... Atherosclerosis Adherens junctions Cytoskeleton British Heart Foundation http://dx.doi.org/10.13039/501100000274 FS/16/2/31739 PG/15/102/31890 Royal Veterinary College http://dx.doi.org/10.13039/100015226 Endothelial cells (ECs) are continuously exposed to haemodynamic wall...

...Chaoyu Fu; Aditya Arora; Wilfried Engl; Michael Sheetz; Virgile Viasnoff ABSTRACT The mechanisms controlling the dynamics of expansion of adherens junctions are significantly less understood than those controlling their static properties. Here, we report that for suspended cell aggregates, the time...

... Summary: Calcium signaling leads a morphogenetic process during neural rosette formation by regulating the movement of cell cytoskeletal elements. Embryonic stem cells Neural stem cell niche Neural rosette formation Calcium Neurogenesis Adherens junctions Cytoskeleton proteins...

... remaining sufficiently plastic to allow events such as cytokinesis to take place is a key biological problem. In vertebrates, the remodelling and reinforcement of adherens junctions maintains epithelial integrity during cytokinesis. The involvement of tight junctions in cell division, however, has remained...

... examined vascular permeability in EC-specific Rap1A- and Rap1B-knockout mice, cell–cell junction remodeling and EC monolayer resistivity in Rap1-deficient ECs under basal, inflammatory or elevated VEGF conditions. Deletion of either Rap1 isoform impaired de novo adherens junction (AJ) formation...

... GAP domain, we demonstrate that DLC3 activity locally regulates RhoA–ROCK signaling at and Scribble localization to adherens junctions, and is required for their functional integrity. In a 3D model of cyst development, we furthermore show that DLC3 depletion impairs polarized morphogenesis...

...Shinichiro Nakahara; Koji Tsutsumi; Takuya Zuinen; Yasutaka Ohta ABSTRACT Rho family small GTPases are essential for the formation of adherens junctions in epithelial cells. Here, we found that FilGAP (also known as ARHGAP24), a Rac-specific Rho GTPase-activating protein, promoted the formation...

... Endothelium Sphingosine-1-phosphate receptor-1 Tyrosine phosphorylation Serine phosphorylation Endothelial barrier function Adherens junctions Sphingosine-1-phosphate receptor-1 (S1PR1) is a high affinity G-protein-coupled receptor (GPCR) ligated by the lipid mediator S1P ( Hla, 2004 ; Spiegel...

...Stéphanie Le Bras; Roland Le Borgne ABSTRACT Epithelia are compact tissues comprising juxtaposed cells that function as mechanical and chemical barriers between the body and the environment. This barrier relies, in part, on adhesive contacts within adherens junctions, which are formed...

...Stella Alimperti; Hui You; Teresa George; Sandeep K. Agarwal; Stelios T. Andreadis ABSTRACT Although soluble factors, such as transforming growth factor β1 (TGF-β1), induce mesenchymal stem cell (MSC) differentiation towards the smooth muscle cell (SMC) lineage, the role of adherens junctions...

...Andrew R. Harris; Alicia Daeden; Guillaume T. Charras ABSTRACT Adherens junctions and desmosomes integrate the cytoskeletons of adjacent cells into a mechanical syncitium. In doing so, intercellular junctions endow tissues with the strength needed to withstand the mechanical stresses encountered...

... RPTPα (human gene name PTPRA ) is recruited to epithelial adherens junctions at the time of cell–cell contact, where it is in molecular proximity to E-cadherin. RPTPα is required for appropriate cadherin-dependent adhesion and for cyst architecture in three-dimensional culture. Loss of RPTPα impairs...

... by The Company of Biologists Ltd 2014 Actin cytoskeleton Actin treadmilling Adherens junctions Actomyosin Microtubule dynamics Cell polarization During normal development and in pathological contexts (such as cancer), opportunistic cell–cell contacts either are self-reinforcing, leading...

...Hui You; Roshan M. Padmashali; Aishwarya Ranganathan; Pedro Lei; Nomeda Girnius; Roger J. Davis; Stelios T. Andreadis Summary We demonstrate that c-Jun N-terminal kinase (JNK) responds to substrate stiffness and regulates adherens junction (AJ) formation in epithelial cells in 2D cultures and in 3D...

... form adherens junctions (AJs) but completely lack tight junctions (TJs) – fully activated SRF. By contrast, AGS gastric adenocarcinoma epithelial cells, which form TJs but are deficient in E-cadherin, and therefore also in AJs, failed to activate SRF. The introduction of wild-type E-cadherin in AGS...

... polarization. Adherens junctions (AJs) and VE-cadherin (VEC, encoded by CDH5 ) are required for endothelial apicobasal polarity in vitro and during embryonic development. Silencing of CDH5 gene expression leads to abrogation of endothelial polarity accompanied by strong alterations in lumenal structure. VEC co...

...Xiao Peng; Laura E. Cuff; Cort D. Lawton; Kris A. DeMali Vinculin was identified as a component of adherens junctions 30 years ago, yet its function there remains elusive. Deletion studies are consistent with the idea that vinculin is important for the organization of cell-cell junctions. However...

..., ischemia and diabetes – which leads to severe, and sometimes fatal, organ dysfunction. Endothelial permeability is regulated in part by the dynamic opening and closure of cell-cell adherens junctions (AJs). In endothelial cells, AJs are largely composed of vascular endothelial cadherin (VE-cadherin...

... MCF7 epithelial cells disrupts adherens junctions, as manifested by the significantly decreased localization of E-cadherin and associated proteins to cell-cell contacts. Expression of mouse Dia1, which is insensitive to the human Dia1-specific shRNA, rescued the junctional integrity. Coexpression...

.... Exo84 activity is essential for the apical localization of the Crumbs transmembrane protein, a key determinant of epithelial apical identity. Adherens junction proteins become mislocalized at the cell surface in Exo84 mutants in a pattern characteristic of defects in apical, but not basolateral...