3173.
Published: 15 September 2011
...Weiming Ni; Alex H. Hutagalung; Shumin Li; Henry F. Epstein The UNC-45 family of molecular chaperones is expressed in metazoan organisms from Caenorhabditis elegans to humans. The UNC-45 protein is essential in C. elegans for early body-wall muscle cell development and A-band assembly. We show...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2010) 123 (23): 4024–4031.
Published: 1 December 2010
...-assembly pathway . J. Cell Sci. 123 , 1414 - 1426 . Cavalier-Smith T. ( 2002 ). The phagotrophic origin of eukaryotes and phylogenetic classification of Protozoa . Int. J. Syst. Evol. Microbiol. 52 , 297 - 354 . Chang P. , Giddings T. H. Jr , Winey M. , Stearns...
Includes: Supplementary data
Journal Articles
Stefanie Weidtkamp-Peters, Thorsten Lenser, Dmitri Negorev, Norman Gerstner, Thomas G. Hofmann, Georg Schwanitz, Christian Hoischen, Gerd Maul, Peter Dittrich, Peter Hemmerich
Journal:
Journal of Cell Science
J Cell Sci (2008) 121 (16): 2731–2743.
Published: 15 August 2008
... metabolism is unknown. In this study PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts. We show that all six nuclear PML isoforms exhibit individual exchange rates at NBs and identify PML V as a scaffold subunit. SP100 exchanges at least five...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2008) 121 (10): 1593–1604.
Published: 15 May 2008
.... In dystrophia muscularis ( dy ) mice bearing Lama2 mutations, myofibers and Schwann cells fail to assemble stable BLs, which are thought to be crucial for myofiber survival and Schwann cell differentiation. Here, we describe defects in a new allele of Lama2 in mice, nmf417 , in which a point mutation...
Includes: Supplementary data
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2002) 115 (24): 4937–4946.
Published: 15 December 2002
... affected by Charcot-Marie-Tooth (CMT) neuropathy type 2. We have characterized the effects of these NFL mutations on the formation of IF networks using a transient transfection system. Both mutations disrupted the self-assembly of human NFL. The Q333P mutant in the rod domain of NFL also disrupted...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2002) 115 (20): 3923–3934.
Published: 15 October 2002
...Ulrike Engel; Suat Oezbek; Ruth Engel; Barbara Petri; Friedrich Lottspeich; Thomas W. Holstein The novel protein Nowa was identified in nematocysts, explosive organelles of Hydra , jellyfish, corals and other Cnidaria . Biogenesis of these organelles is complex and involves assembly of proteins...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2001) 114 (20): 3607–3618.
Published: 15 October 2001
...Elena Kiseleva; Sandra Rutherford; Laura M. Cotter; Terence D. Allen; Martin W. Goldberg The mechanisms of nuclear pore complex (NPC) assembly and disassembly during mitosis in vivo are not well defined. To address this and to identify the steps of the NPC disassembly and assembly, we investigated...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (2000) 113 (22): 4001–4012.
Published: 15 November 2000
...Feizhou Liu; Irving Ortiz; Alex Hutagalung; Christopher C. Bauer; Richard G. Cook; Henry F. Epstein ABSTRACT Muscle thick filaments are highly organized supramolecular assemblies of myosin and associated proteins with lengths, diameters and flexural rigidities characteristic of their source...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1999) 112 (22): 4163–4171.
Published: 15 November 1999
...Jane L. Ashworth; Vicky Kelly; Matthew J. Rock; C. Adrian Shuttleworth; Cay M. Kielty ABSTRACT The molecular mechanisms of fibrillin assembly into microfibrils are poorly understood. In this study, we investigated human fibrillin-1 carboxy-terminal processing and assembly using a recombinant...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1999) 112 (13): 2233–2240.
Published: 1 July 1999
...Gee Y. Ching; Ronald K. H. Liem ABSTRACT Type IV neuronal intermediate filament proteins consist of α-internexin, which can self-assemble into filaments and the neurofilament triplet proteins, which are obligate heteropolymers, at least in rodents. These IF proteins therefore provide good systems...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1998) 111 (3): 313–320.
Published: 1 February 1998
... tubulin-rich distal zone on intact axonemes. Localizations performed on cells regenerating flagella revealed that flagellar assembly used tyrosinated tubulin; detyrosination of the B tubule occurred during later stages of regeneration, well after microtubule polymerization. The developmental timing...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1996) 109 (6): 1415–1425.
Published: 1 June 1996
...Christine Lavoie; Joël Lanoix; Frederick W. K. Kan; Jacques Paiement ABSTRACT Smooth endoplasmic reticulum assembly was studied in a cell-free system using thin-section and freeze-fracture electron microscopy. Incubation of rat hepatocyte rough and smooth microsomes in the presence of ATP, GTP...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1996) 109 (3): 569–578.
Published: 1 March 1996
..., a nonapeptide motif previously shown to be important for regular filament formation is conserved. Recombinant trout vimentin assembles into bona fide filaments in vitro, with a temperature optimum between 18 and 24°C. Above 27°C, however, filament assembly is abruptly abolished and short filaments...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1995) 108 (11): 3541–3545.
Published: 1 November 1995
... at the distal ends of flagella, sites of axonemal assembly in vivo. Extraction of axonemes under ionic conditions known to cause the release of capping structures that link the distal ends of the axonemal microtubules to the flagellar membrane also caused the release of axonemal-bound HSP70. Taken together...
Journal Articles
Journal:
Journal of Cell Science
J Cell Sci (1995) 108 (11): 3451–3461.
Published: 1 November 1995
...Martin Goldberg; Hazel Jenkins; Terry Allen; William G. F. Whitfield; Christopher J. Hutchison ABSTRACT Xenopus egg extracts which assemble replication competent nuclei in vitro were depleted of lamin B 3 using monoclonal antibody L6 5D5 linked to paramagnetic beads. After depletion, the extracts...
Journal Articles
Stepwise assembly of initiation complexes at budding yeast replication origins during the cell cycle
Journal:
Journal of Cell Science
J Cell Sci (1995) 1995 (Supplement_19): 67–72.
Published: 1 January 1995
... the commitment to complete the cell cycle made during G 1 . Characterisation of the protein factors required for initiating DNA replication is essential to understand how the cell cycle is regulated. Recent results indicate that initiation complexes assemble in multiple stages during the cell cycle. First...
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