... anti-Myc (06-549, Millipore, Billerica, MA); chicken polyclonal anti-GFP (ab13970, Abcam, Cambridge, MA); rat monoclonal anti-HA (3F10, Roche, Indianapolis, IN); mouse monoclonal antibodies against β-tubulin, α-tubulin, acetylated tubulin and tyrosinated tubulin (TUB 2.1, B-5-1-2, 6-11B-1 and TUB-1A2...

... about the distribution of acetylated microtubules in flea-beetle spermatocytes at all stages of the first meiotic division, extending the observations of Wilson and Forer (Wilson and Forer, 2000). In general, microtubules that contain acetylated tubulin are more stable than others and are `older...

... Kinetochore microtubule Spindle Spindle matrix Metaphase Acetylated tubulin Poleward flux of tubulin molecules in kinetochore microtubules of metaphase cells was demonstrated as poleward movement of locally uncaged fluorescent tubulin in vivo ( Mitchison, 1989 ; Mitchison and Salmon, 1992 ; Zhai...

... Mitosis Xenopus egg extract In vitro spindle assembly Microtubule dynamics Microtubule-associated protein Acetylated tubulin Immunodepletion The accurate assembly of the mitotic spindle is essential for the segregation of sister chromatids during cell division, and requires complex...

... by Company of Biologists 1998 Apoptosis Programmed cell death tau1 PHF1 Neuron PC12 NGF Protein phosphatase 2A Okadaic acid Microtubule Acetylated tubulin Alzheimer’s disease Apoptosis can be divided into three sequential phases ( Jacobson et al., 1994 ; Greenlund et al., 1995...

... chromosome motion spindle fibre disassembly acetylated tubulin Anaphase kinetochore microtubule (kMT) disassembly forms a cornerstone of many models of chromosome motion. Various studies have attempted to determine the site of kMT disassembly in anaphase by marking the kMTs and observing how...

... © 1993 by Company of Biologists 1993 tyrosinated tubulin acetylated tubulin microtubules microtubule dynamics axon In a previous study, we demonstrated that individual axonal microtubules (MTs) are composite, consisting of two distinct domains that differ in their content of post...

... by their reflection of the epi-illumination (arrowheads). They are all found just clockwise of a rootlet. The anti-tubulin antibody is 3A5. Bar, 5 μ m. Chlamydomonas asymmetry basal bodies acetylated tubulin eyespot uni mutants © 1989 by Company of Biologists 1989 23 04 1989 12...

...-lived. Prophase cells displayed no acetylated tubulin staining, indicating that the maximum lifetime of cytoplasmic acetylated domains was one cell cycle. The results show that in this cell line only limited domains of stable MTs usually become acetylated, and that the turnover of these domains...