... transendothelial macroaperture surface area. Polyunsaturated phospholipids Docosahexaenoic acid DHA Membrane fusion Large-scale membrane dynamics Endothelium Transendothelial cell macroaperture Bacterial toxin Actin cytoskeleton Inhibition of the small GTPase RhoA by bacterial ExoC3-like...

... depletion in tumour cells caused redistribution of the actin cytoskeleton and increased activity of the Rho GTPase CDC42, known for its role in actin remodelling and cell migration. In a xenograft model of breast cancer, tumour cell CD99 expression inhibited metastatic progression, and patient samples...

... (protocols 140/2014, 043/2016). In conclusion, this study shows that knockdown of RACK1 in MCs results in a depolymerization and redistribution of the actin cytoskeleton that leads to gross morphological changes, increased release of preformed mediators and alterations in Ca 2+ signaling...

... cytokines with chemokine-like functions that coordinate a wide spectrum of biological activities, such as migration. Here, we biotin-tagged intracellular MIF/D-DT in vivo to identify important cytosolic interactors and found a plethora of actin cytoskeleton-associated proteins. Although the receptor complex...

.... , Blundell , K. , Longtine , M. S. , Chow , C. J. , Adames , N. , Pringle , J. R. , Peter , M. and Boone , C. ( 1997 ). Bni1p, a yeast formin linking cdc42p and the actin cytoskeleton during polarized morphogenesis . Science 276 , 118 - 122 . 10.1126/science.276.5309.118...

.... , Wong , C. C. , Bailey , A. O. , Yates , J. R. , Mogilner , A. , Zebroski , H. and Kashina , A. ( 2006 ). Arginylation of beta-actin regulates actin cytoskeleton and cell motility . Science   313 , 192 - 196 . 10.1126/science.1129344 Kijima , S. T. , Hirose , K...

... remodeling, giant cells in immune response, and how do they compare with known fusogens? Second, how do the actin cytoskeleton and fusogens coordinate in various cell–cell fusion events? Third, are there additional types of lipids that modulate cell–cell fusion? Finally, once a nano-sized fusion pore has...

... characteristics, needs careful reconsideration. Actin cytoskeleton Jurkat cells Immune synapse Primary T-cells A healthy actin cytoskeleton is crucial for T-cell function. The actin machinery integrates T-cell receptor (TCR) signalling and biophysical mechanisms to coordinate the activation of T...

...Salma Jalal; Shidong Shi; Vidhyalakshmi Acharya; Ruby Yun-Ju Huang; Virgile Viasnoff; Alexander D. Bershadsky; Yee Han Tee ABSTRACT Actin cytoskeleton self-organization in two cell types, fibroblasts and epitheliocytes, was studied in cells confined to isotropic adhesive islands. In fibroblasts...

... on sub-cellular structures, such as the adherens junctions and associated actin cytoskeleton help to elucidate how cells within the monolayer can adopt different roles, e.g. leader and follower ( Khalil and Friedl, 2010 ), establish local polarity with differential distribution of proteins and asymmetric...

.... Actin cytoskeleton Actin-myosin contraction Active gel Cell cortex Myosin end-dwelling Polarity sorting Cells have the remarkable ability to actively deform themselves to drive vital processes such as cell division, cell migration and multicellular tissue dynamics. The main determinant...

... not extend into lamellipodia ( Fig. 7 E). To investigate the role of CD2AP in actin cytoskeleton and cell migration relative to PAWS1, we generated CD2AP-knockout U2OS cells (CD2AP −/− ) by CRISPR/Cas9, targeting the exon 3 of the CD2AP gene. The loss of CD2AP protein in the isolated clone of U2OS...

... of adhesion complexes ( Ezratty et al., 2005 ) and regulating the actin cytoskeleton ( Chang et al., 2008 ). Microtubule organization in motile cells is variable, and depending on the cell type can be polarized to the front or rear of the cell ( Etienne-Manneville, 2013 ; Yoo et al., 2012 ; Xu et al., 2005...

...Klemens Rottner; Jan Faix; Sven Bogdan; Stefan Linder; Eugen Kerkhoff ABSTRACT The actin cytoskeleton and associated motor proteins provide the driving forces for establishing the astonishing morphological diversity and dynamics of mammalian cells. Aside from functions in protruding and contracting...

... (SORBS) proteins reveals that vinexin-α and CAP regulate vinculin behavior depending on the stiffness of the extracellular matrix, while ArgBP2 plays a role in generating greater contractile forces. Vinexin CAP ArgBP2 Focal adhesion Actin cytoskeleton Mechanotransduction Extracellular...

... ). Fibroblast growth factor-2 mediates pressure-induced hypertrophic response . J. Clin. Invest.   104 , 709 - 719 . 10.1172/JCI7315 Shankar , J. and Nabi , I. R. ( 2015 ). Actin cytoskeleton regulation of epithelial mesenchymal transition in metastatic cancer cells . PLoS ONE   10...

...Riko Hatakeyama; Keiko Kono; Satoshi Yoshida ABSTRACT The plasma membrane (PM) is frequently challenged by mechanical stresses. In budding yeast, TORC2-Ypk1/Ypk2 kinase cascade plays a crucial role in PM stress responses by reorganizing the actin cytoskeleton via Rho1 GTPase. However, the molecular...

... conserved functions in integrin receptor recycling and lysosome neutralization. Drosophila WASH Actin cytoskeleton Cell adhesion Lysosome neutralization Macrophages Arp2/3 Wiskott-Aldrich syndrome V-ATPase Integrin The Wiskott-Aldrich syndrome protein (WASP) family of nucleation...

..., a process that is regulated by the actin cytoskeleton and which can be detected using a FRET-based tension sensor for vinculin ( Grashoff et al., 2010 ). Expressing this vinculin tension sensor in B16F1 cells showed that vinculin in Rap1V12-expressing cells, and at the leading edge of control cells...

... properties. eEF1A Amino acid regulation Vacuole, Genome stability Spindle pole body Actin cytoskeleton Oncogene Trehalose The eukaryotic translation elongation factor 1A (eEF1A) is one of the most abundant proteins in the cell, accounting for between 3 and 10% of all soluble protein...