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J Cell Sci (2017) 130 (13): 2185–2195.
Published: 01 July 2017
... motor to traffic to the nuclear membrane and deliver their genome through nuclear pore complexes (NPCs) into the nucleus. How virus particles (virions) or cellular cargo are transferred from microtubules to the NPC is unknown. Here, we analyzed trafficking of incoming cytoplasmic adenoviruses by single...
Includes: Supplementary data
Tanya D. Russell, Jerome Schaack, David J. Orlicky, Carol Palmer, Benny Hung-Junn Chang, Lawrence Chan, James L. McManaman
J Cell Sci (2011) 124 (19): 3247–3253.
Published: 01 October 2011
... in adipocytes . J. Biol. Chem. 282 , 5726 - 5735 . He T. C. , Zhou S. , da Costa L. T. , Yu J. , Kinzler K. W. , Vogelstein B. ( 1998 ). A simplified system for generating recombinant adenoviruses . Proc. Natl. Acad. Sci. USA 95 , 2509 - 2514 . Hollmann K...
J Cell Sci (2006) 119 (12): 2621–2631.
Published: 15 June 2006
... to be understood. Adenovirus Upstream binding factor Nucleolus Replication Adenoviruses contain a linear dsDNA genome of approximately 36 kb that is replicated in the nucleus of the host cell ( Liu et al., 2003 ; Shenk, 2001 ). The adenovirus DNA polymerase catalyses the covalent linkage of 5...
J Cell Sci (2004) 117 (16): 3579–3592.
Published: 15 July 2004
... interest in developing adenoviruses as defective vectors to carry and express foreign genes for research and therapeutic purposes ( Yeh and Perricaudet, 1997 ; Benihoud et al., 1999 ; Mountain, 2000 ). These vectors present several advantages: (1) their genomes are easily manipulated in vitro, (2) give...
Jacques-Antoine Haefliger, Thomas Tawadros, Laure Meylan, Sabine Le Gurun, Marc-Estienne Roehrich, David Martin, Bernard Thorens, Gérard Waeber
J Cell Sci (2003) 116 (8): 1463–1469.
Published: 15 April 2003
.../JIP-1 content was experimentally modulated in β cells using adenovirus gene transfer. We used recombinant adenoviruses containing the complete IB1/JIP-1 cDNA inserted in the sense or antisense orientation(Ad-sIB1 and Ad-asIB1, respectively). A control adenovirus encoding GFP was used as control...
J Cell Sci (2003) 116 (1): 73–79.
Published: 01 January 2003
... gene in 3T3L1 adipocytes on (pro)insulin release and glucose uptake. The human preproinsulin gene was transferred into 3T3L1 adipocytes by infecting the cells with recombinant adenovirus Adex1CA human preproinsulin. Immunocytochemical studies showed that (pro)insulin is associated with vesicular...
Thomas Tawadros, Andrea Formenton, Jean Dudler, Nancy Thompson, Pascal Nicod, Hans-Jürg Leisinger, Gérard Waeber, Jacques-Antoine Haefliger
J Cell Sci (2002) 115 (2): 385–393.
Published: 15 January 2002
...-Jun expression in urothelium of dilated bladder infected with the overexpressing IB1/JIP-1 recombinant adenovirus (adCMV-sIB1/JIP-1). IB1/JIP-1 content was experimentally modulated in urothelium using a recombinant adenovirus gene transfer approach. We used recombinant adenoviruses containing...
J Cell Sci (2001) 114 (17): 3147–3153.
Published: 01 September 2001
...°, 40 cycles of 1 minute at 94°C, 2 minutes at 58°C (R26R) or 60°C (Cre) and 3 minutes at 72°C, followed by 7 minutes at 72°C. We would like to thank Drs Abdelative, Schneider and Soriano for providing the recombinant adenoviruses, CAG-CATZ mouse line and R26R mice, respectively and Shirley...