... to enhance vesicle fission by modifying the lipid environment. PKD also phosphorylates the actin regulatory protein cortactin at S298 to impair synergistic actin polymerization. We here report additional functions for PKD2 (also known as PRKD2) and cortactin in the regulation of actin polymerization during...

...-1-1/ Summary: The NK activating receptor NKG2D–DAP10 recruits EVL to the cytotoxic synapse via a Grb2–VAV1 signaling node to promote F-actin generation, target cell adhesion and the development of cell-mediated cytotoxicity. NK cell Cytotoxicity Actin polymerization EVL...

... and are specifically localized in the space between the chloroplast and the plasma membrane, and is called chloroplast-actin. In addition, several of the genes that encode proteins that are involved in chloroplast-actin polymerization or maintenance have been identified. In this Review, we discuss the mechanisms...

... adhesion during C. elegans morphogenesis.   J. Cell Biol.   191 , 761 – 769 . 10.1083/jcb.201005082 10 03 2013 © 2013. Published by The Company of Biologists Ltd 2013 CIP4 F-BAR Lamellipodia Actin polymerization Lamellipodia and filopodia are dynamic actin-based...

... nF yielded a field decay time constant of RC  = 42 µseconds for each pulse. Our standard procedure consisted of three pulses at intervals of 3 seconds. Actin polymerization Cell repair Diffusion Electric lipid distortion Fusion pores Membrane fusion Membrane fusion in lipid...

... polymerization were attenuated by overexpression of BCR in hippocampal neurons. Knockdown of BCR using specific shRNAs increased the dendritic arborization as well as actin polymerization. The number of dendrites in null mutant BCR −/− mice was considerably increased compared with that in wild-type mice. We...

... discuss how cell surface dynamics depend on the orchestration of the cytoskeleton and the plasma membrane by Rho GTPases. * Author for correspondence ( meldolesi.jacopo@hsr.it ) © 2012. Published by The Company of Biologists Ltd 2012 Actin polymerization Exocytosis Endocytosis Membrane...

... polymerization of actin filaments and their association with α-actinin. Only then is a local increase in integrin β3 levels apparent in the podosome ring domain. Thus, local actin polymerization in cortactin- and paxillin-rich locations nucleates podosome assembly before the local accumulation of β3 integrin. We...

... studies have revealed several mechanisms that Abl-family kinases use to mediate these effects. Abl-family kinases stimulate actin polymerization through the activation of cortactin, hematopoietic lineage cell-specific protein (HS1), WASp- and WAVE-family proteins, and Rac1. They also attenuate cell...

...Hiroaki Hirata; Hitoshi Tatsumi; Masahiro Sokabe We examined the effects of mechanical forces on actin polymerization at focal adhesions (FAs). Actin polymerization at FAs was assessed by introducing fluorescence-labeled actin molecules into permeabilized fibroblasts cultured on fibronectin. When...

..., inhibition of myosin II induced the outgrowth of F-actin-rich surface protrusions. The development of these protrusions was abrogated after inhibition of F-actin polymerization or of phospholipase C (PLC) activity, as well as after overexpression of a dominant-negative ADF/cofilin. Surface protrusions were...

..., A. and Parkos, C. A. ( 2005 ). Differential roles for actin polymerization and a myosin II motor in assembly of the epithelial apical junctional complex. Mol. Biol. Cell 16 , 2636 -2650. Ivanov, A. I., McCall, I. C., Babbin, B., Samarin, S. N., Nusrat, A. and Parkos, C. A. ( 2006 ). Microtubules...

...Ariadna Mendoza-Naranjo; Christian Gonzalez-Billault; Ricardo B. Maccioni A number of psychiatric and neurodegenerative disorders, such as Alzheimer's disease, are characterized by abnormalities in the neuronal cytoskeleton. Here, we find that the enhancement in actin polymerization induced...

...Ina Weisswange; Till Bretschneider; Kurt I. Anderson Actin polymerization drives many cellular events, including endocytosis, pathogen rocketing, and cell spreading. Force generation and polymerization regulation are intimately linked where an actin meshwork attaches to, and pushes against...

... elusive. RNA interference-mediated suppression of cortactin, an F-actin binding protein that promotes Arp2/3 complex-mediated actin polymerization, effectively blocked transferrin uptake. Depletion of cortactin in brain cytosol inhibited formation of clathrin-coated vesicles by 70% as analyzed in a cell...

... with increased invasiveness. Cortactin can regulate actin dynamics directly via its N-terminal half, which can bind and activate the Arp2/3 complex. The C-terminal portion of cortactin, however, is thought to have limited function in its regulation of the actin polymerization machinery. In this report, we...

... cluster, tyrosine-phosphorylated proteins accumulate, and intense actin polymerization occurs. We show that actin polymerization is controlled by the small GTPases Cdc42 and Rac, which become activated upon infection. Expression of dominant negative forms of these GTPases inhibits C. caviae entry...

... with N-WASP, a potent activator of the Arp2/3 complex that forms a critical part of the actin polymerization machinery. Syndapin oligomers can thereby couple bursts of actin polymerization with the vesicle fission step involving dynamins. This allows newly formed vesicles to move away from the donor...

... 2004 © The Company of Biologists Limited 2004 2004 Actin Nemaline myopathy Actin polymerization Protein folding Myopathy Actin mutations We did not express the non-folding mutants from category I but we did express representative mutants from categories II, III and IV...

... linkages between the actin polymerization machinery and adhesion molecules are also emerging in the context of cell-cell adhesion. E-cadherin colocalizes and co-immunoprecipitates with the Arp2/3 complex( Kovacs et al., 2002 ). This interaction localizes actin polymerization to sites of cadherin engagement...