... (unpaired two-tailed t -test). Scale bars: 10 μm (A), 5 μm (E), 2 μm (D). To monitor and manipulate actin dynamics on a cell-by-cell basis and more specifically in lateral protrusions, we looked for an actin regulatory protein that might act in these structures. Branched actin filament networks...

... FA330/9-2 GRK2223 Ena/VASP proteins Actin dynamics Cell adhesion Cell migration Protrusion Summary: A summary of the history and progress in our understanding of Ena/VASP family proteins, which constitute crucial members of actin filament polymerases operating in various cell types...

... degradation ( Stylli et al., 2009 ). In sarcoma cells, Nck2 binds to phosphorylated AFAP1L1 to enhance formation of invadopodia ( Tie et al., 2016 ). Nck promotes invasion of breast carcinoma cells and metastasis by regulating invadopodia actin dynamics and enhancing matrix metalloproteinase 14 (MMP14) cell...

... Immunological synapse Dynein Actin dynamics When T cells engage antigen-presenting cells they form a specialized contact site known as the immunological synapse. Classically, the synapse can be described in terms of concentric zones of receptors, adhesion proteins and cytoskeletal elements ( Monks et...

... to allow microtubule protrusion. Actin dynamics Axon branching Cofilin Twinstar γ-neurons Mushroom body Neuronal remodeling The limited regeneration of injured adult neurons within central nervous systems is due to a combination of inhibitory environments ( Tedeschi and Bradke, 2017...

... their target attachment site in Drosophila . Filopodia Integrin signaling Actin dynamics Muscle Drosophila Muscle development involves similar phases of differentiation and movement in Drosophila and vertebrate embryos ( Schweitzer et al., 2010 ). Drosophila somatic muscles display...

... Actin dynamics ADF Actin filament severing TALEN The actin cytoskeleton undergoes constant assembly and disassembly and forms distinct structures within cells to fulfill diverse physiological cellular functions. Pollen tube growth depends on a dynamically remodeled actin cytoskeleton...

... et al., 2015 ). In various cellular contexts, actin dynamics supplements the energy required for formation of clathrin-coated vesicles under high membrane tension ( Aghamohammadzadeh and Ayscough, 2009 ; Boulant et al., 2011 ; Kaur et al., 2014 ). However, actin-dependent clathrin-mediated...

... on actin and actin-related functions relies on the visualization of actin structures without interfering with the cycles of actin polymerization and depolymerization that underlie cellular actin dynamics. In this Cell Science at a Glance and the accompanying poster, we briefly evaluate the different...

...-transferase (GST)-fold, can induce ion currents in artificial membranes and show oxidoreductase activity in vitro , but there is no convincing evidence of CLICs having such activities in vivo. Recent studies have revealed a role for CLIC proteins in Rho-regulated cortical actin dynamics as well as vesicular...

...Guo-Lei Zhou; Haitao Zhang; Huhehasi Wu; Pooja Ghai; Jeffrey Field ABSTRACT Cell signaling can control the dynamic balance between filamentous and monomeric actin by modulating actin regulatory proteins. One family of actin regulating proteins that controls actin dynamics comprises cyclase...

...-associatied substrate Cas BCAR1 p130Cas Src family kinase SFK Actin dynamics Focal adhesion kinase FAK The Crk-associated substrate (Cas, p130Cas or BCAR1) is a prominent member of the Cas family of multidomain scaffolding proteins that are phosphorylated by major tyrosine kinases of the Abl...

... of the qualitative results of the calibration experiments as well as most of the quantitative results. Table 1. A comparison of the results from the vertex model of tissue mechanics and experimental data Actin dynamics Cell death Myosin Apoptosis Epithelia Extrusion RhoGTPase...

.... A. , Tüzel   E. , Vidali   L. , Bezanilla   M. ( 2011 ). Actin interacting protein1 and actin depolymerizing factor drive rapid actin dynamics in Physcomitrella patens.   Plant Cell   23 , 3696 – 3710 . 10.1105/tpc.111.090753 Bachmann   C. , Fischer   L. , Walter   U. , Reinhard...

... Attribution License ( http://creativecommons.org/licenses/by/3.0 ), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. Actin dynamics Arp2/3 Astrocyte Central nervous system CNS Morphology Brain injury...

... and Richardson, 2003 ; Pagliarini and Xu, 2003 ). Functions of candidate proteins in actin dynamics are depicted in Fig. 1A . The previously established enrichment of matrix metalloprotease 1 ( mmp1 ) ( Uhlirova and Bohmann, 2006 ) and downregulation of the protease inhibitor Reck mRNAs ( Srivastava et al...

... ). Quantitative fluorescent speckle microscopy of cytoskeleton dynamics.   Annu. Rev. Biophys. Biomol. Struct.   35 , 361 – 387 . 10.1146/annurev.biophys.35.040405.102114 Delorme   V. , Cayla   X. , Faure   G. , Garcia   A. , Tardieux   I. ( 2003 ). Actin dynamics is controlled...

.... , Mooren   O. L. , Cooper   J. A. ( 2010 ). Actin dynamics and endocytosis in yeast and mammals . Curr. Opin. Biotechnol.   21 , 604 - 610 . Giardini   P. A. , Fletcher   D. A. , Theriot   J. A. ( 2003 ). Compression forces generated by actin comet tails on lipid vesicles . Proc...

... Biology and Development, Rockefeller University, New York, NY 10065, USA ‡ Author for correspondence ( benny.geiger@weizmann.ac.il ) 17 11 2011 © 2012. 2012 Actin dynamics Actin polymerization Integrin adhesion Osteoclast Podosome Sealing zone Podosomes are dynamic...

... role of srGAP3 on actin dynamics, specifically on lamellipodia formation. We show that the F-BAR domain localizes srGAP3 to the leading edge of cellular protrusions whereas the SH3 domain is important for focal adhesion targeting. We report on a novel srGAP3 interaction partner, lamellipodin, which...