... to regulate the precise intracellular patterning of SNAP25. * Author for correspondence ( Luke.Chamberlain@ed.ac.uk ) 24 11 2010 © 2011. 2011 SNAP25 Palmitoylation Acylation Intracellular trafficking Intracellular-membrane-fusion events in eukaryotes are regulated by members...

... of lateral heterogeneity in plasma membranes that serve to sequester and organize specialized protein complexes. Consistent with this, a dually acylated Ca 2+ sensor that is concentrated in the flagellum is found in detergent-resistant membranes and mislocalizes if the lipid rafts are disrupted. Detergent...

... contributes to the sorting of proteins to the eukaryotic flagellum. African trypanosomes are protozoan pathogens that express a family of unique Ca 2+ -binding proteins, the calflagins, which undergo N-terminal myristoylation and palmitoylation. The localization of calflagins depends on their acylation status...

... of palmitate-deficient Wingless to the plasma membrane does not rescue signalling activity. Therefore, palmitate at C93 has a dual role: in secretion and signalling. From our analysis of [S239A]Wingless, which lacks a conserved residue shown to be acylated in Wnt3a, we infer that palmitoleic acid...

... of a yeast myosin-V mediates its attachment to vacuole membranes and sites of polarized growth. Proc. Natl. Acad. Sci. USA 95 , 14799 -14804. Denny, P. W., Gokool, S., Russell, D. G., Field, M. C. and Smith, D. F. ( 2000 ). Acylation-dependent protein export in Leishmania. J. Biol. Chem. 275...

... an unconventional secretory pathway. Exported HASPB remains anchored in the outer leaflet of the plasma membrane mediated by myristate and palmitate residues covalently attached to the N-terminal SH4 domain of HASPB. HASPB targeting to the plasma membrane depends on SH4 acylation that occurs at intracellular...

...) anchors are found at the C terminus of a wide range of cell surface proteins, and may endow the cell with the ability to release them in a controlled fashion via specific phospholipases. We have concentrated on the direct attachment (acylation) of long-chain fatty acids (myristate, C 14:0 and palmitate, C...