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Journal: Journal of Cell Science
J Cell Sci (2010) 123 (13): 2170–2178.
Published: 1 July 2010
...-A-Sepharose pelleted by centrifugation and the resulting immunodepleted lysates used in the membrane integration reaction as previously described. TRC40 (Asna-1) was similarly depleted by a 1 hour incubation of 120 μl rabbit reticulocyte lysate with 20 μl (bead volume) Protein-A-Sepharose-coated with anti...
Includes: Supplementary data