...Sabrina M. Holland; Gianluca Gallo ABSTRACT The regulation of the intracellular level of ATP is a fundamental aspect of bioenergetics. Actin cytoskeletal dynamics have been reported to be an energetic drain in developing neurons and platelets. We addressed the role of actin dynamics in primary...

...Moumita Basu; Purnima Gupta; Ananya Dutta; Kuladip Jana; Anindita Ukil ABSTRACT Intracellular survival of Leishmania donovani demands rapid production of host ATP for its sustenance. However, a gradual decrease in intracellular ATP in spite of increased glycolysis suggests ATP efflux during...

...Masak Takaine; Masaru Ueno; Kenji Kitamura; Hiromi Imamura; Satoshi Yoshida ABSTRACT Adenosine triphosphate (ATP) is a main metabolite essential for all living organisms. However, our understanding of ATP dynamics within a single living cell is very limited. Here, we optimized the ATP-biosensor...

... and cell survival with the nutrient environment of cells. The poor vasculature and nutritional stress experienced by cells in solid tumours raises the question: how do they assimilate sufficient nutrients to survive? Here, we show that human and fission yeast cells import ATP and AMP from their external...

... diphosphate kinase, thereby locally providing ATP to fuel the actomyosin machinery. These results provide a novel mechanism for the regulation of actomyosin contractility at endothelial junctions and might have broader implications in biological contexts such as angiogenesis, collective migration and tissue...

...Yuan Chi; Kun Gao; Kai Li; Shotaro Nakajima; Satoru Kira; Masayuki Takeda; Jian Yao ABSTRACT Connexin hemichannels regulate many cell functions. However, the molecular mechanisms involved remain elusive. Hemichannel opening causes loss of ATP, we therefore speculated a potential role for AMPK...

...Chen Xu; Junhua Zhang; Doina M. Mihai; Ilyas Washington ABSTRACT Sunlight is the most abundant energy source on this planet. However, the ability to convert sunlight into biological energy in the form of adenosine-5′-triphosphate (ATP) is thought to be limited to chlorophyll-containing chloroplasts...

...Matthew W. Grol; Alexey Pereverzev; Stephen M. Sims; S. Jeffrey Dixon Summary The primordial intercellular signaling molecule ATP acts through two families of cell-surface P2 receptors – the P2Y family of G-protein-coupled receptors and the P2X family of ligand-gated cation channels. Multiple P2...

...Erina Takai; Mitsutoshi Tsukimoto; Hitoshi Harada; Keisuke Sawada; Yoshinori Moriyama; Shuji Kojima Summary TGF-β1 plays a key role in cancer progression through induction of various biological effects, including cell migration. Extracellular nucleotides, such as ATP, released from cells play...

...D. MacMillan; C. Kennedy; J. G. McCarron Summary Adenosine 5′-triphosphate (ATP) mediates a variety of biological functions following nerve-evoked release, via activation of either G-protein-coupled P2Y- or ligand-gated P2X receptors. In smooth muscle, ATP, acting via P2Y receptors (P2YR), may act...

... measurement of [Ca 2+ ] i and extracellular ATP revealed a concomitant secretion of ATP during GPN-evoked Ca 2+ signalling. Furthermore, the ability of GPN to raise [Ca 2+ ] i was inhibited by P2Y receptor antagonists or by inhibiting vesicular exocytosis with N -ethylmaleimide (NEM). NEM treatment...

.... Neuronal ATP levels in soma and dendrites decreased significantly during prolonged seizures and correlated with the frequency of the oscillatory Ca 2+ signal, indicative of activity-dependent ATP consumption. Blocking mitochondrial complex I, complex V or uncoupling mitochondrial oxidative phosphorylation...

...-deficient neurons have been shown to exhibit a low Δψm. PINK1 deficiency induces impaired respiration in both cell types, with a concomitant increase in glycolytic activity. We demonstrate that the high glycolytic capacity in myocytes compared with neurons enables them to produce more ATP and, therefore...

... required to promote adhesion remains unclear. Purinergic P2X7 receptors are important for astrocyte function and form large non-selective cation pores upon binding to their ligand, ATP. Thus, we evaluated whether the intracellular calcium required for Thy-1-induced cell adhesion stems from influx mediated...

... the activation of an intercellular signaling pathway in sea urchin embryos by membrane wounding that evokes Ca 2+ spikes in neighboring cells. This pathway was mimicked by ATP application, and inhibited by apyrase, cadmium, and ω-agatoxin-IVA. Microinjection of dysferlin antisense phosphorodiamidate morpholino...

.... Here, serum or sphingosine-1-phosphate stimulation of J774 and RAW macrophages released ADP within seconds into the extracellular medium, along with an adenylate kinase activity that converted ADP to ATP. ATP then activated the P2X7 receptor (P2X7R) that was necessary for a peak of plasma-membrane...

...-phosphate and phosphatidic acid – stimulate both actin assembly and transport of ADP across the phagosomal membrane into the lumen. Once there, this ADP is converted to ATP by adenylate kinase activity. High luminal ATP concentrations correlated well with phagosome actin assembly under different conditions...

...Julieta Schachter; Andressa Piedade Motta; Aliane de Souza Zamorano; Hercules Antônio da Silva-Souza; Marilia Zaluar P. Guimarães; Pedro Muanis Persechini Macrophages express the P2X 7 receptor and other nucleotide (P2) receptors, and display the phenomenon of extracellular ATP (ATP e )-induced...

..., the initial signal to trigger these signaling events in response to cell injury remains elusive. In the present study, we investigated the role of ATP released from the injured cells in EGFR transactivation in HCE cells as well as in BEAS 2B cells, a bronchial epithelial cell line. Wounding of epithelial...

... that, once activated by the binding of extracellular ATP, lead to the opening of transmembrane pores permeable to molecules of up to 900 Da. There is evidence suggesting an interplay between gap junctions and P2 receptors in different cell systems. Thus, we used ATP-sensitive and -insensitive J774.G8...