... was observed in the endoplasmic reticulum; however, this expression was not found to be related to Na + currents. SCN1A -silencing reduced MSC proliferation and delayed the cell cycle in the S phase. SCN1A silencing also suppressed the protein levels of CDK2 and AKT (herein referring to total AKT), despite...

...-kinase (PI3K)–Akt pathway. Although some studies have indicated that EGFR signaling may be controlled by signal enrichment within various membrane rafts, such as flotillin nanodomains, others have found a limited effect of disruption of these nanodomains on EGFR signaling, suggesting that specific...

... (K1055, E1056, R1064 and L1065) involved in regulating IGF1R kinase activity could be explored for designing small-molecule allosteric inhibitors for IGF1R. PI3K signaling AKT IGF1R signaling ERK MAPK pathway Allosteric inhibitors Science and Engineering Research Board http...

... also revealed that knockdown of ssc-miR-21-5p impeded the phosphorylation of AKT (herein referring to AKT1) by targeting PDCD4, which further upregulated the expression of Bax, and downregulated the levels of Bcl2 and Mmp9. Furthermore, loss of function of Mus musculus (mmu)-miR-21-5p in vivo resulted...

... laboratory via CRISPR/Cas9 technology. As shown in Fig. 4 A, these cells do not display any detectable Rictor expression as compared to wt A375 cells. While in wt A375 cells, phosphorylation of bona fide mTORC2 protein targets, such as AKT (at S473) was increased upon serum stimulation, in the Rictor KO...

...Rachel M. Furlong; Andrew Lindsay; Karen E. Anderson; Phillip T. Hawkins; Aideen M. Sullivan; Cora O'Neill ABSTRACT Akt signalling is central to cell survival, metabolism, protein and lipid homeostasis, and is impaired in Parkinson's disease (PD). Akt activation is reduced in the brain in PD...

...Catherine E. Gleason; Juan A. Oses-Prieto; Kathy H. Li; Bidisha Saha; Gavin Situ; Alma L. Burlingame; David Pearce ABSTRACT mTORC2 lies at the intersection of signaling pathways that control metabolism and ion transport through phosphorylation of the AGC-family kinases, the Akt and SGK1 proteins...

... , macropinocytic cups are organized by ‘macropinocytic patches’ in the plasma membrane. These contain activated Ras, Rac and phospholipid PIP3, and direct actin polymerization to their periphery. We show that a Dictyostelium Akt (PkbA) and an SGK (PkbR1) protein kinase act downstream of PIP3 and, together...

... phosphorylation of Akt. To test the hypothesis that CDRs increase synthesis of phosphorylated Akt1 (pAkt), we analyzed the contributions of CDRs to Akt phosphorylation in response to PDGF and EGF. CDRs appeared within several minutes of growth factor addition, coincident with a peak of pAkt. Microtubule...

... was dramatically increased following CAMSAP3 removal, leading to the upregulation of Akt proteins (also known as protein kinase B proteins, hereafter Akt) activity, which is known to promote EMT. These findings suggest that CAMSAP3 functions to protect lung carcinoma cells against EMT by suppressing Akt activity...

... required for differentiation, includeing mTOR-dependent Akt activation but not Erk1/2 activation. We identify N-myc downstream regulated gene-1 (NDRG1) as a PTPα-regulated molecule during oligodendrocyte differentiation, and distinguish Lm2 receptor-specific modes of Fyn–Akt-dependent and -independent...

...Dougall M. Norris; Pengyi Yang; James R. Krycer; Daniel J. Fazakerley; David E. James; James G. Burchfield ABSTRACT Akt is a key node in a range of signal transduction cascades and play a critical role in diseases such as cancer and diabetes. Fluorescently-tagged Akt reporters have been used...

... on the constitutive translation of HIF1α under normoxic conditions via an Akt-dependent pathway. In further support of the role of TRPC1 in EMT, its expression is closely associated with EMT- and metastasis-related genes in breast tumours, and is enhanced in basal B breast cancer cell lines. TRPC1 expression is also...

... development by increasing expression of the cell–cell adhesion molecule CsA (also known as CsaA) and causing aggregation, and this effect, as well as the inhibition of proteasome activity, is mediated by Ras and Akt proteins. Surprisingly, Ras and Akt do not affect the ability of polyphosphate to inhibit...

... ). mTOR complex 2 mediates Akt phosphorylation that requires PKCepsilon in adult cardiac muscle cells . Cell. Signal.   25 , 1904 - 1912 . 10.1016/j.cellsig.2013.05.001 Moseley , J. B. and Goode , B. L. ( 2006 ). The yeast actin cytoskeleton: from cellular function to biochemical...

... transition in oocytes is essential for reproduction. However, the mechanism for threshold establishment is unclear. In starfish oocytes, maturation-inducing hormones activate the PI3K–Akt pathway through the G βγ complex of heterotrimeric G-proteins. Akt directly phosphorylates both Cdc25 phosphatase...

...Sean M. Gross; Peter Rotwein ABSTRACT Growth factors alter cellular behavior through shared signaling cascades, raising the question of how specificity is achieved. Here, we have determined how growth factor actions are encoded into Akt signaling dynamics by real-time tracking of a fluorescent...

... has been hampered by side effects such as elevated vascular permeability. Here, we attempted to induce endothelial cell elongation without an overdose of VEGF. By screening a library of chemical inhibitors, we identified phosphatidylinositol 3-kinase (PI3K)–Akt pathway inhibitors and mammalian target...

...Nirmal Jethwa; Gary H. C. Chung; Marta G. Lete; Alicia Alonso; Richard D. Byrne; Véronique Calleja; Banafshé Larijani ABSTRACT PKB/Akt activation is a common step in tumour growth, proliferation and survival. Akt activation is understood to occur at the plasma membrane of cells in response...

... and mediates the stress granule assembly on its own, rather than indirectly, by providing balance to TORC1. Interestingly, the ribosomal localization of Rictor activates Akt, the TORC2 downstream kinase and we show here that Akt is activated upon heat stress both in animals and cell lines, in line...