... and apoptosome formation using a split-luciferase complementary assay. Fusion of N-terminal (Nluc) and C-terminal (Cluc)-fragments of firefly luciferase to the N-terminus of human Apaf-1 was performed in HEK293 cells by using CRISPR-Cas9 technology. This resulted in a luminescent form of the apoptosome that we...

... et al., 2006 ). Surprisingly, the acid test, namely the role of Bax/Bak and the apoptosome in TNFα-induced LMP has not been performed yet. We therefore looked at TNFα/CHX-triggered LMP in WT, Bax/Bak −/− , Apaf-1 −/− , caspase-9 −/− and caspase-3/7 −/− MEFs by two means, the decrease of lysosomal...

... of Biologists Limited 2005 2005 Apoptosis Drosophila Bcl-2 family Inhibitor of apoptosis IAP Cytochrome c Caspase Apoptosome Genetic model systems have provided crucial insight into the molecular mechanisms of apoptosis. Caspases, the critical effector proteases of apoptosis, were first...

...-mediated pathways to apoptosis. Hsps can inhibit the activity of pro-apoptotic Bcl-2 proteins to prevent permeabilization of the outer mitochondrial membrane and release of apoptogenic factors. The disruption of apoptosome formation represents another mechanism by which Hsps can prevent caspase activation...